| Ma T, Jin Y, Wang S et Al. Innovation in CRC Research: Targeting SPACA6P-AS for Progression J Biochem Mol Toxicol 2024-12-10 [PMID: 39655708] |
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| Duque J, Bonfanti A, Fouchard J et Al. Rupture strength of living cell monolayers Nat Mater 2024-10-31 [PMID: 39468334] |
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| Nagata M, Carvalho Schäfer Y, Wachsmuth L, Pasparakis M. et Al. A shorter splicing isoform antagonizes ZBP1 to modulate cell death and inflammatory responses EMBO J 2024-09-19 [PMID: 39300211] |
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| Higashi, Y;Dashek, R;Delafontaine, P;Rector, RS;Chandrasekar, B; EF24, a Curcumin Analog, Reverses Interleukin-18-Induced miR-30a or miR-342-Dependent TRAF3IP2 Expression, RECK Suppression, and the Proinflammatory Phenotype of Human Aortic Smooth Muscle Cells Cells 2024-10-10 [PMID: 39451191] |
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| Shin SH, Lee YE, Yoon HN et al. An innovative strategy harnessing self-activating CAR-NK cells to mitigate TGF-beta 1-driven immune suppression. Biomaterials 2024-10-10 [PMID: 39423512] |
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| Ianevski A, Nader K, Driva K et al. Single-cell transcriptomes identify patient-tailored therapies for selective co-inhibition of cancer clones Nature Communications 2024-10-03 [PMID: 39362905] |
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| Crapser Jd Investigating microglial regulation of the extracellular matrix in health and neurodegenerative disease Award 2021-01-01 (WB) |
WB |
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| Augi�re C, Campolina-Silva G, Vijayakumaran A et al. ARL13B controls male reproductive tract physiology through primary and Motile Cilia Communications Biology 2024-10-14 [PMID: 39397107] |
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| Lehle, JD;Lin, YH;Gomez, A;Chavez, L;McCarrey, JR; An in vitro approach reveals molecular mechanisms underlying endocrine disruptor-induced epimutagenesis eLife 2024-10-03 [PMID: 39361026] |
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| Hung HC, Tan BF, Lin WS et Al. Glycan masking of NTD loops with a chimeric RBD of the spike protein as a vaccine design strategy against emerging SARS-CoV-2 Omicron variants J Med Virol 2024-08-28 [PMID: 39192804] |
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| Wang H, Martinez Yus M, Brady T et Al. Sex differences and role of lysyl oxidase-like 2 in angiotensin II-induced hypertension in mice Am J Physiol Heart Circ Physiol 2024-09-03 [PMID: 39028284] |
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| Steppan J, Wang H, Nandakumar K et Al. LOXL2 inhibition ameliorates pulmonary artery remodeling in pulmonary hypertension Am J Physiol Lung Cell Mol Physiol 2024-09-20 [PMID: 39010824] |
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| Kim E, Kim HK, Sul JH et al. Extracellular Vesicles Derived from Adipose Stem Cells Alleviate Systemic Sclerosis by Inhibiting TGF-beta Pathway. Biomolecules & therapeutics 2024-07-01 [PMID: 38835111] |
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| Poongodi R, Yang TH, Huang YH et Al. Stem cell exosome-loaded Gelfoam improves locomotor dysfunction and neuropathic pain in a rat model of spinal cord injury Stem Cell Res Ther 2024-05-20 [PMID: 38764049] |
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| Duan M, Ru X, Zhou J et Al. Endothelial EGLN3-PKM2 signaling induces the formation of acute astrocytic barrier to alleviate immune cell infiltration after subarachnoid hemorrhage Fluids Barriers CNS 2024-05-16 [PMID: 38755642] |
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| Imai T, Lin J, Kaya GG et al. The RIPK1 death domain restrains ZBP1- and TRIF-mediated cell death and inflammation. Immunity 2024-07-10 [PMID: 38744293] |
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| Zhong L. microRNAs mediating E-selectin-dependent metastatic abilities of colon cancer cells and the signaling mechanisms behind their regulation. Thesis 1905-07-10 (WB, Human) |
WB |
Human |
| Laura Pelz, Laura Dossou, Nine Kompier, René Jüttner, Gabrielle Siemonsmeier, Niklas Meyer, Elijah D. Lowenstein, Ines Lahmann, Helmut Kettenmann, Carmen Birchmeier, Fritz G. Rathjen The IgCAM BT-IgSF (IgSF11) Is Essential for Connexin43-Mediated Astrocyte–Astrocyte Coupling in Mice eNeuro 2024-02-22 [PMID: 38388443] |
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| Liu YN, Chen WY, Liu MK et Al. Immunosuppressive role of BDNF in therapy-induced neuroendocrine prostate cancer Mol Oncol 2024-02-21 [PMID: 38381121] |
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| Joshua C. Chang, Molly R. Ryan, Marie C. Stark, Su Liu, Pravinkumar Purushothaman, Fria Bolan, Caitlin A. Johnson, Mark Champe, Hui Meng, Michael W. Lawlor, Sarah Halawani, Lucie V. Ngaba, David R. Lynch, Crystal Davis, Elena Gonzalo-Gil, Cathleen Lutz, Fabrizia Urbinati, Bala Medicherla, Carlos Fonck AAV8 gene therapy reverses cardiac pathology and prevents early mortality in a mouse model of Friedreich’s ataxia Molecular Therapy. Methods & Clinical Development 2024-01-22 [PMID: 38352270] |
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| Chen, HH;Nguyen, TV;Shih, YH;Chang, KC;Chiu, KC;Hsia, SM;Fuh, LJ;Shieh, TM; Combining microfluidic chip and low-attachment culture devices to isolate oral cancer stem cells Journal of dental sciences 2024-01-01 [PMID: 38303836] |
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| Laura Jahnke, Virginie Perrenoud, Souska Zandi, Yuebing Li, Federica Maria Conedera, Volker Enzmann, Girish Kumar Srivastava Modulation of Extracellular Matrix Composition and Chronic Inflammation with Pirfenidone Promotes Scar Reduction in Retinal Wound Repair Cells 2024-01-16 [PMID: 38247855] |
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| Kirkbride JA, Nilsson GY, Kim JI et Al. PHI-1, an Endogenous Inhibitor Protein for Protein Phosphatase-1 and a Pan-Cancer Marker, Regulates Raf-1 Proteostasis Biomolecules 2023-12-25 [PMID: 38136612] |
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Steppan J, Wang H, Nandakumar K et al. LOXL2 inhibition ameliorates pulmonary artery remodeling in pulmonary hypertension bioRxiv 2023-10-29 [PMID: 37961202] (WB)
Details:
Dilution 1:5000 |
WB |
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| Kong MJ, Han SJ, Seu SY et Al. High water intake induces primary cilium elongation in renal tubular cells Kidney Res Clin Pract 2024-05-01 [PMID: 37933114] |
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| Chua B, Ferry L, Domrane C et al. E4F1 and ZNF148 are transcriptional activators of the A57C and wildtypeTERTpromoter bioRxiv 2023-01-15 [PMID: 37918959] (WB, Human) |
WB |
Human |
| Arumugam TV, Alli-Shaik A, Liehn EA et al. Multiomics analyses reveal dynamic bioenergetic pathways and functional remodeling of the heart during intermittent fasting Elife 2023-09-28 [PMID: 37769126] |
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Kalinin S, Feinstein DL Astrocyte lipocalin-2 modestly effects disease severity in a mouse model of multiple sclerosis while reducing mature oligodendrocyte protein and mRNA expression Neuroscience letters 2023-09-23 [PMID: 37748675] (IHC-P, Mouse)
Details:
1:300 IHC-P dilution |
IHC-P |
Mouse |
| Guo D, Yu M, Guo H et al. Panax notoginseng saponins inhibits oxidative stress- induced human nucleus pulposus cell apoptosis and delays disc degeneration in vivo and in vitro Journal of ethnopharmacology 2023-09-15 [PMID: 37716491] (WB, Human) |
WB |
Human |
| Yasuhara J, Manivannan SN, Majumdar U et al. Novel pathogenic GATA6 variant associated with congenital heart disease, diabetes mellitus and necrotizing enterocolitis Pediatric research 2023-09-12 [PMID: 37700164] |
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| Huang CL, Chen DY, Tzang CC et al. Celastrol attenuates human parvovirus B19 NS1?induced NLRP3 inflammasome activation in macrophages Molecular Medicine Reports 2023-08-31 [PMID: 37654202] (Western Blot) |
Western Blot |
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| Cheng W, Lawson C, Liu H et al. Exploration of Mediators Associated with Myocardial Remodelling in Feline Hypertrophic Cardiomyopathy Animals 2023-06-26 [PMID: 37443910] (WB, Feline) |
WB |
Feline |
| Kong MJ, Han SJ, Seu SY et al. Shortening of primary cilia length is associated with urine concentration in the kidneys Kidney Research and Clinical Practice 2023-05-31 [PMID: 37313611] (Western Blot) |
Western Blot |
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| Natsagdorj A, Izumi K, Hiratsuka K et al. The CCL2-CCR2 Axis Contributes to Migration of Cabazitaxel-resistant Prostate Cancer Cells Anticancer research 2023-06-01 [PMID: 37247914] |
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| Abe T, Sato T, Murotomi K Sudachitin and Nobiletin Stimulate Lipolysis via Activation of the cAMP/PKA/HSL Pathway in 3T3-L1 Adipocytes Foods (Basel, Switzerland) 2023-05-10 [PMID: 37238764] (Western Blot, Mouse) |
Western Blot |
Mouse |
| Wen YC, Tram VTN, Chen WH et al. CHRM4/AKT/MYCN upregulates interferon alpha-17 in the tumor microenvironment to promote neuroendocrine differentiation of prostate cancer Cell death & disease 2023-05-04 [PMID: 37142586] (ChIP, Human) |
ChIP |
Human |
| Azman MS, Alard EL, Dodel M et al. An ERK1/2-driven RNA-binding switch in nucleolin drives ribosome biogenesis and pancreatic tumorigenesis downstream of RAS oncogene The EMBO journal 2023-04-11 [PMID: 37039106] (WB, Mouse) |
WB |
Mouse |
| Bachmann S, Bell M, Klimek J, Zempel H Subcellular localization of TAU isoforms and their influence on microtubule dynamics bioRxiv 2020-06-17 |
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| Jankauskas SS, Mone P, Avvisato R et al. miR-181c targets Parkin and SMAD7 in human cardiac fibroblasts: Validation of differential microRNA expression in patients with diabetes and heart failure with preserved ejection fraction Mechanisms of ageing and development 2023-04-26 [PMID: 37116731] |
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| Park YR, Kong MJ, Noh MR, Park KM Rac1 inhibition protects the kidney against kidney ischemia/reperfusion through the inhibition of macrophage migration The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology 2023-05-01 [PMID: 37078299] (WB, Mouse) |
WB |
Mouse |
| Wang H, Poe A, Martinez Yus M et al. Lysyl oxidase-like 2 processing by factor Xa modulates its activity and substrate preference Communications biology 2023-04-07 [PMID: 37029269] (WB) |
WB |
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| Kadhim H Functional and Physiological Role of Extra-Hypothalamic Corticotropin Releasing Hormone Neurons in the Nucleus of the Hippocampal Commissure in Regulation of Stress Response Thesis Jan 1 2020 (WB) |
WB |
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| Aggarwal M. 2,2-Diphenethyl Isothiocyanate Enhances Topoisomerase Inhibitor-Induced Cell Death and Suppresses Multi-Drug Resistance 1 in Breast Cancer Cells Cancers (Basel) 2023-02-01 [PMID: 36765888] |
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| Yamamoto Y, Kurokawa M, Ogawa T et al. Involvement of gap junctions in the increased contraction of bovine oviducts at pre-ovulation observed in vitro Reproduction (Cambridge, England) 2023-03-01 [PMID: 36598933] |
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| Kalkan H, Pagano E, Paris D et al. Targeting gut dysbiosis against inflammation and impaired autophagy in Duchenne muscular dystrophy EMBO Molecular Medicine 2023-03-08 [PMID: 36594243] (Western Blot, Block/Neutralize) |
Western Blot, Block/Neutralize |
|
| Masala N, Pofahl M, Haubrich AN et al. Targeting aberrant dendritic integration to treat cognitive comorbidities of epilepsy Brain 2023-06-01 [PMID: 36448426] (Western Blot, Block/Neutralize) |
