![Western Blot: Hexokinase 2 Antibody [NBP2-16814] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Hk2 antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-Western-Blot-NBP2-16814-img0021.jpg "Western Blot: Hexokinase 2 Antibody [NBP2-16814] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Hk2 antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
| Reactivity | Hu, Mu, Rt, PoSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Hexokinase 2. The exact sequence is proprietary. |
| Localization | Mitochondrion outer membrane (By similarity) |
| Predicted Species | Porcine (96%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | HK2 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 102 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS, 20% Glycerol |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
| Publication using NBP2-16814 | Applications | Species |
|---|---|---|
| Torres A, Kang S, Mahony CB et al. Role of mitochondria-bound HK2 in rheumatoid arthritis fibroblast-like synoviocytes Frontiers in Immunology 2023-07-17 [PMID: 37529037] |
Secondary Antibodies |
Isotype Controls |
Research Areas for Hexokinase 2 Antibody (NBP2-16814)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: Hexokinase 2 Antibody [NBP2-16814] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Hexokinase II stained by Hexokinase II antibody diluted at 1:2000. Blue: Fluoroshield with DAPI. Scale bar= 10 um.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16814-img0017.jpg "Immunocytochemistry/Immunofluorescence: Hexokinase 2 Antibody [NBP2-16814] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Hexokinase II stained by Hexokinase II antibody diluted at 1:2000. Blue: Fluoroshield with DAPI. Scale bar= 10 um.")
![Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814] - Mouse muscle. Hexokinase II stained by Hexokinase II antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-Immunohistochemistry-Paraffin-NBP2-16814-img0022.jpg "Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814] - Mouse muscle. Hexokinase II stained by Hexokinase II antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Western Blot: Hexokinase 2 Antibody [NBP2-16814] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Hexokinase II antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-Western-Blot-NBP2-16814-img0019.jpg "Western Blot: Hexokinase 2 Antibody [NBP2-16814] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Hexokinase II antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814] - U87 xenograft. Hexokinase II antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Hexokinase-II-Antibody-Immunohistochemistry-Paraffin-NBP2-16814-img0003.jpg "Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814] - U87 xenograft. Hexokinase II antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814] - Mouse muscle. Hexokinase II antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-Immunohistochemistry-Paraffin-NBP2-16814-img0005.jpg "Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814] - Mouse muscle. Hexokinase II antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814] - Rat heart. Hexokinase II antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-Immunohistochemistry-Paraffin-NBP2-16814-img0007.jpg "Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814] - Rat heart. Hexokinase II antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814] - Rat stomach. Hexokinase II antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-Immunohistochemistry-Paraffin-NBP2-16814-img0008.jpg "Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814] - Rat stomach. Hexokinase II antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814] - Rat colon. Hexokinase II stained by Hexokinase II antibody diluted at 1:2000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-Immunohistochemistry-Paraffin-NBP2-16814-img0012.jpg "Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814] - Rat colon. Hexokinase II stained by Hexokinase II antibody diluted at 1:2000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814] - Rat kidney. Hexokinase II stained by Hexokinase II antibody diluted at 1:2000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-Immunohistochemistry-Paraffin-NBP2-16814-img0013.jpg "Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814] - Rat kidney. Hexokinase II stained by Hexokinase II antibody diluted at 1:2000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814] - Mouse heart. Hexokinase II stained by Hexokinase II antibody diluted at 1:2000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-Immunohistochemistry-Paraffin-NBP2-16814-img0014.jpg "Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814] - Mouse heart. Hexokinase II stained by Hexokinase II antibody diluted at 1:2000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814] - Rat muscle. Hexokinase II stained by Hexokinase II antibody diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Hexokinase-2-Antibody-Immunohistochemistry-Paraffin-NBP2-16814-img0015.jpg "Immunohistochemistry-Paraffin: Hexokinase 2 Antibody [NBP2-16814] - Rat muscle. Hexokinase II stained by Hexokinase II antibody diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
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![Western Blot Androgen R/NR3C4 [p Ser213, p Ser210] Antibody (156C135.2) - BSA Free](https://images.novusbio.com/images/Androgen-R-NR3C4-p-Ser213-p-Ser210-Antibody-156C135-2-Western-Blot-NB100-56603-img0004.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")