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Hexokinase Type III Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: Hexokinase Type III Antibody [NBP2-38799] - Analysis in human bone marrow and kidney tissues using NBP2-38799 antibody. Corresponding HK3 RNA-seq data are ...read more
Immunohistochemistry-Paraffin: Hexokinase Type III Antibody [NBP2-38799] - Staining of human bone marrow shows high expression.
Western Blot: Hexokinase Type III Antibody [NBP2-38799] -Analysis in human spleen tissue.
Immunohistochemistry-Paraffin: Hexokinase Type III Antibody [NBP2-38799] -Staining of human lymphoid tissues shows strong cytoplasmic positivity in non-germinal center cells.
Immunohistochemistry-Paraffin: Hexokinase Type III Antibody [NBP2-38799] -Staining of human colon shows strong cytoplasmic positivity in lymphoid cells.
Immunohistochemistry-Paraffin: Hexokinase Type III Antibody [NBP2-38799] -Staining of human skin shows negative cytoplasmic positivity in squamous epithelial cells as expected.
Immunohistochemistry-Paraffin: Hexokinase Type III Antibody [NBP2-38799] -Staining of human kidney shows negative cytoplasmic positivity in cells in tubules and cells in glomeruli as expected.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

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Hexokinase Type III Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: MDSIGSSGLRQGEETLSCSEEGLPGPSDSSELVQECLQQFKVTRAQLQQIQASLLGSMEQA
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HK3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
Hexokinase Type III Protein (NBP2-38799PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Hexokinase Type III Antibody

  • ATP:D-hexose 6-phosphotransferase
  • EC 2.7.1
  • EC 2.7.1.1
  • hexokinase 3 (white cell)
  • Hexokinase Type III
  • hexokinase-3
  • Hexokinase-C
  • HK III
  • HK3
  • HKIII
  • HXK3

Background

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 3. Similar to hexokinases 1 and 2, this allosteric enzyme is inhibited by its product glucose-6-phosphate. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol HK3
Uniprot