![Western Blot: Cyclophilin A Antibody [NBP1-30993] - (30 ug of whole cell lysate) A: HepG2 12% SDS PAGE diluted at 1:3000](http://images.novusbio.com/fullsize/Cyclophilin-A-Antibody-Western-Blot-NBP1-30993-img0008.jpg "Western Blot: Cyclophilin A Antibody [NBP1-30993] - (30 ug of whole cell lysate) A: HepG2 12% SDS PAGE diluted at 1:3000")
| Reactivity | Hu, Mu, Rt, Ha, Po, Fe, RM, ShSpecies Glossary |
| Applications | WB, ICC/IF, IHC, KD |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Cyclophilin A. The exact sequence is proprietary. |
| Localization | Cytoplasm |
| Predicted Species | Porcine (100%), Rhesus Macaque (100%), Feline (100%), Sheep (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | PPIA |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 18 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 0.1M Tris-Glycine (pH7), 20% Glycerol |
| Preservative | 0.01% Thimerosal |
| Purity | Antigen Affinity-purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Cyclophilin A Antibody (NBP1-30993)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | PPIA |
![Immunocytochemistry/Immunofluorescence: Cyclophilin A Antibody [NBP1-30993] - Methanol-fixed HeLa, using Cyclophilin A antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/Cyclophilin-A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-30993-img0002.jpg "Immunocytochemistry/Immunofluorescence: Cyclophilin A Antibody [NBP1-30993] - Methanol-fixed HeLa, using Cyclophilin A antibody at 1:200 dilution.")
![Immunohistochemistry-Paraffin: Cyclophilin A Antibody [NBP1-30993] - MDA-MB-468 xenograft, using Cyclophilin A antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Cyclophilin-A-Antibody-Immunohistochemistry-Paraffin-NBP1-30993-img0003.jpg "Immunohistochemistry-Paraffin: Cyclophilin A Antibody [NBP1-30993] - MDA-MB-468 xenograft, using Cyclophilin A antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Western Blot: Cyclophilin A Antibody [NBP1-30993] - Raji whole cell extracts and cytoplasma+membrane and nuclear extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Cyclophilin A antibody at a dilution of 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibod](http://images.novusbio.com/fullsize/Cyclophilin-A-Antibody-Western-Blot-NBP1-30993-img0005.jpg "Western Blot: Cyclophilin A Antibody [NBP1-30993] - Raji whole cell extracts and cytoplasma+membrane and nuclear extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Cyclophilin A antibody at a dilution of 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibod")
![Western Blot: Cyclophilin A Antibody [NBP1-30993] - Analysis. Mouse tissue extracts (50 ug) was separated by 15 % SDS-PAGE, and the membrane was blotted with Cyclophilin A antibody at a dilution of 1:20000.](http://images.novusbio.com/fullsize/Cyclophilin-A-Antibody-Western-Blot-NBP1-30993-img0007.jpg "Western Blot: Cyclophilin A Antibody [NBP1-30993] - Analysis. Mouse tissue extracts (50 ug) was separated by 15 % SDS-PAGE, and the membrane was blotted with Cyclophilin A antibody at a dilution of 1:20000.")
![Knockdown Validated: Cyclophilin A Antibody [NBP1-30993] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Cyclophilin A antibody diluted at 1:20000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Cyclophilin-A-Antibody-Western-Blot-NBP1-30993-img0009.jpg "Knockdown Validated: Cyclophilin A Antibody [NBP1-30993] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Cyclophilin A antibody diluted at 1:20000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: Cyclophilin A Antibody [NBP1-30993] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Cyclophilin A antibody [C1C3] (NBP1-30993) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-30993_rabbit-polyclonal-cyclophilin-a-antibody-91020242021919.jpg "Western Blot: Cyclophilin A Antibody [NBP1-30993] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Cyclophilin A antibody [C1C3] (NBP1-30993) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")