Western Blot, Block/Neutralize |
|
| Arias-Garcia M, Rickman R, Sero J The cell-cell adhesion protein JAM3 determines nuclear deformability by regulating microtubule organization biorxiv.org Jan 1 2019 (WB, Human) |
WB |
Human |
| Bonham C, Mandati V, Singh R et al. Coupling substrate-trapping with proximity-labeling to identify protein tyrosine phosphatase PTP1B signaling networks Journal of Biological Chemistry 2023-03-01 [PMID: 36871762] |
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| Dufour D, Dumontet T, Sahut-Barnola I et al. Loss of SUMO-specific protease 2 causes isolated glucocorticoid deficiency by blocking adrenal cortex zonal transdifferentiation Research Square 2022-03-18 [PMID: 36543805] (WB, Mouse) |
WB |
Mouse |
| Majumdar U, Choudhury TZ, Manivannan S et al. Single-cell RNA-sequencing analysis of aortic valve interstitial cells demonstrates the regulation of integrin signaling by nitric oxide Frontiers in Cardiovascular Medicine 2022-10-25 [PMID: 36386365] (Western Blot) |
Western Blot |
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| |
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| Ivan Mestres, Federico Calegari 4931414P19Rik, a microglia chemoattractant secreted by neural progenitors, modulates neuronal migration during corticogenesis Development (Cambridge, England) 2023-05-01 [PMID: 37070770] |
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| Yeung CC, Olesen AT, Wilson R et al. Proteome profiles of intramuscular connective tissue: influence of aging and physical training Journal of applied physiology (Bethesda, Md. : 1985) 2023-05-01 [PMID: 36995911] |
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| Jankauskas SS, Kansakar U, Sardu C et al. COVID-19 Causes Ferroptosis and Oxidative Stress in Human Endothelial Cells Antioxidants (Basel, Switzerland) 2023-01-31 [PMID: 36829885] (WB, Human) |
WB |
Human |
| Kim EJ, Yoon JU, Kim CH et al. Lidocaine inhibits osteogenic differentiation of human dental pulp stem cells in vitro The Journal of international medical research 2023-02-01 [PMID: 36748349] (WB, Human) |
WB |
Human |
| Abe T Timing of Medium-Chain Triglyceride Consumption Modulates Effects in Mice with Obesity Induced by a High-Fat High-Sucrose Diet Nutrients 2022-12-01 [PMID: 36501131] (WB, Mouse) |
WB |
Mouse |
| Abe T Timing of Medium-Chain Triglyceride Consumption Modulates Effects in Mice with Obesity Induced by a High-Fat High-Sucrose Diet Nutrients 2022-12-01 [PMID: 36501131] (WB, Mouse) |
WB |
Mouse |
| Reuter SP, Soonpaa MH, Field D et al. Cardiac Troponin I-interacting Kinase Impacts Cardiomyocyte S-phase Activity But Not Cardiomyocyte Proliferation Circulation 2022-11-16 [PMID: 36382596] |
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| Wang YC, Tsai MC, Chen YS et al. NPRL2 down-regulation facilitates the growth of hepatocellular carcinoma via the mTOR pathway and autophagy suppression Hepatology communications 2022-12-01 [PMID: 36321403] (WB, Human) |
WB |
Human |
| Griem-Krey N, Gauger SJ, Gowing EK et al. The CaMKIIa hub ligand Ph-HTBA promotes neuroprotection after focal ischemic stroke by a distinct molecular interaction Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2022-10-20 [PMID: 36274464] (WB, Mouse) |
WB |
Mouse |
| Gerwinn T, Salemi S, Schori LJ et al. Improved contractile potential in detrusor microtissues from pediatric patients with end stage lower urinary tract dysfunction Frontiers in Cell and Developmental Biology 2022-10-04 [PMID: 36268506] (Simple Western, Immunocytochemistry/ Immunofluorescence) |
Simple Western, Immunocytochemistry/ Immunofluorescence |
|
| Kansakar U, Gambardella J, Varzideh F et al. miR-142 Targets TIM-1 in Human Endothelial Cells: Potential Implications for Stroke, COVID-19, Zika, Ebola, Dengue, and Other Viral Infections International Journal of Molecular Sciences 2022-09-06 [PMID: 36142146] (Western Blot) |
Western Blot |
|
| Carew NT, Schmidt HM, Yuan S et al. Loss of cardiomyocyte CYB5R3 impairs redox equilibrium and causes sudden cardiac death Journal of Clinical Investigation 2022-09-15 [PMID: 36106636] |
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Bousgouni V, Inge O, Robertson D et al. ARHGEF9 regulates melanoma morphogenesis in environments with diverse geometry and elasticity by promoting filopodial-driven adhesion iScience 2022-08-19 [PMID: 36039362] (WB, Human)
Details:
Dilution used 1:2000 |
WB |
Human |
| Ross BD, Jang Y, Welton A et al. A lymphatic-absorbed multi-targeted kinase inhibitor for myelofibrosis therapy Nature communications 2022-08-17 [PMID: 35977945] (WB, Mouse) |
WB |
Mouse |
Wavelet-Vermuse C, Odnokoz O, Xue Y et al. CDC20-Mediated hnRNPU Ubiquitination Regulates Chromatin Condensation and Anti-Cancer Drug Response Cancers 2022-07-31 [PMID: 35954396] (WB, Human)
Details:
Dilution used 1:1000 |
WB |
Human |
| Kubota S, Yamamoto Y, Kimura K A Chinese Medicine, Tokishakuyakusan, Increases Bovine Oviductal Tonus via G Protein-Coupled Estrogen Receptor 1 Biological & pharmaceutical bulletin 2022-07-31 [PMID: 35908895] (WB, Bovine) |
WB |
Bovine |
Ojeda-Juarez D, Lawrence J, Soldau K et al. Prions induce an early Arc response and a subsequent reduction in mGluR5 in the hippocampus Neurobiology of disease 2022-07-26 [PMID: 35905927] (WB, Mouse)
Details:
Dilutions: 1:1000 |
WB |
Mouse |
| T Imai, TM Van, M Pasparakis, A Polykratis Smooth muscle cell specific NEMO deficiency inhibits atherosclerosis in ApoE-/- mice Scientific Reports, 2022-07-22;12(1):12538. 2022-07-22 [PMID: 35869246] |
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| Jiao H, Wachsmuth L, Wolf S et al. ADAR1 averts fatal type I interferon induction by ZBP1 Nature 2022-07-01 [PMID: 35859176] (WB, Mouse) |
WB |
Mouse |
| Sugino Y, Sato T, Yamamoto Y, Kimura K. Evaluation of bovine uterine gland functions in 2D and 3D culture system Journal of Reproduction and Development 2022-08-01 [PMID: 35644574] (Western Blot, Block/Neutralize) |
Western Blot, Block/Neutralize |
|
| Kondylis V, Schneider F, Schorn F et al. p62 Promotes Survival and Hepatocarcinogenesis in Mice with Liver-Specific NEMO Ablation Cancers 2022-05-15 [PMID: 35626041] (WB, Mouse) |
WB |
Mouse |
| Mercado-Ay�n E, Warren N, Halawani S et al. Cerebellar Pathology in an Inducible Mouse Model of Friedreich Ataxia Frontiers in Neuroscience 2022-03-24 [PMID: 35401081] (Immunohistochemistry, Western Blot) |
Immunohistochemistry, Western Blot |
|
| Huda N, Khambu B, Liu G et al. Senescence Connects Autophagy Deficiency to Inflammation and Tumor Progression in the Liver Cellular and molecular gastroenterology and hepatology 2022-04-08 [PMID: 35398596] (WB, Mouse) |
WB |
Mouse |
| Stephenson SEM, Costain G, Blok LER et al. Germline variants in tumor suppressor FBXW7 lead to impaired ubiquitination and a neurodevelopmental syndrome American journal of human genetics 2022-04-07 [PMID: 35395208] |
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| Wen YC, Chen WY, Tram VTN et al. Pyruvate kinase L/R links metabolism dysfunction to neuroendocrine differentiation of prostate cancer by ZBTB10 deficiency Cell death & disease 2022-03-19 [PMID: 35306527] (Chemotaxis, Human) |
Chemotaxis |
Human |
| Fan CS, Chen CC, Chen LL et al. Extracellular HSP90 alpha Induces MyD88-IRAK Complex-Associated IKK alpha/beta NF-kappa B/IRF3 and JAK2/TYK2-STAT-3 Signaling in Macrophages for Tumor-Promoting M2-Polarization Cells 2022-01-11 [PMID: 35053345] (WB, Human) |
WB |
Human |
| Faucher-Giguere L, Roy A, Deschamps-Francoeur G et al. High-grade ovarian cancer associated H/ACA snoRNAs promote cancer cell proliferation and survival NAR cancer 2022-03-01 [PMID: 35047824] (WB, Human) |
WB |
Human |
| Bennett JS, Gordon DM, Majumdar U et al. Use of machine learning to classify high-risk variants of uncertain significance in lamin A/C cardiac disease Heart rhythm 2021-12-24 [PMID: 34958940] |
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| Weber RE, Schulze KM, Colburn TD et al. Capillary hemodynamics and contracting skeletal muscle oxygen pressures in male rats with heart failure: Impact of soluble guanylyl cyclase activator Nitric oxide : biology and chemistry 2021-12-04 [PMID: 34871799] (WB, Rat) |
WB |
Rat |
| Xiang Ma, Yusuke Takahashi, Wenjing Wu, Jianglei Chen, Marcus Dehdarani, Wentao Liang, Young-Hwa Shin, Siribhinya Benyajati, Jian-xing Ma Soluble very low-density lipoprotein receptor (sVLDLR) inhibits fibrosis in neovascular age-related macular degeneration FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2022-05-21 [PMID: 34820908] |
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|
| Gerwinn T, Salemi S, Krattiger L Et al. Spheroids of Bladder Smooth Muscle Cells for Bladder Tissue Engineering BioMed research international 2021-11-11 [PMID: 34805410] (Simple Western, Rat) |
Simple Western |
Rat |
| Truong Quang BA, Peters R, Cassani DAD Et al. Extent of myosin penetration within the actin cortex regulates cell surface mechanics Nature communications 2021-11-11 [PMID: 34764258] (WB, Human) |
WB |
Human |
| Heath J, Cheyou ES, Findlay S Et al. POGZ promotes homology-directed DNA repair in an HP1-dependent manner EMBO reports 2021-11-10 [PMID: 34758190] |
|
|
| Zhao W, Li X, Nian W et al. Ribosome Proteins Represented by RPL27A Mark the Development and Metastasis of Triple-Negative Breast Cancer in Mouse and Human Frontiers in Cell and Developmental Biology 2021-08-23 [PMID: 34497807] (Western Blot, Block/Neutralize) |
Western Blot, Block/Neutralize |
|
| Muzorewa TT, Buerk DG, Jaron D, Barbee KA Coordinated regulation of endothelial calcium signaling and shear stress-induced nitric oxide production by PKC beta and PKC eta. Cellular signalling 2021-08-30 [PMID: 34474112] (WB, Bovine) |
WB |
Bovine |
| Javeed N, Her TK, Brown MR Et al. Pro-inflammatory beta cell small extracellular vesicles induce beta cell failure through activation of the CXCL10/CXCR3 axis in diabetes Cell reports 2021-08-24 [PMID: 34433033] (WB, Mouse) |
WB |
Mouse |
| Lin HW, Lee JY, Chou NL Et al. Phosphorylation of PUF-A/PUM3 on Y259 modulates PUF-A stability and cell proliferation PloS one 2021-08-18 [PMID: 34407138] |
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| Wang H, Chen J, Jandu S Et al. Probing tissue transglutaminase mediated vascular smooth muscle cell aging using a novel transamidation-deficient Tgm2-C277S mouse model Cell death discovery 2021-07-29 [PMID: 34326316] (WB) |
WB |
|
| Jo H, Park H, Choi Y et al. Portal Modulation Effects of Terlipressin on Liver Regeneration and Survival in a Porcine Model Subjected to 90% Hepatectomy Am J Transl Res 2021-07-26 [PMID: 34306332] |
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| Pan CH, Lin YP, Wang JY et al. Preventive Effect and Mechanism of Crossostephium chinense Extract on Balloon Angioplasty-Induced Neointimal Hyperplasia Evidence-based complementary and alternative medicine : eCAM 2021-06-30 [PMID: 34306155] (WB) |
WB |
|
| Behere A, Thornqvist Po, Winberg S Et Al. Visualization of early oligomeric alpha-synuclein pathology and its impact on the dopaminergic system in the (Thy-1)-h[A30P]alpha-syn transgenic mouse model Journal of neuroscience research 2021-07-22 [PMID: 34292621] (WB) |
WB |
|
| Zaganjor E, Yoon H, Spinelli JB et al. SIRT4 is an early regulator of branched-chain amino acid catabolism that promotes adipogenesis Cell reports 2021-07-13 [PMID: 34260923] (WB) |
WB |
|
| Wang H, Poe A, Pak L Et Al. An in situ activity assay for lysyl oxidases Communications biology 2021-07-05 [PMID: 34226627] |
|
|
| Bachmann S, Bell M, Klimek J, Zempel H. Differential Effects of the Six Human TAU Isoforms: Somatic Retention of 2N-TAU and Increased Microtubule Number Induced by 4R-TAU Frontiers in Neuroscience 2021-05-25 [PMID: 34113229] (Immunocytochemistry/ Immunofluorescence) |
Immunocytochemistry/ Immunofluorescence |
|
