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![Western Blot: Hexokinase 2 Antibody (OTI4C5) [NBP2-02272] - Glycolysis-related genes and proteins were upregulated in differentiated podocytes. Representative blot images of glycolysis-related proteins (n = 3) (GLUT4 - NBP1-49533; HK2/Hexokinase 2 - NBP2-02272). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41419-020-2481-5), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-OTI4C5-Western-Blot-NBP2-02272-img0021.jpg "Western Blot: Hexokinase 2 Antibody (OTI4C5) [NBP2-02272] - Glycolysis-related genes and proteins were upregulated in differentiated podocytes. Representative blot images of glycolysis-related proteins (n = 3) (GLUT4 - NBP1-49533; HK2/Hexokinase 2 - NBP2-02272). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41419-020-2481-5), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Hexokinase 2 Antibody (OTI4C5) [NBP2-02272] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Hexokinase 2.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-OTI4C5-Immunocytochemistry-Immunofluorescence-NBP2-02272-img0012.jpg)
![Flow Cytometry: Hexokinase 2 Antibody (OTI4C5) [NBP2-02272] - HEK293T cells transfected with either pCMV6-ENTRY Hexokinase 2.(Red) or empty vector control plasmid (Blue) were immunostained with anti-Hexokinase 2 mouse monoclonal, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-OTI4C5-Flow-Cytometry-NBP2-02272-img0019.jpg)
![Western Blot: Hexokinase 2 Antibody (OTI4C5) [NBP2-02272] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Hexokinase 2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Hexokinase 2.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-OTI4C5-Western-Blot-NBP2-02272-img0017.jpg)
![Western Blot: Hexokinase 2 Antibody (OTI4C5) [NBP2-02272] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Hexokinase 2I monoclonal antibody.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-OTI4C5-Western-Blot-NBP2-02272-img0018.jpg)
![Immunocytochemistry/Immunofluorescence: Hexokinase 2 Antibody (OTI4C5) [NBP2-02272] - Staining of HepG2 cells using anti-Hexokinase 2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-OTI4C5-Immunocytochemistry-Immunofluorescence-NBP2-02272-img0020.jpg)
![Western Blot: Hexokinase 2 Antibody (3D3) [NBP1-51643] - Analysis using HK2 mouse mAb against Jurkat (1), Hela (2) and HEK293 (3) cell lysate.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-3D3-Western-Blot-NBP1-51643-img0010.jpg "Western Blot: Hexokinase 2 Antibody (3D3) [NBP1-51643] - Analysis using HK2 mouse mAb against Jurkat (1), Hela (2) and HEK293 (3) cell lysate.")
![Immunohistochemistry: Hexokinase 2 Antibody (3D3) [NBP1-51643] - Images of IHC analysis of B7-H3 and HK2 protein expression and hematoxylin and eosin (H&E) staining of CRC (n=126) tissue sections. One representative image is shown. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41419-019-1549-6), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-3D3-Immunohistochemistry-NBP1-51643-img0012.jpg)
![Flow Cytometry: Hexokinase 2 Antibody (3D3) [NBP1-51643] - Analysis of K562 cells using HK2 mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-3D3-Flow-Cytometry-NBP1-51643-img0007.jpg)
![Immunohistochemistry-Paraffin: Hexokinase 2 Antibody (3D3) [NBP1-51643] - Analysis of esophagus cancer tissues (left) and human lung cancer (right) using HK2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-3D3-Immunohistochemistry-Paraffin-NBP1-51643-img0009.jpg)
![<b>Genetic Strategies Validation. </b>Knockdown Validated: Hexokinase 2 Antibody (3D3) [NBP1-51643] - The protein level of HK2 in B7-H3-overexpressing HCT116 or RKO cells after transfection with siRNA negative control (NC) or HK2 siRNA transfection were analyzed by western blot. beta-actin served as a loading control. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41419-019-1549-6), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-3D3-Knockdown-Validated-NBP1-51643-img0011.jpg)
![Western Blot: Hexokinase 2 Antibody (3D3) - BSA Free [NBP1-51643] - B7-H3 regulated glycolysis through HK2.a The protein level of HK2 in B7-H3-overexpressing HCT116 or RKO cells after transfection with siRNA negative control (NC) or HK2 siRNA transfection were analyzed by western blot. beta -actin served as a loading control. b, c Glucose consumption (b) & lactate production (c) were measured in B7-H3-overexpressing HCT116 or RKO cells transfected with NC or HK2 siRNA. d, e Glucose consumption (d) & lactate production (e) were measured in B7-H3-overexpressing HCT116 or RKO cells treated with PBS or 2-DG. Values are expressed as means (SEMs). Five samples were analyzed per condition, & the experiments were performed in triplicate. *P < 0.05 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30952834), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-51643_mouse-monoclonal-hexokinase-2-antibody-3d3-310202415175275.jpg)
