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PPIL3 Antibody

Images

 
Western Blot: PPIL3 Antibody [NBP2-13794] - Analysis in human cell line HEL.
Immunocytochemistry/ Immunofluorescence: PPIL3 Antibody [NBP2-13794] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: PPIL3 Antibody [NBP2-13794] - Staining of human skeletal muscle shows very weak cytoplasmic positivity in myocytes.
Immunohistochemistry-Paraffin: PPIL3 Antibody [NBP2-13794] - Staining of human lymph node shows moderate cytoplasmic positivity in germinal and non-germinal center cells.
Immunohistochemistry-Paraffin: PPIL3 Antibody [NBP2-13794] - Staining of human testis showing moderate cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: PPIL3 Antibody [NBP2-13794] - Staining of human small intestine shows strong cytoplamic postitiity in glandular cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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PPIL3 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to the amino acids: GSQFFITYGKQPHLDMKYTVFGKVIDGLETLDELEKLPVNEKTYRPLNDVHIKDITIHANP
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PPIL3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
PPIL3 Protein (NBP2-13794PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for PPIL3 Antibody

  • Cyclophilin J
  • cyclophilin-like protein 3
  • Cyclophilin-like protein PPIL3
  • CyPJ
  • EC 5.2.1.8
  • peptidyl-prolyl cis-trans isomerase-like 3
  • peptidylprolyl cis-trans isomerase-like protein 3
  • peptidylprolyl isomerase (cyclophilin)-like 3
  • PPIase
  • PPIase-like protein 3
  • Rotamase PPIL3

Background

PPIL3 encodes a member of the cyclophilin family. Cyclophilins catalyze the cis-trans isomerization of peptidylprolyl imide bonds in oligopeptides. They have been proposed to act either as catalysts or as molecular chaperones in protein-folding events. Transcript variants derived from alternative splicing and/or alternative polyadenylation exist; some of these variants encode different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol PPIL3