| Hsieh Y, Lee K, Lei H et al. (Pro)renin Receptor Knockdown Attenuates Liver Fibrosis through Inactivation of ERK/TGF-beta1/SMAD3 Pathway Cellular and Molecular Gastroenterology and Hepatology 2021-06-01 [PMID: 34087453] |
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| Millan C, Prause L, Vallmajo-Martin Q et al. Extracellular Vesicles from 3D Engineered Microtissues Harbor Disease-Related Cargo Absent in EVs from 2D Cultures Advanced healthcare materials 2021-04-22 [PMID: 33890421] |
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| Guldiken N, Argemi J, Gurbuz B et al. Serum transferrin as a biomarker of hepatocyte nuclear factor 4 alpha activity and hepatocyte function in liver diseases BMC medicine 2021-02-17 [PMID: 33593348] |
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|
| Ryder BD, Matlahov I, Bali S et al. Regulatory inter-domain interactions influence Hsp70 recruitment to the DnaJB8 chaperone Nature communications 2021-02-11 [PMID: 33574241] (WB, Human) |
WB |
Human |
| Majumdar U, Manivannan S, Basu M et al. Nitric oxide prevents aortic valve calcification by S-nitrosylation of USP9X to activate NOTCH signaling Science advances 2021-02-01 [PMID: 33547080] (WB, Human) |
WB |
Human |
| Liu T, Xing Y, Fan X et al. Fasting and overfeeding affect the expression of the immunity- or inflammation-related genes in the liver of poultry via endogenous retrovirus (ERV) Poult Sci 2021-02-01 [PMID: 33518151] |
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| Makino T, Izumi K, Hiratsuka K, et al. Anti-proliferative and anti-migratory properties of coffee diterpenes kahweol acetate and cafestol in human renal cancer cells Scientific reports 2021-01-12 [PMID: 33436830] (Human) |
|
Human |
| Fusco P, Mattiuzzo E, Frasson C et al. Verteporfin induces apoptosis and reduces the stem cell-like properties in Neuroblastoma tumour-initiating cells through inhibition of the YAP/TAZ pathway European journal of pharmacology 2020-12-18 [PMID: 33347823] (WB, Human) |
WB |
Human |
| Sanchez-Felipe, L, Vercruysse, T Et al. A single-dose live-attenuated YF17D-vectored SARS-CoV-2 vaccine candidate. Nature 2020-12-01 [PMID: 33260195] (WB, Mouse) |
WB |
Mouse |
| Schlesinger S, Seo S, Pryce B et al. Loss of Smad4 in the scleraxis cell lineage results in postnatal joint contracture Dev Biol 2020-11-25 [PMID: 33248111] |
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Dermit M, Dodel M, Lee FCY et al. Subcellular mRNA Localization Regulates Ribosome Biogenesis in Migrating Cells Dev Cell 2020-11-09 [PMID: 33171110] (WB, Human)
Details:
Used as a control in Western blot along with HEK293T LARP6-knockout cells. |
WB |
Human |
| Pan C, Chen S, Wang J et al. Sclareol ameliorated ERCC1-mediated cisplatin resistance in A549 human lung adenocarcinoma cells and a murine xenograft tumor model by suppressing AKT-GSK3 beta-AP1/Snail and JNK-AP1 pathways Chemico-Biological Interactions 2020-10-01 [PMID: 33164868] (WB) |
WB |
|
| Bou-Gharios J, Assi S, Bahmad HF, Kharroubi H et Al. The potential use of tideglusib as an adjuvant radio-therapeutic treatment for glioblastoma multiforme cancer stem-like cells Pharmacol Rep 2020-11-03 [PMID: 33140310] |
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| Yan S, Khambu B, Chen X et al. Hepatic autophagy deficiency remodels gut microbiota for an adaptive protection via FGF15-FGFR4 signaling Cell Mol Gastroenterol Hepatol 2020-10-22 [PMID: 33127558] (WB, Mouse) |
WB |
Mouse |
| Bahmad HF, Chalhoub RM, Harati H et al. Tideglusib attenuates growth of neuroblastoma cancer stem/progenitor cells in vitro and in vivo by specifically targeting GSK-3 beta Pharmacol Rep 2020-10-08 [PMID: 33030673] |
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WB |
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Details:
Novus GAPDH antibody clone 1D4 was used as a loading control for western blot to look at metabolic enzyme levels in pregnant vs nonpregnant mice |
WB |
Mouse |
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WB |
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WB |
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WB |
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WB |
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Mouse |
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WB |
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Mouse |
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WB |
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WB |
Human |
| Sankowski R, Herring A, Keyvani K et al. The multi-target effects of CNI-1493: convergence of anti-amylodogenic and anti-inflammatory properties in animal models of Alzheimer's disease. Mol. Med. 2016-11-15 [PMID: 27847962] (WB, Mouse) |
WB |
Mouse |
| Lin J, Kumari S et al. RIPK1 counteracts ZBP1-mediated necroptosis to inhibit inflammation. Nature 2016-01-12 [PMID: 27819681] (WB, Mouse) |
WB |
Mouse |
| Yoon YM, Storm KJ, Kamimae-Lanning AN et al. Endogenous DNA Damage Leads to p53-Independent Deficits in Replicative Fitness in Fetal Murine Fancd2(-/-) Hematopoietic Stem and Progenitor Cells. Stem Cell Reports 2016-10-06 [PMID: 27720904] (WB, Mouse) |
WB |
Mouse |
| Palliyaguru DL, Chartoumpekis DV, Wakabayashi N et al. Withaferin A induces Nrf2-dependent protection against liver injury: role of Keap1-independent mechanisms Free Radic Biol Med. 2016-10-03 [PMID: 27717869] (WB, Mouse) |
WB |
Mouse |
| Willi M, Yoo KH, Wang C et al. Differential cytokine sensitivities of STAT5-dependent enhancers rely on Stat5 autoregulation. Nucleic Acids Res. 2016-09-30 [PMID: 27694626] (WB, Mouse) |
WB |
Mouse |
| Drelon C, Berthon A, Sahut-Barnola I et al. PKA inhibits WNT signalling in adrenal cortex zonation and prevents malignant tumour development Nat Commun 2016-09-14 [PMID: 27624192] (WB, Human) |
WB |
Human |
| Sandin L, Bergkvist L, Nath S et al. Beneficial effects of increased lysozyme levels in Alzheimer's disease modelled in Drosophila melanogaster. FEBS J. 2016-08-26 [PMID: 27562772] (WB) |
WB |
|
| Pelham CJ, Drews EM, Agrawal DK. Vitamin D controls resistance artery function through regulation of perivascular adipose tissue hypoxia and inflammation. J Mol Cell Cardiol 2016-09-01 [PMID: 27374117] (Mouse) |
|
Mouse |
| Ochaba J, Monteys AM, O'Rourke JG et al. PIAS1 Regulates Mutant Huntingtin Accumulation and Huntington's Disease-Associated Phenotypes In Vivo. Neuron. 2016-05-04 [PMID: 27146268] (WB, Mouse) |
WB |
Mouse |
| Ding L, Paszkowski-Rogacz M, Winzi M et al. Systems Analyses Reveal Shared and Diverse Attributes of Oct4 Regulation in Pluripotent Cells. Cell Syst 2015-08-26 [PMID: 27135800] (ICC/IF) |
ICC/IF |
|
| Sun Chongxiu, Simon Scott I, Foster Greg A et al. 11,12-Epoxyecosatrienoic acids mitigate endothelial dysfunction associated with estrogen loss and aging: Role of membrane depolarization. Journal of Molecular and Cellular Cardiology 2016-01-01 [PMID: 27079253] (WB, Human) |
WB |
Human |
| Siu MK, Chen WY, Tsai HY et Al. Androgen receptor regulates SRC expression through microRNA-203 Oncotarget 2016-05-03 [PMID: 27028864] (Chromatin Immunoprecipitation) |
Chromatin Immunoprecipitation |
|
| Krapp C, Hotter D, Gawanbacht A et al. Guanylate Binding Protein (GBP) 5 Is an Interferon-Inducible Inhibitor of HIV-1 Infectivity. Cell Host Microbe. 2016-03-15 [PMID: 26996307] (WB, Human) |
WB |
Human |
| Karpel-Massler G, Banu M A et al. Inhibition of deubiquitinases primes glioblastoma cells to apoptosis in vitro and in vivo. Oncotarget 2016-03-15 [PMID: 26872380] (WB, Human) |
WB |
Human |
Kumper S, Mardakheh FK, McCarthy A et al. Rho-associated kinase (ROCK) function is essential for cell cycle progression, senescence and tumorigenesis. Elife. 2016-01-14 [PMID: 26765561] (WB, Mouse)
Details:
GAPDH antibody used as a loading control for WB on mouse embryo fibroblasts (MEFs) from embryos with different genotypes (Figure 1F). |
WB |
Mouse |
| Chen YC, Statt S, Wu R et al. High mobility group box 1-induced epithelial mesenchymal transition in human airway epithelial cells. Sci Rep. 2016-01-07 [PMID: 26739898] (WB, Human) |
WB |
Human |
| Slocum SL, Skoko JJ, Wakabayashi N et al. Keap1/Nrf2 pathway activation leads to a repressed hepatic gluconeogenic and lipogenic program in mice on a high-fat diet. Arch. Biochem. Biophys. 2015-12-14 [PMID: 26701603] (WB, Mouse) |
WB |
Mouse |
| Brai E, Marathe S, Astori S et al. Notch1 Regulates Hippocampal Plasticity Through Interaction with the Reelin Pathway, Glutamatergic Transmission and CREB Signaling. Front Cell Neurosci. 2015-12-04 [PMID: 26635527] (WB, Mouse) |
WB |
Mouse |
| Gokhin DS, Ochala J, Domenighetti AA, Fowler VM. Tropomodulin1 directly controls thin filament length in both wild-type and tropomodulin4-deficient skeletal muscle. Development. 2015-11-19 [PMID: 26586224] (WB, Mouse) |
WB |
Mouse |
| Presutti D, Santini S, Cardinali B et al. MET Gene Amplification and MET Receptor Activation Are Not Sufficient to Predict Efficacy of Combined MET and EGFR Inhibitors in EGFR TKI-Resistant NSCLC Cells. PLoS ONE. 2015-11-20 [PMID: 26580964] (WB, Human) |
WB |
Human |
| Becs G, Zarjou A, Agarwal A et al. Pharmacological induction of ferritin prevents osteoblastic transformation of smooth muscle cells. J Cell Mol Med 2016-02-01 [PMID: 26499096] |
|
|
| DeLuca AP, Whitmore SS, Barnes J et al. Hypomorphic mutations in TRNT1 cause retinitis pigmentosa with erythrocytic microcytosis. Hum Mol Genet 2016-01-01 [PMID: 26494905] (WB) |
WB |
|
| Asghar A, Lajeunesse A, Dulla K et al. Bub1 autophosphorylation feeds back to regulate kinetochore docking and promote localized substrate phosphorylation. Nat Commun 2015-09-24 [PMID: 26399325] (WB) |
WB |
|
| Shang Y, Dong X, Han G et al. The Roles of Tight Junctions and Claudin-1 in the Microbubble-Mediated Ultrasound-Induced Enhancement of Drug Concentrations in Rat Prostate. J. Membr. Biol. 2015-08-20 [PMID: 26289600] (WB, Rat) |
WB |
Rat |
| Antiallodynic effects of alpha lipoic acid in an optimized RR-EAE mouse model of MS-neuropathic pain are accompanied by attenuation of upregulated BDNF-TrkB-ERK signaling in the dorsal horn of the spinal cord. Khan N, Gordon R, Woodruff TM Pharmacol Res Perspect 2005-07-07 [PMID: 26171221] (WB, Mouse) |
WB |
Mouse |
| Ikhapoh IA, Pelham CJ, Agrawal DK. Atherogenic Cytokines Regulate VEGF-A-Induced Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells into Endothelial Cells. Stem Cells Int 2015-01-01 [PMID: 26106428] (WB) |
WB |
|
| Zakrzewicz A, Atanasova S, Padberg W. Monocytic Tissue Transglutaminase in a Rat Model for Reversible Acute Rejection and Chronic Renal Allograft Injury. Mediators of Inflammation. 2015-01-01 [PMID: 26063971] (WB, Rat) |
WB |
Rat |
Boosani CS, Dhar K, Agrawal DK. Down-regulation of hsa-miR-1264 contributes to DNMT1-mediated silencing of SOCS3. Mol. Biol. Rep. 2015-06-06 [PMID: 26047583] (WB, Human)
Details:
GAPDH antibody (clone 1D4) used for WB as a loading control on HCASMCs (human coronary artery smooth muscle) cells. Cells were transfected with hsa-miR-1264 mimic or its inhibitor and treated with or without TNF-alpha and IGF-1 (Fig. 3). |
WB |
Human |
| Serbo JV, Kuo S, Lewis S et al. Patterning of Fibroblast and Matrix Anisotropy within 3D Confinement is Driven by the Cytoskeleton Adv Healthc Mater. 2015-06-01 [PMID: 26033825] |
|
|
| Chelluboina B, Warhekar A, Dillard M et al. Post-transcriptional inactivation of matrix metalloproteinase-12 after focal cerebral ischemia attenuates brain damage. Sci Rep. 2015-05-09 [PMID: 25955565] (WB, Rat) |
WB |
Rat |
| Baskar K, Sur S, Selvaraj V, Agrawal DK Functional constituents of a local serotonergic system, intrinsic to the human coronary artery smooth muscle cells. Mol Biol Rep 2015-08-01 [PMID: 25861735] (Human) |
|
Human |
| Marathe S, Liu S, Brai E et al. Notch signaling in response to excitotoxicity induces neurodegeneration via erroneous cell cycle reentry Cell Death Differ. 2015-03-27 [PMID: 25822340] (IF/IHC, ICC/IF, Mouse) |
