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GAPDH-2 Antibody (2E3-2E10)

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Western Blot: GAPDH-2 Antibody (2E3-2E10) [H00026330-M01] - Analysis of GAPDHS expression in transfected 293T cell line by GAPDS monoclonal antibody (M01), clone 2E3-2E10.Lane 1: GAPDHS transfected lysate(44.5 KDa).Lane ...read more
Immunohistochemistry-Paraffin: GAPDH-2 Antibody (2E3-2E10) [H00026330-M01] - Analysis of monoclonal antibody to GAPDHS on formalin-fixed paraffin-embedded human testis. Antibody concentration 1.5 ug/ml.
Sandwich ELISA: GAPDH-2 Antibody (2E3-2E10) [H00026330-M01] - Detection limit for recombinant GST tagged GAPDHS is approximately 1ng/ml as a capture antibody.

Product Details

Summary
Reactivity Hu, EcSpecies Glossary
Applications WB, ELISA, IHC
Clone
2E3-2E10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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GAPDH-2 Antibody (2E3-2E10) Summary

Immunogen
GAPDS (AAH36373, 1 a.a. ~ 408 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSKRDIVLTNVTVVQLLRQPCPVTRAPPPPEPKAEVEPQPQPEPTPVREEIKPPPPPLPPHPATPPPKMVSVARELTVGINGFGRIGRLVLRACMEKGVKVVAVNDPFIDPEYMVYMFKYDSTHGRYKGSVEFRNGQLVVDNHEISVYQCKEPKQIPWRAVGSPYVVESTGVYLSIQAASDHISAGAQRVVISAPSPDAPMFVMGVNENDYNPGSMNIVSNASCTTNCLAPLAKVIHERFGIVEGLMTTVHSYTATQKTVDGPSRKAWRDGRGAHQNIIPASTGAAKAVTKVIPELKGKLTGMAFRVPTPDVSVVDLTCRLAQPAPYSAIKEAVKAAAKGPMAGILAYTEDEVVSTDFLGDTHSSIFDAKAGIALNDNFVKLISWYDNEYGYSHRVVDLLRYMFSRDK
Specificity
GAPDHS - glyceraldehyde-3-phosphate dehydrogenase, spermatogenic
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
GAPDHS
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
  • Western Blot 1:500
Application Notes
Antibody reactive against recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.
Publications
Read Publications using H00026330-M01.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for GAPDH-2 Antibody (2E3-2E10)

  • EC 1.2.1
  • EC 1.2.1.12
  • GAPD2
  • GAPD2HSD-35
  • GAPDH2
  • GAPDH-2
  • GAPDH-2glyceraldehyde-3-phosphate dehydrogenase, testis-specific
  • GAPDHS
  • GAPDS
  • glyceraldehyde-3-phosphate dehydrogenase, spermatogenic
  • HSD-35
  • Spermatogenic cell-specific glyceraldehyde 3-phosphate dehydrogenase 2
  • Spermatogenic glyceraldehyde-3-phosphate dehydrogenase

Background

This gene encodes a protein belonging to the glyceraldehyde-3-phosphate dehydrogenase family of enzymes that play an important role in carbohydrate metabolism. Like its somatic cell counterpart, this sperm-specific enzyme functions in a nicotinamide adenine dinucleotide-dependent manner to remove hydrogen and add phosphate to glyceraldehyde 3-phosphate to form 1,3-diphosphoglycerate. During spermiogenesis, this enzyme may play an important role in regulating the switch between different energy-producing pathways, and it is required for sperm motility and male fertility.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GAPDH-2 Antibody (H00026330-M01)(12)


Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using H00026330-M01 Applications Species
Kim S, Hata A, Kang H. Down-Regulation of miR-96 by Bone Morphogenetic Protein Signaling is Critical for Vascular Smooth Muscle Cell Phenotype Modulation. J Cell Biochem. 2013-12-04 [PMID: 24375867]
Kang H, Louie J, Weisman A et al. Inhibition of microRNA-302 (miR-302) by bone morphogenetic protein 4 (BMP4) facilitates the BMP signaling pathway. J Biol Chem. 2012-09-17 [PMID: 22988237]
Kuravsky ML, Schmalhausen EV, Pozdnyakova NV, Muronetz VI. Isolation of antibodies against different protein conformations using immunoaffinity chromatography. Anal Biochem. 2012-04-03 [PMID: 22484035]
Kang H, Davis-Dusenbery BN, Nguyen PH et al. Bone morphogenetic protein 4 promotes vascular smooth muscle contractility by activating miR-21, which downregulates expression of the family of Dedicator of Cytokinesis (DOCK) proteins. J Biol Chem. 2011-12-09 [PMID: 22158624]
Davis-Dusenbery BN, Chan MC, Reno KE et al. Down-regulation of KLF4 by MIR-143/145 is critical for modulation of vascular smooth muscle cell phenotype by TGF-{beta} and BMP. J Biol Chem. 2011-06-13 [PMID: 21673106]
Chan MC, Weisman AS, Kang H et al. The amiloride derivative phenamil attenuates pulmonary vascular remodeling by activating NFAT and the BMP signaling pathway. Mol Cell Biol. 2010-12-06 [PMID: 21135135]
Elkina Y, Kuravsky M, Eldarov M et al. Recombinant human sperm-specific glyceraldehyde-3-phosphate dehydrogenase: Structural basis for enhanced stability. Biochim Biophys Acta. 2010-09-15 [PMID: 20833277]
Davis BN, Hilyard AC, Nguyen PH et al. Smad proteins bind a conserved RNA sequence to promote microRNA maturation by Drosha. Mol Cell. 2010-08-13 [PMID: 20705240]
Chan MC, Hilyard AC, Wu C et al. Molecular basis for antagonism between PDGF and the TGFbeta family of signalling pathways by control of miR-24 expression. EMBO J 29(3):559-73. 2010-02-03 [PMID: 20019669]
Davis BN, Hilyard AC, Nguyen PN et al. Induction of microrna-221 by platelet-derived growth factor signaling is critical for modulation of vascular smooth muscle phenotype. J Biol Chem. 2008-12-16 [PMID: 19088079]
Show All 12 Publications.

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Bioinformatics

Gene Symbol GAPDHS
Entrez
OMIM
Uniprot