GAPDH is a 146 kD tetramer glycolytic pathway metabolic enzyme responsible for reversibly phosphorylating glyceraldehyde 3-phosphate. It may have other possible functions in transcriptional activation. GAPDH is highly expressed due to this housekeeping role, and its prevalent expression has allowed its use as an internal loading control – traditionally for mRNA expression comparisons – but also in protein studies. The GAPDH antibody is an excellent standard as reflected in its universal use as such in a wide range of scientific experiments and published literature. The GAPDH antibody was employed to analyze the effect of the tumoricidal protein-lipid complex HAMLET upon cancer cells, where it was found that that HAMLET not only killed cancer cells but also activated an innate immune response in the surrounding tissues via ion channel activation and homeostasis disruption1.
Immunocytochemistry/Immunofluorescence: GAPDH Antibody
Shin’s group used the GAPDH antibody in their studies on prostate cancer (PCa) and the secreted protein acidic and rich in cysteine (SPARC) to profile the modulation of particular tumor metastatic and invasive genes triggered through the integrin beta-1 pathway2. There, they found that SPARC suppressed proliferation and migration in an integrin beta-1-dependent manner. A group from the NYU Medical School determined that the neuropathy target esterase (NTE) has a key physiological role in nervous system preservation and structural development3. Field et al studied the enzyme Mthfs along with the tetrahydrofolate (THF) family of purine synthesis cofactors in mice4. Their use of GAPDH antibody in their mice knockout model system enabled them to demonstrate Mthfs is an essential gene and allowed the identification of several unknown downstream pathways. A recent interesting Nature publication contained data that allowed cancer researchers to establish a connection between p53-mediated metabolism and senescence through use of the GAPDH antibody5.
Novus Biologicals offers GAPDH reagents for your research needs including:
PMIDs