IF/IHC, ICC/IF |
Mouse |
| Matos Guedes H, Fonseca-Martins A, Liang y et al. Leishmania amazonensis infection induces PD-L1 expression on dendritic cells in an mTor-dependent manner and impairs Th1 responses in vitro and in vivo bioRxiv Feb 18 2022 (WB, Mouse) |
WB |
Mouse |
| Aboelenain M, Kawahara M, Balboula AZ et al. Status of autophagy, lysosome activity and apoptosis during corpus luteum regression in cattle. J Reprod Dev 2015-06-20 [PMID: 25819401] (WB, Bovine) |
WB |
Bovine |
| Liu YN, Yin J, Barrett B et al. Loss of Androgen Regulated miR-1 Activates SRC and Promotes Prostate Cancer Bone Metastasis Mol. Cell. Biol. 2015-03-23 [PMID: 25802280] (Chemotaxis, WB, Mouse) |
Chemotaxis, WB |
Mouse |
| Zhou Q, Derti A, Ruddy DA et al. A chemical genetics approach for the functional assessment of novel cancer genes Cancer Res. 2015-03-18 [PMID: 25788694] (WB, Human) |
WB |
Human |
| Aubry S, Shin W, Crary JF et al. Assembly and Interrogation of Alzheimers Disease Genetic Networks Reveal Novel Regulators of Progression. PLoS One 2015-01-01 [PMID: 25781952] (WB, Human) |
WB |
Human |
| Bergert M, Erzberger A, Desai RA et al. Force transmission during adhesion-independent migration. Nat. Cell Biol. 2015-04-01 [PMID: 25774834] (WB, Human) |
WB |
Human |
| Zakrzewicz A, Kutlimuratov K, Atanasova S et al. Attenuation of Acute Rat Renal Allograft Rejection by Apolipoprotein E-Mimetic Peptide Transplantation 2015-02-25 [PMID: 25719257] (WB, Human) |
WB |
Human |
| De Boussac H, Maqdasy S, Trousson A et al. Enolase is regulated by Liver X Receptors Steroids. 2015-02-21 [PMID: 25708389] (WB, Mouse) |
WB |
Mouse |
| Kutz JL, Greaney JL, Santhanam L, Alexander LM. Evidence for a functional vasodilatory role for hydrogen sulfide in the human cutaneous microvasculature J. Physiol. (Lond.). 2015-01-30 [PMID: 25639684] (WB, Human, Rat) |
WB |
Human, Rat |
| Li J, Yuan W, Jiang S et al. Prolyl-4-hydroxylase Domain Protein 2 Controls NF-kB/p65 Transactivation and Enhances the Catabolic Effects of Inflammatory Cytokines on cells of the Nucleus Pulposus J. Biol. Chem. 2015-01-29 [PMID: 25635047] (WB, Human, Rat) |
WB |
Human, Rat |
| Izuagie IA, Pelham CJ, Agrawal DK. Synergistic effect of angiotensin II on vascular endothelial growth factor-A-mediated differentiation of bone marrow-derived mesenchymal stem cells into endothelial cells. Stem Cell Res Ther 2015-01-06 [PMID: 25563650] |
|
|
Ye W, Zhou J, Markova DZ et al. Xylosyltransferase-1 Expression Is Refractory to Inhibition by the Inflammatory Cytokines Tumor Necrosis Factor alpha and IL-1b in Nucleus Pulposus Cells: Novel Regulation by AP-1, Sp1, and Sp3. Am. J. Pathol. 2014-12-02 [PMID: 25476526] (WB, Human)
Details:
GAPDH antibody used for WB on lysates of human nucleus pulposus (NP) cells transduced with LV-shC or LV-shSp1 or LV-shSp3 (Figure 6) |
WB |
Human |
Liu Jt, Bain Lj. Arsenic inhibits hedgehog signaling during P19 cell differentiation. Toxicol. Appl. Pharmacol. 2014-12-15 [PMID: 25448440] (WB, Mouse)
Details:
GAPDH antibody used for WB at 1:1000 dilution on lysates of mouse embryonal carcinoma P19 cell line which was induced to differentiate for 5 days while being exposed to 0, 0.25, or 0.5 uM arsenic/ sodium arsenite (Fig 3A and 3C; Fig 6). |
WB |
Mouse |
| Chen WY, Liu SY, Chang YS et Al. MicroRNA-34a regulates WNT/TCF7 signaling and inhibits bone metastasis in Ras-activated prostate cancer Oncotarget 2015-01-01 [PMID: 25436980] |
|
|
| Chang Sh, Chang Wl, Lu Cc, Tarn Wy. Alanine repeats influence protein localization in splicing speckles and paraspeckles. Nucleic Acids Res. 2014-11-20 [PMID: 25414336] |
|
|
| Zhao N, Koenig Sn, Trask Aj et al. mir145 Regulates TGFBR2 Expression and Matrix Synthesis in Vascular Smooth Muscle Cells. Circ. Res. 2014-10-16 [PMID: 25323858] (WB, Mouse) |
WB |
Mouse |
| Peterson Jm, Mart R, Bond Ce. Effect of obesity and exercise on the expression of the novel myokines, Myonectin and Fibronectin type III domain containing 5. PeerJ. 2014-10-07 [PMID: 25289190] (WB, Rat) |
WB |
Rat |
Domingue JC, Ao M, Sarathy J et al. HEK-293 cells expressing the cystic fibrosis transmembrane conductance regulator (CFTR): a model for studying regulation of Cl- transport. Physiol Rep. 2014-09-01 [PMID: 25263207] (WB, Human)
Details:
MRP4 antibody used for WB at 1:750 dilution on cytosolic and total lysate fractions of HEK?CFTR cells as well as T84 cells (positive control). The antibody detected a band of 170 kDa size and the data is shown in Figure 5A - lower panel. |
WB |
Human |
| Chen X, Sadowska GB, Zhang et al. Neutralizing anti-interleukin-1b antibodies modulate fetal blood-brain barrier function after ischemia. Neurobiol. Dis. 2014-09-24 [PMID: 25258170] (WB, Sheep) |
WB |
Sheep |
Wang X, Wang H, Yang H et al. Tumor Necrosis Factor-a- and Interleukin-1b-Dependent Matrix Metalloproteinase-3 Expression in Nucleus Pulposus Cells Requires Cooperative Signaling via Syndecan 4 and Mitogen-Activated Protein Kinase-Nuclear Factor kB Axis: Implications in Inflammatory Disc Disease. Am. J. Pathol. 2014-07-22 [PMID: 25063530] (WB, Rat)
Details:
Glyceraldehyde-3-phosphate dehydrogenase / GAPDH antibody used for WB in TNF-alpha and IL-1 beta treated Rat's Nucleus pulposus/NP cells (Figure 1E, Figure 6A/E); TNF-alpha treated human NP cells transduced with either Sdc4-shRNA (LV-shSDC4) or Ctr-shRNA (LV-shC) (Figure 2C and E); human NP cells transduced with LV co-expressing YFP and either shp65 or shIkk beta (Figure 7C/E); TNF alpha/beta treated rat NP cells (Figure 8D). |
WB |
Rat |
Palsamy P, Bidasee KR, Shinohara T. Selenite cataracts: Activation of endoplasmic reticulum stress and loss of Nrf2/Keap1-dependent stress protection. Biochim. Biophys. Acta 2014-07-02 [PMID: 24997453] (WB, Human)
Details:
GAPDH antibody used for WB on lysates of human lens epithelial cells (HLECs) treated or not with 10 uM sodium selenite for various time periods (Fig 6, Fig 7, Fig 8, Fig 11). |
WB |
Human |
Alidousty C, Rauen T, Hanssen L et al. Calcineurin-mediated YB-1 dephosphorylation regulates CCL5 expression during monocyte differentiation. J. Biol. Chem. 2014-06-19 [PMID: 24947514] (WB, Rat, Mouse, Human)
Details:
Used in WB of GAPDH to ensure equal protein loading of whole cell lysates or cytoplasmatic extracts from Human primary monocytes, THP-1 cells, HEK293 cells, rat mesangial cells (rMCs) and kidney of C57BL/6 mice. |
WB |
Rat, Mouse, Human |
Holmes O, Paturi S, Selkoe DJ, Wolfe MS. Pen-2 Is Essential for gamma-Secretase Complex Stability and Trafficking but Partially Dispensable for Endoproteolysis Biochemistry. 2014-06-30 [PMID: 24941111] (WB, Mouse)
Details:
GAPDH antibody used as loading control in WB on lysates of Pen-2 knockout mouse embryonic fibroblasts/MEFs. Figure 6a - WB on stable cell lines of select Pen-2 mutants - monoclonal cell lines expressing wt, N8A, P27A, N33A, or I53A F-Pen-2; Figure 8 - WB on Pen-2 KO MEFs rescued with mutants of Pen-2 and treated with 8h - 10 uM MG132 ( a peptide aldehyde that effectively block the proteolytic activity of the 26S proteasome complex); Figure 10a - WB on Pen-2 KO MEFs treated with 10 uM MG132 or vehicle. |
WB |
Mouse |
McCord MC, Kullmann PH, He K et al. Syntaxin-binding domain of Kv2.1 is essential for the expression of apoptotic K+ currents. J. Physiol. (Lond.). 2014-06-13 [PMID: 24928958] (WB, Chinese Hamster)
Details:
Figure 3 C - Chinese hamster ovary (CHO) cells expressing syntaxin and Kv2.1 in the absence or presence of peptide C1a441–522 |
WB |
Chinese Hamster |
Chelluboina B, Klopfenstein JD, Pinson DM et al. Stem Cell Treatment After Cerebral Ischemia Regulates the Gene Expression of Apoptotic Molecules Neurochem. Res. 2014-05-31 [PMID: 24879430] (WB, Rat)
Details:
GAPDH antibody used as a loading control for WB on the brain tissues of male Sprague–Dawley rats - sham controls and untreated or hUCBSCs/human umbilical cord blood-derived mesenchymal stem cells - treated ischemic brains (experimental MCAO/middle cerebral artery occlusion model intravenously injected or not with hUCBSCs). WB image in Fig. 4 |
WB |
Rat |
| Bonachea Elizabeth M, Chang Sheng-Wei, Zender Gloria et al. Rare GATA5 sequence variants identified in individuals with bicuspid aortic valve. Pediatr Res. 2014-05-05 [PMID: 24796370] (Human) |
|
Human |
| Palsamy P, Bidasee KR, Ayaki M et al. Methylglyoxal induces endoplasmic reticulum stress and DNA demethylation in the Keap1 promoter of human lens epithelial cells and age-related cataracts. Free Radic. Biol. Med. 2014-04-11 [PMID: 24746615] (WB, Human) |
WB |
Human |
| Wang SH, Cheng CY, Chen CJ et al. Changes in protein expression in testes of L2 strain Taiwan country chickens in response to acute heat stress. Theriogenology 2014-03-18 [PMID: 24725420] (WB, Chicken) |
WB |
Chicken |
| Steppan J, Sikka G, Jandu S et al. Exercise, vascular stiffness, and tissue transglutaminase. J Am Heart Assoc 2014-04-16 [PMID: 24721796] (WB, Rat, Mouse) |
WB |
Rat, Mouse |
| Kensler KH, Slocum SL, Chartoumpekis DV et al. Genetic or Pharmacologic Activation of Nrf2 Signaling Fails to Protect Against Aflatoxin Genotoxicity in Hypersensitive GSTA3 Knockout Mice. Toxicol. Sci. 2014-03-27 [PMID: 24675090] (WB, Mouse) |
WB |
Mouse |
Shah NH, Schulien AJ, Clemens K et al. Cyclin e1 regulates kv2.1 channel phosphorylation and localization in neuronal ischemia. J. Neurosci. 2014-03-19 [PMID: 24647953] (WB, Rat, Human)
Details:
This GAPDH antibody was used as a control in a Western Blot experiment to show an increase in neuronal cyclin E1 expression induced by chemical ischemia. |
WB |
Rat, Human |
| Gokhin DS, Tierney MT, Sui Z, Fowler VM. Calpain-mediated proteolysis of tropomodulin isoforms leads to thin filament elongation in dystrophic skeletal muscle. Mol. Biol. Cell 2014-01-15 [PMID: 24430868] (WB, Mouse) |
WB |
Mouse |
| Grabrucker S, Proepper C, Mangu, et al. The PSD protein ProSAP2/Shank3 displays synapto-nuclear shuttling which is deregulated in a schizophrenia-associated mutation. Exp. Neurol. 2014-03-01 [PMID: 24382453] |
|
|
| Sun J, Liu SZ, Lin Y et al. TGF-beta promotes glioma cell growth via activating Nodal expression through Smad and ERK1/2 pathways. Biochem Biophys Res Commun 2013-12-25 [PMID: 24370825] (WB, Human) |
WB |
Human |
| Brosseau JP, Lucier JF, Nwilati H et al. Tumor microenvironment-associated modifications of alternative splicing. RNA 2014-02-01 [PMID: 24335142] (WB, Human) |
WB |
Human |
| Bohmer M, Sharbati J, Zur Bruegge J et al. Structural Analysis of microRNA-Target Interaction by Sequential Seed Mutagenesis and Stem-Loop 3' RACE. PLoS One. 2013-11-25 [PMID: 24282594] (WB, Human) |
WB |
Human |
| Dickson JR, Kruse C, Montagna DR et al. Alternative polyadenylation and miR-34 family members regulate tau expression. J Neurochem. 2013-12-01 [PMID: 24032460] (WB, IP, Human) |
WB, IP |
Human |
| Premaraj S, Souza I, Premaraj T. Focal adhesion kinase mediates beta-catenin signaling in periodontal ligament cells. Biochem Biophys Res Commun. 2013-10-04 [PMID: 24021281] (WB, Human) |
WB |
Human |
| Jung SM, Jandu S, Steppan J et al. Increased tissue transglutaminase activity contributes to central vascular stiffness in eNOS knockout mice. Am J Physiol Heart Circ Physiol. 2013-09-01 [PMID: 23873798] (WB, Human) |
WB |
Human |
| Hanssen L, Alidousty C, Djudjaj S et al. YB-1 Is an Early and Central Mediator of Bacterial and Sterile Inflammation In Vivo. J Immunol 2013-07-19 [PMID: 23872051] (WB, Mouse) |
WB |
Mouse |
| Roovers D Developing a reactivatable model for for HIV-1 Env expression mimicking latent infection Thesis 2022-01-01 |
|
|
| Lohmann D, Spandl J, Stevanovic A et al. Monoubiquitination of ancient ubiquitous protein 1 promotes lipid droplet clustering. PLoS One 2013-01-01 [PMID: 24039768] (Human) |
|
Human |
| Grotheer V, Goergens M, Fuchs PC et al. The performance of an orthosilicic acid-releasing silica gel fiber fleece in wound healing. Biomaterials. 2013-10-01 [PMID: 23830471] |