![Western Blot: Hexokinase 2 Antibody (3D3) - BSA Free [NBP1-51643] - B7-H3 promoted the expression of HK2 in CRC cells.a, b The expression of glycolysis-related genes was detected by RT-qPCR in both B7-H3-overexpressing HCT116 (a) & RKO (b) cells. c, d The mRNA level of HK2 was detected by RT-qPCR in both HCT116 (c) & RKO (d) cells after transfection with siRNA NC, B7-H3 siRNA-1 or B7-H3 siRNA-2. e HK2 protein level & STAT3 activation (examined by the p-STAT3 expression level) were detected by western blot in both HCT116 & RKO cells after transfection with NC, B7-H3 siRNA-1 or B7-H3 siRNA-2. beta -actin served as a loading control. f HK2 protein level & STAT3 activation (examined by the p-STAT3 expression level) were detected by western blot in both B7-H3-overexpressing HCT116 & RKO cells. beta -actin served as a loading control. g Schematic representation of the proposed B7-H3/STAT3/HK2 axis. Values are expressed as means (SEMs). Five samples were analyzed per condition, & the experiments were performed in triplicate. *P < 0.05 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30952834), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-51643_mouse-monoclonal-hexokinase-2-antibody-3d3-310202416173538.jpg)
![Western Blot: Hexokinase 2 Antibody [NBP2-16814] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Hk2 antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-Western-Blot-NBP2-16814-img0021.jpg "Western Blot: Hexokinase 2 Antibody [NBP2-16814] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Hk2 antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: Hexokinase 2 Antibody [NBP2-16814] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Hexokinase II stained by Hexokinase II antibody diluted at 1:2000. Blue: Fluoroshield with DAPI. Scale bar= 10 um.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16814-img0017.jpg)
![Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814] - Mouse muscle. Hexokinase II stained by Hexokinase II antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-Immunohistochemistry-Paraffin-NBP2-16814-img0022.jpg)
![Western Blot: Hexokinase 2 Antibody [NBP2-16814] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Hexokinase II antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-Western-Blot-NBP2-16814-img0019.jpg)
![Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814] - U87 xenograft. Hexokinase II antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Hexokinase-II-Antibody-Immunohistochemistry-Paraffin-NBP2-16814-img0003.jpg)
![Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814] - Mouse muscle. Hexokinase II antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-Immunohistochemistry-Paraffin-NBP2-16814-img0005.jpg)
![Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814] - Rat heart. Hexokinase II antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-Immunohistochemistry-Paraffin-NBP2-16814-img0007.jpg)
![Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814] - Rat stomach. Hexokinase II antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-Immunohistochemistry-Paraffin-NBP2-16814-img0008.jpg)
![Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814] - Rat colon. Hexokinase II stained by Hexokinase II antibody diluted at 1:2000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-Immunohistochemistry-Paraffin-NBP2-16814-img0012.jpg)
![Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814] - Rat kidney. Hexokinase II stained by Hexokinase II antibody diluted at 1:2000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-Immunohistochemistry-Paraffin-NBP2-16814-img0013.jpg)
![Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814] - Mouse heart. Hexokinase II stained by Hexokinase II antibody diluted at 1:2000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-Immunohistochemistry-Paraffin-NBP2-16814-img0014.jpg)
![Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814] - Rat muscle. Hexokinase II stained by Hexokinase II antibody diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-Immunohistochemistry-Paraffin-NBP2-16814-img0015.jpg)
![Western Blot: Hexokinase 2 Antibody (4H1) [H00003099-M01] - HK2 monoclonal antibody (M01), clone 4H1. Analysis of HK2 expression in A-431.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-4H1-Western-Blot-H00003099-M01-img0007.jpg "Western Blot: Hexokinase 2 Antibody (4H1) [H00003099-M01] - HK2 monoclonal antibody (M01), clone 4H1. Analysis of HK2 expression in A-431.")