|
|
| Chelluboina B, Klopfenstein JD, Gujrati M et al. Temporal Regulation of Apoptotic and Anti-apoptotic Molecules After Middle Cerebral Artery Occlusion Followed by Reperfusion. Mol Neurobiol 2013-06-28 [PMID: 23813097] (WB, Rat) |
WB |
Rat |
| Rostami E, Davidsson J, Gyorgy A et al. The complement terminal pathway is activated in focal penetrating but not in mild diffuse Traumatic Brain Injury. J Neurotrauma 2013-06-28 [PMID: 23808389] (WB, Rat) |
WB |
Rat |
| Potor L, Banyai E, Becs G et al. Atherogenesis May Involve the Prooxidant and Proinflammatory Effects of Ferryl Hemoglobin. Oxid Med Cell Longev 2013-01-01 [PMID: 23766856] (WB, Human) |
WB |
Human |
| Ishimoto T, Cara-Fuentes G, Wang H et al. Serum from minimal change patients in relapse increases CD80 expression in cultured podocytes. Pediatr Nephrol 2013-05-21 [PMID: 23689904] (WB, Human) |
WB |
Human |
| Tian Y, Yuan W, Fujita N et al. Inflammatory Cytokines Associated with Degenerative Disc Disease Control Aggrecanase-1 (ADAMTS-4) Expression in Nucleus Pulposus Cells through MAPK and NF-kB. Am J Pathol 2013-04-17 [PMID: 23602832] (WB, Rat) |
WB |
Rat |
| Wang H, Tian Y, Wang J et al. Inflammatory Cytokines Induce Notch Signaling in Nucleus Pulposus Cells: Implications in Intervertebral Disc Degeneration. J Biol Chem 2013-04-15 [PMID: 23589286] (Human) |
|
Human |
| Bosse K, Hans CP, Zhao N et al. Endothelial nitric oxide signaling regulates Notch1 in aortic valve disease. J Mol Cell Cardiol 2013-04-10 [PMID: 23583836] (WB, Porcine) |
WB |
Porcine |
| Lyssenko NN, Nickel M, Tang C, Phillips MC. Factors controlling nascent high-density lipoprotein particle heterogeneity: ATP-binding cassette transporter A1 activity and cell lipid and apolipoprotein AI availability. FASEB J 2013-07-01 [PMID: 23543682] (Human) |
|
Human |
| Krishnamoorthy V, Donofrio AJ, Martin JL. O-GlcNAcylation of alphaB-crystallin regulates its stress-induced translocation and cytoprotection Mol Cell Biochem 2013-03-30 [PMID: 23543138] (WB, Rat) |
WB |
Rat |
Caldarelli A, Muller JP, Paskowski-Rogacz M et al. A genome-wide RNAi screen identifies proteins modulating aberrant FLT3-ITD signaling. Leukemia 2013-03-19 [PMID: 23508117] (WB, Human)
Details:
Using the PE conjugated version of NBP1-43352, catalog number NBP1-43352PE. |
WB |
Human |
| Lin N, Vu Nguyen T, Shih Y et al. Association of higher transient receptor potential melastatin 8 expression with higher tumor histologic grades, lymph node metastasis, risk factors, and worse survival in patients with head and neck squamous cell carcinoma Journal of Dental Sciences 2023-09-22 |
|
|
| Villaseca S, Leal J, Guajardo J et al. G?i2-Mediated Regulation of Microtubules Dynamics and Rac1 Activity Orchestrates Cranial Neural Crest Cell Migration inXenopus bioRxiv 2023-09-08 (WB, Xenopus) |
WB |
Xenopus |
| Kokubun K, Pankajakshan D, Kim MJ, Agrawal DK. Differentiation of porcine mesenchymal stem cells into epithelial cells as a potential therapeutic application to facilitate epithelial regeneration. J Tissue Eng Regen Med 2013-05-21 [PMID: 23696537] (Porcine) |
|
Porcine |
| Dhar K, Rakesh K, Pankajakshan D, Agrawal DK. SOCS3 promotor hypermethylation and STAT3-NF-kappaB interaction downregulate SOCS3 expression in human coronary artery smooth muscle cells. Am J Physiol Heart Circ Physiol 2013-03-01 [PMID: 23335796] (WB, Human) |
WB |
Human |
| Wang J, Tian Y, Phillips KL et al. Tumor necrosis factor alpha- and interleukin-1beta-dependent induction of CCL3 expression by nucleus pulposus cells promotes macrophage migration through CCR1. Arthritis Rheum 2013-03-01 [PMID: 23233369] (WB, Rat) |
WB |
Rat |
| Wang B, Sun J, Qian S et al. Proliferation and differentiation of osteoblastic cells on silicon-doped TiO2 film deposited by cathodic arc Biomed Pharmacother 2012-09-27 [PMID: 23089479] (WB, Human) |
WB |
Human |
Chen H, Nguyen T, Shih Y et al. Combining microfluidic chip and low-attachment culture devices to isolate oral cancer stem cells Journal of Dental Sciences 2023-10-01 (WB, Human)
Details:
1:10000 dilution |
WB |
Human |
| Jiang P, Du W, Mancuso A et al. Reciprocal regulation of p53 and malic enzymes modulates metabolism and senescence. Nature 2013-01-31 [PMID: 23334421] (WB, Human) |
WB |
Human |
| Youn H, Kim EJ, Um SJ. Zyxin cooperates with PTOV1 to confer retinoic acid resistance by repressing RAR activity. Cancer Lett 2013-01-12 [PMID: 23321499] (WB, Human) |
WB |
Human |
| Akundi RS, Zhi L, Sullivan PG, Bueler H. Shared and Cell Type-Specific Mitochondrial Defects and Metabolic Adaptations in Primary Cells from PINK1-Deficient Mice. Neurodegener Dis 2012-12-29 [PMID: 23295771] |
|
|
| Fang B, Guo HY, Zhang M et al. The Six-Amino Acid Antimicrobial Peptide bLFcin(6) Penetrates Cells and Delivers siRNA FEBS J 2012-12-14 [PMID: 23241223] (WB, Human) |
WB |
Human |
| Gokhin DS, Nowak RB, Kim NE et al. Tmod1 and CP49 Synergize to Control the Fiber Cell Geometry, Transparency, and Mechanical Stiffness of the Mouse Lens PLoS One 2012-01-01 [PMID: 23144950] (WB, Mouse) |
WB |
Mouse |
| Stangis MM Inflammation Induction in the Neurovascular Unit by the S1 Subunit of SARS-CoV-2 Strains Thesis 2023-01-01 (Western Blot, Human) |
Western Blot |
Human |
| Jing SW, Wang YD, Kuroda M et al. HIF-1 alpha Contributes to Hypoxia-induced Invasion and Metastasis of Esophageal Carcinoma via Inhibiting E-cadherin and Promoting MMP-2 Expression Acta Med Okayama 2012-10-01 [PMID: 23093058] (WB, Human) |
WB |
Human |
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WB |
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WB |
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WB |
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Western Blot |
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WB |
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| Azman M Regulation of ribosome biogenesis by KRAS oncogene in Pancreatic Ductal Adenocarcinoma Thesis 2023-01-01 (WB, Mouse) |
WB |
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WB |
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WB |
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WB |
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| Bates RC, Stith BJ, Stevens KE. Chronic central administration of valproic acid: Increased pro-survival phospho-proteins and growth cone associated proteins with no behavioral pathology Pharmacol Biochem Behav 2012-08-31 [PMID: 22960225] (WB, Rat) |
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| Chu M, Koshman Y, Iyengar R et al. Contractile Activity Regulates Inducible Nitric Oxide Synthase Expression and NO(i) Production in Cardiomyocytes via a FAK-Dependent Signaling Pathway J Signal Transduct 2012-01-01 [PMID: 22900166] (WB, Rat) |
WB |
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| Djudjaj S, Chatziantoniou C, Raffetseder U et al. Notch-3 receptor activation drives inflammation and fibrosis following tubulointerstitial kidney injury J Pathol 2012-11-01 [PMID: 22806125] (WB, Rat) |
WB |
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| Pilar AV, Reid-Yu SA, Cooper CA et al. GogB is an anti-inflammatory effector that limits tissue damage during Salmonella infection through interaction with human FBXO22 and Skp1. PLoS Pathog 2012-01-01 [PMID: 22761574] (WB, Mouse) |
WB |
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| Di YP, Zhao J, Harper R. Cigarette Smoke Induces MUC5AC Protein Expression through the Activation of Sp1 J Biol Chem 2012-08-10 [PMID: 22700966] (WB, Human) |
WB |
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| Lange C, Brunswig-Spickenheier B, Eissing L, Scheja L. Platelet lysate suppresses the expression of lipocalin-type prostaglandin D2 synthase that positively controls adipogenic differentiation of human mesenchymal stromal cells Exp Cell Res 2012-06-11 [PMID: 22698646] (WB, Human) |
WB |
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WB |
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WB |
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| Elanchezhian R, Palsamy P, Madson CJ, Mulhern ML, Lynch DW, Troia AM, Usukura J, Shinohara T. Low glucose under hypoxic conditions induces unfolded protein response and produces reactive oxygen species in lens epithelial cells. Cell Death Dis;3. 2012-04-19 [PMID: 22513875] (WB, Human, Rat) |
WB |
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| Matta Benjamin M, Raimondi Giorgio, Rosborough Brian R et al. IL-27 production and STAT3-dependent upregulation of B7-H1 mediate immune regulatory functions of liver plasmacytoid dendritic cells. J Immunol 2012-01-01 [PMID: 22508931] (WB, Mouse) |
WB |
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| Ahn J, Sanz-Moreno V, Marshall CJ. The metastasis gene NEDD9 product acts through integrin beta3 and Src to promote mesenchymal motility and inhibit amoeboid motility. J Cell Sci 2012-04-01 [PMID: 22328516] (Human) |
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| Li M, Chen X, Ye QZ et al. A high-throughput FRET-based assay for determination of Atg4 activity. Autophagy 2012-03-01 [PMID: 22302004] (WB, Human) |
WB |
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| Lassance L, Miedl H, Konya V, Heinemann A, Ebner B, Hackl H, Desoye G, Hiden U. Differential response of arterial and venous endothelial cells to extracellular matrix is modulated by oxygen. Histochem Cell Biol. 2012-02-01 [PMID: 22294260] (WB, Human) |
WB |
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| Thomas AP, Dunn TN, Drayton JB et al. A high calcium diet containing nonfat dry milk reduces weight gain and associated adipose tissue inflammation in diet-induced obese mice when compared to high calcium alone Nutr Metab (Lond) 2012-01-23 [PMID: 22269778] (WB, Mouse) |
WB |
Mouse |
| Hoeffer CA, Sanchez E, Hagerman RJ, Mu Y, Nguyen DV, Wong H, Whelan AM, Zukin RS, Klann E, Tassone F. Altered mTOR signaling and enhanced CYFIP2 expression levels in subjects with fragile X syndrome. Genes Brain Behav doi: 10.1111/j.1601-183X.2012.00768.x. 2012-01-23 [PMID: 22268788] (WB, Human) |
WB |
Human |
| Zhang K, Yuan K, Wu H, Li Q, Wang Y, Chen S, Zhang L, Gu H, Fu R. Identification of potential markers related to neoadjuvant chemotherapy sensitivity of breast cancer by SELDI-TOF MS. Appl Biochem Biotechnol;166(3):753-63. 2012-02-01 [PMID: 22127810] (WB, Human) |
WB |
Human |
| Gokhin DS, Kim NE, Lewis SA et al. Thin filament length correlates with fiber type in human skeletal muscle. American Journal of Physiology. Cell Physiology. 2011-11-09 [PMID: 22075691] (WB, Human) |
WB |
Human |
| Wei Q, Hariharan V, Huang H. Cell-Cell Contact Preserves Cell Viability via Plakoglobin. PLoS ONE;6(10). 2011-01-01 [PMID: 22046445] (WB, Human) |
WB |
Human |
| Rosborough BR, Castellaneta A, Natarajan S et al. Histone deacetylase inhibition facilitates GM-CSF-mediated expansion of myeloid-derived suppressor cells in vitro and in vivo J Leukoc Biol 2012-05-01 [PMID: 22028329] |
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| Fujiwara R, Chen S, Karin M, Tukey RH. Reduced expression of UGT1A1 in intestines of humanized UGT1 mice via inactivation of NF-?B leads to hyperbilirubinemia. Gastroenterology;142(1):109-118 e2. 2012-01-01 [PMID: 21983082] (WB, Mouse) |
WB |
Mouse |
| Veeravalli KK, Ponnala S, Chetty C et al. Integrin ?9[beta]1-mediated cell migration in glioblastoma via SSAT and Kir4.2 potassium channel pathway. Cellular Signalling. 2011-09-17 [PMID: 21946432] (WB, Mouse) |
WB |
Mouse |
| Akundi RS, Zhi L, Bueler H. PINK1 enhances insulin-like growth factor-1-dependent Akt signaling and protection against apoptosis. Neurobiology of Disease. 2011-09-16 [PMID: 21945539] (WB, Mouse) |
WB |
Mouse |
| Lee JW, Liao PC, Young KC, Chang CL, Chen SS, Chang TT, Lai MD, Wang SW. Identification of hnRNPH1, NF45, and C14orf166 as novel host interacting partners of the mature hepatitis C virus core protein. J Proteome Res;10(10):4522-34. 2011-10-07 [PMID: 21823664] (WB, Human) |
WB |
Human |
| Boor P, Celec P et al. The peroxisome proliferator-activated receptor-alpha agonist, BAY PP1, attenuates renal fibrosis in rats. Kidney Int 2011-01-12 [PMID: 21814170] (WB, Mouse) |
WB |
Mouse |
| Martin P, Liu YN, Pierce R et al. Prostate epithelial Pten/TP53 loss leads to transformation of multipotential progenitors and epithelial to mesenchymal transition. Am J Pathol 2011-07-01 [PMID: 21703421] (WB, Mouse) |
WB |
Mouse |
| Nitzsche A, Paszkowski-Rogacz M, Matarese F et al. RAD21 Cooperates with Pluripotency Transcription Factors in the Maintenance of Embryonic Stem Cell Identity. PLoS ONE 201. [PMID: 21589869] (WB, Mouse) |
WB |
Mouse |
Chui JJ, Li MW, Di Girolamo N et al. Iris pigment epithelial cells express a functional lipopolysaccharide receptor complex. Invest Ophthalmol Vis Sci. 2010-05-01 [PMID: 20019359] (WB, Human)
Details:
GAPDH (IMG-5019A-1): WB, human ocular pigment epithelial and normal aqueous cells, Fig 3. |
WB |
Human |
| a DW, Steinmetz AG, Kocinsky HS. NHE3 function and phosphorylation are regulated by a calyculin A-sensitive phosphatase. Am J Physiol Renal Physiol;298(3):F745-53. 2010-03-01 [PMID: 20015946] (WB, Primate, Opossum) |
WB |
Primate, Opossum |
| Bove A. Single cell approaches to study the interaction between normal and transformed cells in epithelial monolayers. Thesis. 2018-01-01 (WB, Canine) |
WB |
Canine |
Jeyasuria P, Subedi K, Suresh A et al. Elevated levels of uterine anti-apoptotic signaling may activate NFKB and potentially confer resistance to caspase 3-mediated apoptotic cell death during pregnancy in mice. Biol Reprod. 2011-08-01 [PMID: 21566000] (WB, Mouse)
Details:
WB: ATF6 (IMG-273) & GAPDH (IMG-5019A-1). Nuclear fractions of mouse uterus, Fig 6. Note: ATF6 (IMG-273) was seen at ~36 kDa, Fig 6. |
WB |
Mouse |
| Tian X, Vroom C, Ghofrani H et al. Phosphodiesterase 10A upregulation contributes to pulmonary vascular remodeling PLoS One 2011-04-11 [PMID: 21494592] (WB, Rat) |
WB |
Rat |
| J-S Roe, H-R Kim, I-Y Hwang, E-J Cho, H-D Youn von Hippel-Lindau protein promotes Skp2 destabilization on DNA damage. Oncogene 2011-09-13 [PMID: 21358672] |
|
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| Tiso M, Tejero J, Basu S et al. Human neuroglobin functions as a redox-regulated nitrite reductase. J Biol Chem 2011-05-20 [PMID: 21296891] (WB) |
WB |
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| Elson-Schwab I, Lorentzen A, Marshall CJ. MicroRNA-200 family members differentially regulate morphological plasticity and mode of melanoma cell invasion. PLoS One 2010-01-01 [PMID: 20957176] (WB, Human) |
WB |
Human |
| Zhao N. Notch Signaling Guides Vascular Smooth Muscle Cell Function. Thesis. 2014-01-01 (WB, Human, Mouse) |
WB |
Human, Mouse |
| Lee C-W, Leu S-JJ, Tzeng R-Y et al. Latent membrane protein 1 of Epstein-Barr virus regulates death-associated protein kinase 1 in lymphoblastoid cell line. Virology. 2011-02-24 [PMID: 21353277] (WB, Human) |
WB |
Human |
| Singaravelu K, Padanilam BJ. p53 target Siva regulates apoptosis in ischemic kidneys. Am J Physiol Renal Physiol;285(11):8013-21;300(5):F1130-41. 2011-05-01 [PMID: 21307125] (ICC/IF, Mouse) |
ICC/IF |
Mouse |
Ritchey ER, Bongini RE, Code KA et al. The pattern of expression of guanine nucleotide-binding protein beta3 in the retina is conserved across vertebrate species. Neuroscience. 2010-09-01 [PMID: 20538044] (WB, IHC-Fr, Chicken)
Details:
GAPDH-Loading Control (IMG-5019A-1). WB & IHC (frozen): P7 wild type and RGE -/- chicken retina, Fig 1B & Fig 1C. |
WB, IHC-Fr |
Chicken |
Swayne LA, Sorbara CD, Bennett SA. Pannexin 2 is expressed by postnatal hippocampal neural progenitors and modulates neuronal commitment. J Biol Chem. 2010-08-06 [PMID: 20529862] (WB)
Details:
WB: Neural stem and progenitor (NPC) cells isolated from postnatal day 3 C57BL/6 mouse hippocampus, Fig 1A. WB: Neuronal cells isolated from 14 day old C57BL/6 mouse neurospheres, Fig 2C & 3C. |
WB |
|
Hirai A, Ohtsuka N, Ikeda T et al. Role of mouse hepatitis virus (MHV) receptor murine CEACAM1 in the resistance of mice to MHV infection: studies of mice with chimeric mCEACAM1a and mCEACAM1b. J Virol. 2010-07-01 [PMID: 20410265] (WB, Mouse)
Details:
WB: The liver, spleen, and ileum of MHV-A59 infected mice, Fig 3A. |
WB |
Mouse |
| Cote MC, Lavoie JR, Houle F et al. Regulation of vascular endothelial growth factor-induced endothelial cell migration by LIM kinase 1-mediated phosphorylation of annexin 1. J Biol Chem. 2010-03-12 [PMID: 20061392] (WB, Human) |
WB |
Human |
| Holden P, Horton WA. Crude subcellular fractionation of cultured mammalian cell lines. BMC Res Notes 2009-12-10 [PMID: 20003239] (WB, Human) |
WB |
Human |
| Zhu ML, Kyprianou N. Role of androgens and the androgen receptor in epithelial-mesenchymal transition and invasion of prostate cancer cells. FASEB J. 2009-11-12 [PMID: 19901020] (WB, Human) |
WB |
Human |
| Costford SR, Bajpeyi S, Pasarica M et al. Skeletal muscle NAMPT is induced by exercise in humans. Am J Physiol Endocrinol Metab 2010-01-01 [PMID: 19887595] (WB, Human) |
WB |
Human |
| Kang YS, Zhao X, Lovaas J et al. Clathrin-independent internalization of normal cellular prion protein in neuroblastoma cells is associated with the Arf6 pathway. J Cell Sci;122(Pt 22):4062-9. 2009-11-15 [PMID: 19843586] (WB, Mouse) |
WB |
Mouse |
| Zhao J, Zhang Y, Ithychanda SS et al. Migfilin interacts with Src and contributes to cell-matrix adhesion-mediated survival signaling. J Biol Chem;284(49):34308-20. 2009-12-04 [PMID: 19833732] (ICC/IF, Human) |
ICC/IF |
Human |
| Kolosionek E, Savai R, Ghofrani HA et al. Expression and activity of phosphodiesterase isoforms during epithelial mesenchymal transition: the role of phosphodiesterase 4. Mol Biol Cell;20(22):4751-65. 2009-11-01 [PMID: 19759179] (WB, Human) |
WB |
Human |
| Fox JT, Shin WK, Caudill MA et al. A UV-responsive internal ribosome entry site enhances serine hydroxymethyltransferase 1 expression for DNA damage repair. J Biol Chem;284(45):31097-108. 2009-11-06 [PMID: 19734144] (WB, Human) |
WB |
Human |
| Fox JT, Stover PJ. Mechanism of the internal ribosome entry site-mediated translation of serine hydroxymethyltransferase 1. J Biol Chem;284(45):31085-96. 2009-11-06 [PMID: 19734143] (WB, Human) |
WB |
Human |
| Jung YW, Kim YT, Kim SW et al. Correlation of human leukocyte antigen-G (HLA-G) expression and disease progression in epithelial ovarian cancer. Reprod Sci;16(11):1103-11. 2009-11-01 [PMID: 19692629] (WB, Human) |
WB |
Human |
| Catalgol B, Ziaja I, Breusing N et al. The Proteasome Is an Integral Part of Solar Ultraviolet A Radiation-induced Gene Expression. J Biol Chem;284(44):30076-30086. 2009-01-01 [PMID: 19690165] (WB, Human) |
WB |
Human |
| Kim JH, Bugaj LJ, Oh YJ et al. Arginase inhibition restores NOS coupling and reverses endothelial dysfunction and vascular stiffness in old rats. J Appl Physiol;107(4):1249-1257. 2009-01-01 [PMID: 19661445] (WB, Rat) |
WB |
Rat |
| Matsui Y, Hadaschik BA, Fazli L et al. Intravesical combination treatment with antisense oligonucleotides targeting heat shock protein-27 HTI-286 as a novel strategy for high-grade bladder cancer. Mol Cancer Ther;8(8):2402-2411. 2009-01-01 [PMID: 19625496] (WB, Mouse) |
WB |
Mouse |
| Guerreiro JR, Lameu C, Oliveira EF et al. Argininosuccinate Synthetase Is a Functional Target for a Snake Venom Anti-hypertensive Peptide: ROLE IN ARGININE NITRIC OXIDE PRODUCTION. J Biol Chem;284(30):20022-20033. 2009-01-01 [PMID: 19491403] (WB, Human) |
WB |
Human |
| Sudarshan S, Sourbier C, Kong H-S et al. Fumarate Hydratase Deficiency in Renal Cancer Induces Glycolytic Addiction Hypoxia-Inducible Transcription Factor 1{alpha} Stabilization by Glucose-Dependent Generation of Reactive Oxygen Species. Mol Cell Biol;29(15):4080-4090. 2009-01-01 [PMID: 19470762] (WB, Human) |
WB |
Human |
| Ding L, Paszkowski-Rogacz M, Nitzsche A et al. A genome-scale RNAi screen for Oct4 modulators defines a role of the Paf1 complex for embryonic stem cell identity. Cell Stem Cell 2009-05-08 [PMID: 19345177] (WB, Mouse) |
WB |
Mouse |
Kang Q, Wang T, Zhang H et al. A Golgi-associated protein 4.1B variant is required for assimilation of proteins in the membrane. J Cell Sci. 2009-04-15 [PMID: 19299464] (IP, Human)
Details:
IP (human bronchial epithelial cells), Fig. 7A. |
IP |
Human |
| Hynynen R, Suchanek M, Spl J et al. OSBP-related protein 2 is a sterol receptor on lipid droplets that regulates the metabolism of neutral lipids. J Lipid Res;50(7):1305-1315. 2009-01-01 [PMID: 19224871] (ICC/IF, Human) |
ICC/IF |
Human |
| Zhao J et al. Interleukin-13-induced MUC5AC is regulated by 15-lipoxygenase 1 pathway in human bronchial epithelial cells. Am J Respir Crit Care Med;179(9):782-90. 2009-05-01 [PMID: 19218191] (WB, Human) |
WB |
Human |
Dong C, Kwas C, Wu L. Transcriptional restriction of human immunodeficiency virus type 1 gene expression in undifferentiated primary monocytes. J Virol. 2009-04-01 [PMID: 19211771] (WB, Human)
Details:
WB (human monocytes), Figs. 5A,C; 7A. |
WB |
Human |
| Devalaraja-Narashimha K, Padanilam BJ. PARP-1 inhibits glycolysis in ischemic kidneys. J Am Soc Nephrol;20(1):95-103. 2009-01-01 [PMID: 19056868] (WB, Mouse) |
WB |
Mouse |
| Woodruff TM et al. The complement factor C5a contributes to pathology in a rat model of amyotrophic lateral sclerosis. J Immunol;181(12):8727-34. 2008-12-15 [PMID: 19050293] (WB, Rat) |
WB |
Rat |
| MacFarlane AJ et al. Mthfd1 is an essential gene in mice and alters biomarkers of impaired one-carbon metabolism. J Biol Chem;284(3):1533-9. 2009-01-16 [PMID: 19033438] (WB, Mouse) |
WB |
Mouse |
| Klein M, Schermuly RT, Ellinghaus P et al. Combined tyrosine and serine/threonine kinase inhibition by sorafenib prevents progression of experimental pulmonary hypertension and myocardial remodeling. Circulation;118(20):2081-90. 2008-11-11 [PMID: 18955668] (WB, Rat) |
WB |
Rat |
| Ezhilarasan Ravesanker, Jadhav Unmesh, Mohanam Indra et al. The hemopexin domain of MMP-9 inhibits angiogenesis and retards the growth of intracranial glioblastoma xenograft in nude mice. International Journal of Cancer 2009-01-01 [PMID: 18942717] (WB, Human) |
WB |
Human |
Singh A, Boldin-Adamsky S, Thimmulappa RK et al. RNAi-mediated silencing of nuclear factor erythroid-2-related factor 2 gene expression in non-small cell lung cancer inhibits tumor growth and increases efficacy of chemotherapy. Cancer Res. 2008-10-01 [PMID: 18829555] (WB, Human)
Details:
WB (human A549 and H460 cells), Fig. 1. |
WB |
Human |
| Schmitt R, Marlier A, Cantley LG. Zag expression during aging suppresses proliferation after kidney injury. J Am Soc Nephrol;19(12):2375-83. 2008-12-01 [PMID: 18815245] (WB, Mouse) |
WB |
Mouse |
| Monteghirfo S et al. Antileukemia effects of xanthohumol in Bcr/Abl-transformed cells involve nuclear factor-kappaB and p53 modulation. Mol Cancer Ther;7(9):2692-702. 2008-09-01 [PMID: 18790751] (WB, Human) |
WB |
Human |
| Vene R et al. Glycogen synthase kinase 3beta regulates cell death induced by synthetic triterpenoids. Cancer Res;68(17):6987-96. 2008-09-01 [PMID: 18757413] (WB, Human) |
WB |
Human |
| Wee S, Wiederschain D, Maira S-M et al. PTEN-deficient cancers depend on PIK3CB. Proc Natl Acad Sci U S A;105(35):13057-62. 2008-09-02 [PMID: 18755892] (WB, Human) |
WB |
Human |
| Smith HW, Marra P, Marshall CJ. uPAR promotes formation of the p130Cas-Crk complex to activate Rac through DOCK180. J Cell Biol;182(4):777-90. 2008-08-25 [PMID: 18725541] (WB, Human) |
WB |
Human |
Alkam T, Nitta A, Mizoguchi H et al. The extensive nitration of neurofilament light chain in the hippocampus is associated with the cognitive impairment induced by amyloid beta in mice. J Pharmacol Exp Ther. 2008-10-01 [PMID: 18621989] (WB, Mouse)
Details:
WB (mouse hippocampal lysates), Fig. 7A. |
WB |
Mouse |