![Western Blot: Hexokinase 2 Antibody (4H1) [H00003099-M01] - HK2 monoclonal antibody (M01), clone 4H1. Analysis of HK2 expression in PC-12.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-4H1-Western-Blot-H00003099-M01-img0006.jpg)
![Sandwich ELISA: Hexokinase 2 Antibody (4H1) [H00003099-M01] - Detection limit for recombinant GST tagged HK2 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-4H1-Sandwich-ELISA-H00003099-M01-img0005.jpg)
![Western Blot: Hexokinase 2 Antibody [NBP2-44234] - Western Blot of Rabbit Hexokinase 2 2 antibody. Lane 1: Molecular Weight Marker. Lane 2: Hexokinase 2. Primary Antibody: Anti-Hexokinase 2 at 1:1000 overnight in 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP at 1:40,000 for 30 mins at RT. Block: Blocking buffer for 30 mins at RT. Expect MW: ~54kDa.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-Western-Blot-NBP2-44234-img0002.jpg "Western Blot: Hexokinase 2 Antibody [NBP2-44234] - Western Blot of Rabbit Hexokinase 2 2 antibody. Lane 1: Molecular Weight Marker. Lane 2: Hexokinase 2. Primary Antibody: Anti-Hexokinase 2 at 1:1000 overnight in 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP at 1:40,000 for 30 mins at RT. Block: Blocking buffer for 30 mins at RT. Expect MW: ~54kDa.")
![ELISA: Hexokinase 2 Antibody [NBP2-44234] - ELISA Results of Rabbit Hexokinase 2 2 antibody tested against purified Hexokinase 2. Each well was coated in duplicate with 1.0 ug of Hexokinase 2 (Yeast). The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer, Goat Anti-Rabbit IgG HRP conjugated and TMB substrate.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-ELISA-NBP2-44234-img0001.jpg)
![Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 | Lane 2: RT4 | Lane 3: U-251 MG | Lane 4: Human Plasma | Lane 5: Liver | Lane 6: Tonsil](http://images.novusbio.com/fullsize/nbp3-21294_rabbit-hexokinase-2-pab-4520231552913.jpg)
![Western Blot: Hexokinase 2 Antibody (5Z8C3) [NBP3-33349] - Western blot analysis of various lysates using [KO Validated] Hexokinase 2 Rabbit mAb at 1:25000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-33349_rabbit-hexokinase-2-mab-5z8c3-101220241214436.jpg "Western Blot: Hexokinase 2 Antibody (5Z8C3) [NBP3-33349] - Western blot analysis of various lysates using [KO Validated] Hexokinase 2 Rabbit mAb at 1:25000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.")
![Western Blot: Hexokinase 2 Antibody (5Z8C3) [NBP3-33349] - Western blot analysis of lysates from Mouse skeletal muscle using [KO Validated] Hexokinase 2 Rabbit mAb at 1:25000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-33349_rabbit-hexokinase-2-mab-5z8c3-1012202411531054.jpg)
![Western Blot: Hexokinase 2 Antibody (1A7) [NBP1-04296] - A): Recombinant protein (20ng or 100ng) of Hexokinase four isoform were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Hexokinase I, II (1:1000). (B): Cell lysates of Jurkat (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Hexokinase I, II (1:2,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-1A7-Western-Blot-NBP1-04296-img0003.jpg "Western Blot: Hexokinase 2 Antibody (1A7) [NBP1-04296] - A): Recombinant protein (20ng or 100ng) of Hexokinase four isoform were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Hexokinase I, II (1:1000). (B): Cell lysates of Jurkat (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Hexokinase I, II (1:2,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection.")
![Western Blot: Hexokinase 2 Antibody [NBP1-90922] - Analysis in human cell line U-87 MG.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-Western-Blot-NBP1-90922-img0005.jpg "Western Blot: Hexokinase 2 Antibody [NBP1-90922] - Analysis in human cell line U-87 MG.")
![Western Blot: Hexokinase II Overexpression Lysate (Adult Normal) [NBL1-11579] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Hexokinase II.](http://images.novusbio.com/fullsize/Hexokinase-II-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-11579-img0002.jpg "Western Blot: Hexokinase II Overexpression Lysate (Adult Normal) [NBL1-11579] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Hexokinase II.")
![ELISA: Human Hexokinase 2 ELISA Kit (Colorimetric) [NBP2-60572]](http://images.novusbio.com/fullsize/Human-Hexokinase-2-ELISA-Kit-Colorimetric-ELISA-NBP2-60572-img0001.jpg "ELISA: Human Hexokinase 2 ELISA Kit (Colorimetric) [NBP2-60572]")