Macartney-Coxson DP, Hood KA, Shi HJ et al. Metastatic susceptibility locus, an 8p hot-spot for tumour progression disrupted in colorectal liver metastases: 13 candidate genes examined at the DNA, mRNA and protein level. BMC Cancer. 2008-07-01 [PMID: 18590575] (WB, Human)
Details:
GAPDH (IMG-5019A). WB: Human colon normal, colon tumour, and liver metastasis, Fig 5. |
WB |
Human |
| Turnquist HR et al. IL-1beta-driven ST2L expression promotes maturation resistance in rapamycin-conditioned dendritic cells. J Immunol;181(1):62-72. 2008-07-01 [PMID: 18566370] (WB, Mouse) |
WB |
Mouse |
| Chetty C, Lakka SS, Bhoopathi P et al. Tissue inhibitor of metalloproteinase 3 suppresses tumor angiogenesis in matrix metalloproteinase 2-down-regulated lung cancer. Cancer Res;68(12):4736-45. 2008-06-15 [PMID: 18559520] (WB, Human) |
WB |
Human |
| Bass-Zubek AE et al. Plakophilin 2: a critical scaffold for PKC alpha that regulates intercellular junction assembly. J Cell Biol;181(4):605-13. 2008-05-19 [PMID: 18474624] (WB, Human) |
WB |
Human |
| Diaz Encarnacion MM et al. Signaling pathways modulated by fish oil in salt-sensitive hypertension. Am J Physiol Renal Physiol;294(6):F1323-35. 2008-06-01 [PMID: 18385269] (WB, Rat) |
WB |
Rat |
| Zhang QS et al. Tempol protects against oxidative damage and delays epithelial tumor onset in Fanconi anemia mice. Cancer Res;68(5):1601-8. 2008-03-01 [PMID: 18316625] (WB, Mouse) |
WB |
Mouse |
| Chen, K, Y Tu et al. PINCH-1 regulates the ERK-Bim pathway and contributes to apoptosis resistance in cancer cells. J Biol Chem. 283(5):2508-17. 2008-02-01 [PMID: 18063582] (WB, Human) |
WB |
Human |
Tsai WJ, McCormick KM, Brazeau DA et al. Estrogen effects on skeletal muscle insulin-like growth factor 1 and myostatin in ovariectomized rats. Exp Biol Med (Maywood). 2007-11-01 [PMID: 17959844] (WB, Rat)
Details:
WB (used as a gel loading control for rat muscle tissue lysates): Figs 6C, 7C, 8C. |
WB |
Rat |
| Limes, S W, P J Rozance et al. Increased insulin sensitivity and maintenance of glucose utilization rates in fetal sheep with placental insufficiency and intrauterine growth restriction. Am J Physiol Endocrinol Metab;293(6):E1716-25. 2007-12-01 [PMID: 17895285] (WB, Sheep) |
WB |
Sheep |
Banerjee PP, Pandey R, Zheng R et al. Cdc42-interacting protein-4 functionally links actin and microtubule networks at the cytolytic NK cell immunological synapse. J Exp Med. 2007-10-01 [PMID: 17785506] (WB)
Details:
WB (Fig 8A): Protein standardization for siRNA studies. |
WB |
|
| Cui, W, E Cuartas et al. CD200 and its receptor, CD200R, modulate bone mass via the differentiation of osteoclasts. Proc Natl Acad Sci U S A;104(36):14436-41. 2007-09-04 [PMID: 17726108] (WB, Mouse) |
WB |
Mouse |
| Woeller CF, Fox JT, Perry C, Stover PJ. A ferritin-responsive internal ribosome entry site regulates folate metabolism. J Biol Chem;282(41):29927-35. 2007-10-12 [PMID: 17702748] (WB, Human) |
WB |
Human |
| Porchia, LM et al. 5-(2-phenanthrenyl)-3-(trifluoromethyl)-1H-pyrazol-1-yl]-phenyl} acetamide (OSU-03012), a celecoxib derivative, directly targets p21-activated kinase. Mol Pharmacol. 72(5):1124-31. Epub 2007 Aug 2. 2007-11-01 [PMID: 17673571] (WB, Human) |
WB |
Human |
Zhou M, Xia L, Wang J. Metformin transport by a newly cloned proton-stimulated organic cation transporter (plasma membrane monoamine transporter) expressed in human intestine. Drug Metab Dispos. 2007-10-01 [PMID: 17600084] (WB, Human)
Details:
Products cited for WB (human small intestine, Fig 6B): 1. GAPDH (IMG-5019A-1, -2) 2. INSTA-BLOT containing human small intenstine tissue lysate (IMB-103, IMB-123). |
WB |
Human |
Mao W, Fukuoka S, Iwai C et al. Cardiomyocyte apoptosis in autoimmune cardiomyopathy: mediated via endoplasmic reticulum stress and exaggerated by norepinephrine. Am J Physiol Heart Circ Physiol. 2007-09-01 [PMID: 17545481] (WB, Rabbit)
Details:
Antibodies cited: 1. ATF6 (IMG-273): WB (Fig 7), analysis of ATF6 in nuclear fraction of rabbit left ventricle myocardium tissue lysates, Authors describe cleaved forms of p90ATF6 in the nucleus: p60ATF6 and p36ATF6. 2. GAPDH (IMG-5019A): WB (Fig 4), GAPD |
WB |
Rabbit |
Blight KJ. Allelic variation in the hepatitis C virus NS4B protein dramatically influences RNA replication. J Virol. 2007-06-01 [PMID: 17360748] (WB, Human)
Details:
Antibodies cited: 1. GAPDH (IMG-5019A-1, IMG-5019A-2) [WB, Fig7, Fig8 (Huh-7.5 human hepatoma cell line)]. |
WB |
Human |
Redman PT, He K, Hartnett KA et al. Apoptotic surge of potassium currents is mediated by p38 phosphorylation of Kv2.1. Proc Natl Acad Sci USA 2007-02-27 [PMID: 17360683] (WB, Rat)
Details:
This GAPDH antibody was used as a loading control in a Western Blot experiment to show injury from oxidation increases S800 phosphorylation in cortical neurons. |
WB |
Rat |
| Block, K et al. NAD(P)H oxidases regulate HIF-2alpha protein expression. J Biol Chem;282(11):8019-26. 2007-03-16 [PMID: 17200123] (WB, Human) |
WB |
Human |
| Xia L, Engel K, Zhou M et al. Membrane localization and pH-dependent transport of a newly cloned organic cation transporter (PMAT) in kidney cells. Am J Physiol Renal Physiol. 2007-02-01 [PMID: 17018840] |
|
|
| Wang G, Wu WW, Pisitkun T et al. Automated quantification tool for high-throughput proteomics using stable isotope labeling and LC-MSn. Anal Chem 2006-08-15 [PMID: 16906720] |
|
|
| Fatma, Sumbul et al. Research Articles: Pluronic L81 enhances triacylglycerol accumulation in the cytosol ingibits chylomicron secretion. 47: 2422-2432, 2006. [PMID: 16894241] (WB, Human) |
WB |
Human |
| Ju, J-S et al. Potentiation of insulin-stimulated glucose transport by the AMP-activated protein kinase. Am J Physiol Cell Physiol;292(1):C564-72. 2007-01-01 [PMID: 16870829] (WB, Mouse) |
WB |
Mouse |
| Kanadia, R N et al. Reversal of RNA missplicing and myotonia after muscleblind overexpression in a mouse poly(CUG) model for myotonic dystrophy. Proc Natl Acad Sci U S A;103(31):11748-53. 2006-08-01 [PMID: 16864772] (WB, Mouse) |
WB |
Mouse |
| Iwatani M, Ikegami K, Kremenska Y et al. Dimethyl sulfoxide has an impact on epigenetic profile in mouse embryoid body. Stem Cells. 2006-11-01 [PMID: 16840553] |
|
|
| Shibata, Yoshimi et al. Heat-killed BCG induces biphasic cyclooxygenase 2+ splenic macrophage formation--role of IL-10 and bone marrow precursors. J Leukoc Biol;80(3):590-8. 2006-09-01 [PMID: 16822852] (WB, Mouse) |
WB |
Mouse |
| Le Boeuf F, Houle F, Sussman M et al. Phosphorylation of focal adhesion kinase (FAK) on Ser732 is induced by rho-dependent kinase and is essential for proline-rich tyrosine kinase-2-mediated phosphorylation of FAK on Tyr407 in response to vascular endothelial growth factor. Mol Biol Cell;17(8):3508-20. 2006-08-01 [PMID: 16760434] (WB, Human) |
WB |
Human |
| Barry, JS et al. Developmental changes in ovine myocardial glucose transporters and insulin signaling following hyperthermia-induced intrauterine fetal growth restriction. Exp Biol Med (Maywood);231(5):566-75. 2006-05-01 [PMID: 16636305] (WB, Sheep) |
WB |
Sheep |
| Azfer, A et al. Activation of endoplasmic reticulum stress response during the development of ischemic heart disease. Am J Physiol Heart Circ Physiol;291(3):H1411-20. 2006-09-01 [PMID: 16617122] (WB, Mouse) |
WB |
Mouse |
| Arthur DB et al. Inhibition of apoptosis by P2Y2 receptor activation: novel pathways for neuronal survival. J Neurosci;26(14):3798-804. 2006-04-05 [PMID: 16597733] (WB, Mouse) |
WB |
Mouse |
| Pisitkun, T et al. High-throughput identification of IMCD proteins using LC-MS/MS. Physiol Genomics;25(2):263-76. 2006-04-13 [PMID: 16449382] (WB, Rat) |
WB |
Rat |
| Jia, Y Takimoto, K Jia Y, Takimoto K. Mitogen-activated protein kinases control cardiac KChIP2 gene expression. Circ Res;98(3):386-93. 2006-02-17 [PMID: 16385079] (WB, Rat) |
WB |
Rat |
| Godsel, LM et al. Desmoplakin assembly dynamics in four dimensions: multiple phases differentially regulated by intermediate filaments and actin. J Cell Biol;171(6):1045-59. 2005-12-19 [PMID: 16365169] (WB, Human) |
WB |
Human |
| Dusek, R L et al. The differentiation-dependent desmosomal cadherin desmoglein 1 is a novel caspase-3 target that regulates apoptosis in keratinocytes. J Biol Chem;281(6):3614-24. 2006-02-10 [PMID: 16286477] (WB, Human) |
WB |
Human |
| Liu X, Kvetnansky R, Serova L et al. Increased susceptibility to transcriptional changes with novel stressor in adrenal medulla of rats exposed to prolonged cold stress. Brain Res Mol Brain Res. 2005-11-18 [PMID: 16169632] |
|
|
| Pendelton, LC et al. Regulation of endothelial argininosuccinate synthase expression and NO production by an upstream open reading frame. J Biol Chem;280(25):24252-60. 2005-06-24 [PMID: 15851478] (WB, Bovine) |
WB |
Bovine |
| Sarkar, S et al. Myocardial cell death and regeneration during progression of cardiac hypertrophy to heart failure. J Biol Chem;279(50):52630-42. 2004-12-10 [PMID: 15385543] (WB, Rat, Human) |
WB |
Rat, Human |
| Gonzales-Cabrera, P et al. Differential regulation of the cell cycle by alpha1-adrenergic receptor subtypes. Endocrinology;145(11):5157-67. 2004-11-01 [PMID: 15297446] (WB, Human, Rat) |
WB |
Human, Rat |
| Moshynska, O et al. Prognostic significance of a short sequence insertion in the MCL-1 promoter in chronic lymphocytic leukemia. J Natl Cancer Inst;96(9):673-82. 2004-05-05 [PMID: 15126604] (WB, Human) |
WB |
Human |
| Akassoglou, K et al. Brain-specific deletion of neuropathy target esterase/swisscheese results in neurodegeneration. Proc Natl Acad Sci U S A;101(14):5075-80. 2004-04-06 [PMID: 15051870] (WB, IF/IHC, ICC/IF, Mouse) |
WB, IF/IHC, ICC/IF |
Mouse |
Ellis RC, Earnhardt JN, Hayes RL et al. Cathepsin B mRNA and protein expression following contusion spinal cord injury in rats. J Neurochem. 2004-02-01 [PMID: 14720218] (WB)
Details:
WB: GAPDH used as a protein loading control (rat spinal cord tissue), Fig. 3a,b,c. |
WB |
|
Fortun J, Dunn WA Jr, Joy S et al. Emerging role for autophagy in the removal of aggresomes in Schwann cells. J Neurosci. 2003-11-19 [PMID: 14627652] (WB, Mouse)
Details:
WB: GAPDH used as a protein loading control (Mouse Schwann cell lysates), Fig. 3F. |
WB |
Mouse |
| Kapadia SB, Brideau-Andersen A, Chisari FV et al. Interference of hepatitis C virus RNA replication by short interfering RNAs. Proc Natl Acad Sci U S A. 2003-02-01 [PMID: 12566571] (WB, ICC/IF, Human) |
WB, ICC/IF |
Human |
|
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![Simple Western Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Simple_Western_21888.jpg)
![Immunocytochemistry Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Immunocytochemistry__Immunofluorescence_17777.jpg)
![Immunohistochemistry Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Immunohistochemistry_23317.jpg)
-(025-ml)_NB300-221_11031.tif)
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![Analysis of cell line lysates probed with mouse mAb to GAPDH (theoretical molecular weight: 36 kDa), NB300-221, dilution 1:2,000: [1] protein standard, [2] HEK293, [3] HeLa, [4] SH-SY5Y, [5] COS1, [6] NIH-3T3, and [7] C6 cells. The GAPDH antibody reveals a single band at ~37 kDa in all cell lines. GAPDH is a house keeping protein, the level of which is relatively unaffected by most experimental manipulations, and, as a result, this antibody has been widely used as a western blotting loading control.](http://images.novusbio.com/fullsize/GAPDH-Antibody-1D4-Western-Blot-NB300-221-img0008.jpg "Analysis of cell line lysates probed with mouse mAb to GAPDH (theoretical molecular weight: 36 kDa), NB300-221, dilution 1:2,000: [1] protein standard, [2] HEK293, [3] HeLa, [4] SH-SY5Y, [5] COS1, [6] NIH-3T3, and [7] C6 cells. The GAPDH antibody reveals a single band at ~37 kDa in all cell lines. GAPDH is a house keeping protein, the level of which is relatively unaffected by most experimental manipulations, and, as a result, this antibody has been widely used as a western blotting loading control.")
![Western Blot: GAPDH Antibody (1D4) [NB300-221] - Notch1, ApoER2 & Dab1 colocalize in hippocampal neurons. (A) Representative image of fluorescent immunolabeling shows that Notch1 is localized in a subset of CA3 neurons, co-expressing Dab1 & ApoER2 (magnified insert). ApoER2 is highly expressed in all CA3 neurons. (B) Fluorescent immunolabeling shows the distribution of Notch1 & Dab1 as compared to Arc/Arg3.1 in CA3 neurons. (B′) Close up of a dendrite showing colocalization of the three proteins (white arrowheads). (C) Western blot analysis on immunoprecipitated samples from whole hippocampal lysate shows that Notch1 displays a strong interaction with Dab1 whereas Notch1 & ApoER2 interaction appears weaker in the preparation (n = 4 independent experiments). (D) Immunolabeling on immunoprecipitated samples from whole cortical lysates show that Notch1 & Dab1 interact. ApoER2 is bound to both Notch1 & Dab1 (n = 3 independent experiments). (E) Co-IP from synaptosomal membrane fractions reveals a stronger interaction between Notch1 & ApoER2, which is reduced in the N1cKO (n = 3 independent pulldowns). In (C–E) GAPDH is used as a loading control for the inputs & to detect contamination in the IP samples. Scale bar in (A) is 50 μm & (B) 25 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26635527), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-221_mouse-monoclonal-gapdh-antibody-1d4-imgenex-img-5019a-310202416205131.jpg "Western Blot: GAPDH Antibody (1D4) [NB300-221] - Notch1, ApoER2 & Dab1 colocalize in hippocampal neurons. (A) Representative image of fluorescent immunolabeling shows that Notch1 is localized in a subset of CA3 neurons, co-expressing Dab1 & ApoER2 (magnified insert). ApoER2 is highly expressed in all CA3 neurons. (B) Fluorescent immunolabeling shows the distribution of Notch1 & Dab1 as compared to Arc/Arg3.1 in CA3 neurons. (B′) Close up of a dendrite showing colocalization of the three proteins (white arrowheads). (C) Western blot analysis on immunoprecipitated samples from whole hippocampal lysate shows that Notch1 displays a strong interaction with Dab1 whereas Notch1 & ApoER2 interaction appears weaker in the preparation (n = 4 independent experiments). (D) Immunolabeling on immunoprecipitated samples from whole cortical lysates show that Notch1 & Dab1 interact. ApoER2 is bound to both Notch1 & Dab1 (n = 3 independent experiments). (E) Co-IP from synaptosomal membrane fractions reveals a stronger interaction between Notch1 & ApoER2, which is reduced in the N1cKO (n = 3 independent pulldowns). In (C–E) GAPDH is used as a loading control for the inputs & to detect contamination in the IP samples. Scale bar in (A) is 50 μm & (B) 25 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26635527), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: GAPDH Antibody (1D4) [NB300-221] - Reduced expression of UNC5A in FancC−/− cells. a Reduced expression of Unc5A in brain cortex of FancC−/− mice compared to wild-type littermates. Western blotting was performed with the indicated antibodies. Each lane represents a different animal (WT: n = 6; FancC−/−: n = 8). b Bar graph represents ratio of Unc5A normalized to tubulin from WT (n = 6) & FancC−/− mice (n = 8). c Bar graph representation of UNC5A expression normalized to Sdha & Tbp from wild-type (WT) & FancC−/−-derived fibroblasts (n = 3). RLU: Relative light units. d–f HEK293T cells were transfected with UNC5AICD, FANCE & FANCC constructs expressing full-length FANCC (in d), FANCC1–306 (in e) or FANCC307–558 (in f). Constructs were transfected at a molecular ratio of 1:1:1 or with 10 times the molar amount of FANCE, FANCC or UNC5AICD as indicated in the figure. The total amount of transfected plasmid was equalized for all strategies with control empty vectors. Representative immunoblots performed with anti-HA (UNC5AICD), anti-FANCC, anti-FANCE or anti-GAPDH antibodies are shown. Arrows indicate appropriate protein bands. Bar graphs represent the mean fold change ± SEM of UNC5A protein levels normalized to GAPDH compared to 1:1:1 transfection controls in at least 4 separate experiments. *p < 0.05; **p < 0.005 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30213274), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-221_mouse-monoclonal-gapdh-antibody-1d4-imgenex-img-5019a-310202416203715.jpg "Western Blot: GAPDH Antibody (1D4) [NB300-221] - Reduced expression of UNC5A in FancC−/− cells. a Reduced expression of Unc5A in brain cortex of FancC−/− mice compared to wild-type littermates. Western blotting was performed with the indicated antibodies. Each lane represents a different animal (WT: n = 6; FancC−/−: n = 8). b Bar graph represents ratio of Unc5A normalized to tubulin from WT (n = 6) & FancC−/− mice (n = 8). c Bar graph representation of UNC5A expression normalized to Sdha & Tbp from wild-type (WT) & FancC−/−-derived fibroblasts (n = 3). RLU: Relative light units. d–f HEK293T cells were transfected with UNC5AICD, FANCE & FANCC constructs expressing full-length FANCC (in d), FANCC1–306 (in e) or FANCC307–558 (in f). Constructs were transfected at a molecular ratio of 1:1:1 or with 10 times the molar amount of FANCE, FANCC or UNC5AICD as indicated in the figure. The total amount of transfected plasmid was equalized for all strategies with control empty vectors. Representative immunoblots performed with anti-HA (UNC5AICD), anti-FANCC, anti-FANCE or anti-GAPDH antibodies are shown. Arrows indicate appropriate protein bands. Bar graphs represent the mean fold change ± SEM of UNC5A protein levels normalized to GAPDH compared to 1:1:1 transfection controls in at least 4 separate experiments. *p < 0.05; **p < 0.005 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30213274), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: GAPDH Antibody (1D4) [NB300-221] - Depletion of RAD21 & other cohesin subunits leads to differentiation of ESCs.(A): qPCR analysis of esiRNA knock-down efficiency for indicated genes (48 h RNAi, n = 3, error bars denote s.d. & *, ** indicate p<0.05 & 0.01 respectively). Transfection of a non-targeting esiRNA (Luc) was used as a control. Numbers adjacent to the gene names indicate independent, non-overlapping esiRNAs transfected. (B): Western Blot analysis of esiRNA knock-down efficiency for indicated genes (48 h RNAi; esi-1 & esi-2 indicate independent, non-overlapping esiRNAs transfected). Transfection of a non-targeting esiRNA (Luc) was used as a control. GAPDH served as a protein loading control. (C): Alkaline phosphatase staining of ESCs, which had been transfected with esiRNAs targeting RAD21, SMC1a & SMC3 (72 h post RNAi) showed a strong differentiation phenotype compared to a control (Luc). Nanog depletion served as a positive control for ESC differentiation. Scale bars correspond to 100 µm. (D): Quantification of alkaline phosphatase staining (n = 3) separated into undifferentiated (green), mixed (yellow) & differentiated colonies (red). (E+F): qPCR validation of expression changes of (E) stem cell maintenance genes & (F) lineage marker genes upon knock-down of RAD21 (two independent esiRNAs) & Luc control (48 h RNAi, n = 3, error bars denote s.d. & *, **, *** indicate p<0.05, 0.01 & 0.001 respectively). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/21589869), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-221_mouse-monoclonal-gapdh-antibody-1d4-imgenex-img-5019a-310202416205163.jpg "Western Blot: GAPDH Antibody (1D4) [NB300-221] - Depletion of RAD21 & other cohesin subunits leads to differentiation of ESCs.(A): qPCR analysis of esiRNA knock-down efficiency for indicated genes (48 h RNAi, n = 3, error bars denote s.d. & *, ** indicate p<0.05 & 0.01 respectively). Transfection of a non-targeting esiRNA (Luc) was used as a control. Numbers adjacent to the gene names indicate independent, non-overlapping esiRNAs transfected. (B): Western Blot analysis of esiRNA knock-down efficiency for indicated genes (48 h RNAi; esi-1 & esi-2 indicate independent, non-overlapping esiRNAs transfected). Transfection of a non-targeting esiRNA (Luc) was used as a control. GAPDH served as a protein loading control. (C): Alkaline phosphatase staining of ESCs, which had been transfected with esiRNAs targeting RAD21, SMC1a & SMC3 (72 h post RNAi) showed a strong differentiation phenotype compared to a control (Luc). Nanog depletion served as a positive control for ESC differentiation. Scale bars correspond to 100 µm. (D): Quantification of alkaline phosphatase staining (n = 3) separated into undifferentiated (green), mixed (yellow) & differentiated colonies (red). (E+F): qPCR validation of expression changes of (E) stem cell maintenance genes & (F) lineage marker genes upon knock-down of RAD21 (two independent esiRNAs) & Luc control (48 h RNAi, n = 3, error bars denote s.d. & *, **, *** indicate p<0.05, 0.01 & 0.001 respectively). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/21589869), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: GAPDH Antibody (1D4) [NB300-221] - Application of the approach to other cell lines. (A) HT1080 cells (2 × 105) & HeLa cells (1 × 105) were plated in the wells of a 12 well plate & the digitonin concentration required for optimal extraction of the cytosol was determined as in Figure1. (B) The remainder of the protocol from Figure 2 was then applied using 100 μg/ml digitonin as the optimal cytosolic extract in each case. An aliquot of each extract was then analyzed by 4-12% SDS PAGE followed by Western blotting & probing with antibodies to GAPDH, BiP & Histone H3 (Used as an alternative nuclear marker due to weakness of the Lamin A signal in HeLa cells). (C) Samples were analyzed by 4-12% SDS PAGE followed by staining with Coomassie blue & then silver staining showing the difference in protein composition of each sample. The bracket alongside the gels marks the position of Histones in the RIPA nuclear extract & the asterisk next to the silver stained gels denotes protein bands unique to the E-RIPA extracts. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/20003239), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-221_mouse-monoclonal-gapdh-antibody-1d4-imgenex-img-5019a-3102024162187.jpg "Western Blot: GAPDH Antibody (1D4) [NB300-221] - Application of the approach to other cell lines. (A) HT1080 cells (2 × 105) & HeLa cells (1 × 105) were plated in the wells of a 12 well plate & the digitonin concentration required for optimal extraction of the cytosol was determined as in Figure1. (B) The remainder of the protocol from Figure 2 was then applied using 100 μg/ml digitonin as the optimal cytosolic extract in each case. An aliquot of each extract was then analyzed by 4-12% SDS PAGE followed by Western blotting & probing with antibodies to GAPDH, BiP & Histone H3 (Used as an alternative nuclear marker due to weakness of the Lamin A signal in HeLa cells). (C) Samples were analyzed by 4-12% SDS PAGE followed by staining with Coomassie blue & then silver staining showing the difference in protein composition of each sample. The bracket alongside the gels marks the position of Histones in the RIPA nuclear extract & the asterisk next to the silver stained gels denotes protein bands unique to the E-RIPA extracts. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/20003239), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: GAPDH Antibody (1D4) [NB300-221] - Role of the TRIM11-USP14 axis in cell growth & apoptosis. a Levels of endogenous TRIM11 in HCT116 cells grown at 43 °C for 0 or 90 min, or at 43 °C for 90 min, & then at 37 °C for 60 or 120 min. b, c Levels of endogenous TRIM11 (b), or Flag-TRIM11, the indicated proteasome subunits, & USP14 (c) in the SN & P fractions of HCT116 cells with & without heat shock at 43 °C for 90 min. d, g TRIM11 overexpression (d) or knockdown (g) HCT116 cells & the corresponding control cells grown under unstressed, heat shock, & heat shock plus recovery conditions were analyzed for K48 polyUb conjugates & caspase-3 activation. e, f, h Apoptosis in TRIM11 overexpression (e, f) or knockdown (h) HCT116 cells, & the corresponding control HCT116 cells, treated with & without heat shock. i, j Caspase-3 activation (i) & apoptosis (j) in HCT116 cells expressing the indicated proteins and grown under normal or heat stress conditions. k–m K48 polyUb conjugates, caspase-3 activation (k), amyloid-like fibrils (l), & apoptosis (m) in HCT116 cells expressing the indicated proteins and grown under normal & heat stress conditions. In e, f, h, j, l, & m, data represent mean ± SEM (n = 3 unless otherwise indicated). *P < 0.05; **P < 0.01; ***P < 0.001. Uncropped blots are presented in Supplementary Fig. 14 Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-03499-z), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-221_mouse-monoclonal-gapdh-antibody-1d4-imgenex-img-5019a-310202416212324.jpg "Western Blot: GAPDH Antibody (1D4) [NB300-221] - Role of the TRIM11-USP14 axis in cell growth & apoptosis. a Levels of endogenous TRIM11 in HCT116 cells grown at 43 °C for 0 or 90 min, or at 43 °C for 90 min, & then at 37 °C for 60 or 120 min. b, c Levels of endogenous TRIM11 (b), or Flag-TRIM11, the indicated proteasome subunits, & USP14 (c) in the SN & P fractions of HCT116 cells with & without heat shock at 43 °C for 90 min. d, g TRIM11 overexpression (d) or knockdown (g) HCT116 cells & the corresponding control cells grown under unstressed, heat shock, & heat shock plus recovery conditions were analyzed for K48 polyUb conjugates & caspase-3 activation. e, f, h Apoptosis in TRIM11 overexpression (e, f) or knockdown (h) HCT116 cells, & the corresponding control HCT116 cells, treated with & without heat shock. i, j Caspase-3 activation (i) & apoptosis (j) in HCT116 cells expressing the indicated proteins and grown under normal or heat stress conditions. k–m K48 polyUb conjugates, caspase-3 activation (k), amyloid-like fibrils (l), & apoptosis (m) in HCT116 cells expressing the indicated proteins and grown under normal & heat stress conditions. In e, f, h, j, l, & m, data represent mean ± SEM (n = 3 unless otherwise indicated). *P < 0.05; **P < 0.01; ***P < 0.001. Uncropped blots are presented in Supplementary Fig. 14 Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-03499-z), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
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