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Pin1 Antibody (257417) [Unconjugated]

 (12 Publications)
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Western blot shows lysates of Balb/3T3 mouse embryonic fibroblast cell line, U2OS human osteosarcoma cell line, HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, and MDA-MB-453 human breast cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse Pin1 Monoclonal Antibody (Catalog # MAB2294) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Pin1 at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of Balb/3T3 mouse embryonic fibroblast cell line, U2OS human osteosarcoma cell line, HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, and MDA-MB-453 ...read more
Pin1 was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human/Mouse Pin1 Monoclonal Antibody (Catalog # MAB2294) at 8 µg/mL for 3 hours at room temperature. Cells were stained using ...read more
Pin1 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human/Mouse Pin1 Monoclonal Antibody (Catalog # MAB2294) at 25 µg/mL overnight at 4 °C. Tissue was ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IP, ICC/IF
Clone
257417
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB2294-SP
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Pin1 Antibody (257417) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Pin1
Ala2-Glu163
Accession # Q13526
Specificity
Detects human and mouse Pin1 in Western blots.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
PIN1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 8-25 ug/mL
  • Immunoprecipitation 2-3 ug/500 ug cell lysate
  • Western Blot 0.5 ug/mL
Publications
Read Publications using
MAB2294 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Pin1 Antibody (257417) [Unconjugated]

  • dod
  • EC 5.2.1.8
  • peptidylprolyl cis/trans isomerase, NIMA-interacting 1
  • peptidyl-prolyl cis/trans isomerase, NIMA-interacting
  • peptidyl-prolyl cis-trans isomerase NIMA-interacting 1
  • Peptidyl-prolyl cis-trans isomerase Pin1
  • Pin1
  • PPIase Pin1
  • prolyl isomerase
  • protein (peptidyl-prolyl cis/trans isomerase) NIMA-interacting 1
  • protein (peptidylprolyl cis/trans isomerase) NIMA-interacting 1
  • Rotamase Pin1
  • UBL5

Background

Pin1 is a peptidyl-prolyl isomerase (PPI) that targets phosphorylated Ser or Thr residues followed by a Pro (S/T-P). Isomerization of phosphorylated Ser or Thr residues may alter protein confirmation and, subsequently, modify activity. Pin1 is overexpressed in many human breast cancers, and has been shown to modify numerous proteins including p53,NF-kappa B, c-Jun, cyclin D1, and beta -catenin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Pin1 Antibody (MAB2294)(12)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: ICC, IHC-P, Immunoprecipitation, Western Blot.


Filter By Application
ICC
(1)
IHC-P
(1)
Immunoprecipitation
(3)
Western Blot
(4)
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Filter By Species
Human
(4)
Mouse
(3)
All Species
Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using MAB2294 Applications Species
Simone Pelicci, Laura Furia, Pier Giuseppe Pelicci, Mario Faretta Correlative Multi-Modal Microscopy: A Novel Pipeline for Optimizing Fluorescence Microscopy Resolutions in Biological Applications Cells 1/17/2023 [PMID: 36766696]
YT Wang, WY Hsiao, SW Wang The fission yeast Pin1 peptidyl-prolyl isomerase promotes dissociation of Sty1 MAPK from RNA polymerase II and recruits Ssu72 phosphatase to facilitate oxidative stress induced transcription Nucleic Acids Research, 2021-01-25;49(2):805-817. 2021-01-25 [PMID: 33410907] (Western Blot, Human) Western Blot Human
Manuel Daza-Martin, Katarzyna Starowicz, Mohammed Jamshad, Stephanie Tye, George E. Ronson, Hannah L. MacKay et al. Isomerization of BRCA1–BARD1 promotes replication fork protection Nature 7/1/2019 [PMID: 31270457]
X Chen, X Liu, B Deng, M Martinka, Y Zhou, X Lan, Y Cheng Cytoplasmic Pin1 expression is increased in human cutaneous melanoma and predicts poor prognosis Sci Rep, 2018-11-15;8(1):16867. 11/15/2018 [PMID: 30442923]
ZJ Shen, J Hu, V Kashi, YA Bochkov, JE Gern, JS Malter TLR-7 Stress Signaling in Differentiating and Mature Eosinophils Is Mediated by the Prolyl Isomerase Pin1 J. Immunol., 2018-11-05;0(0):. 2018-11-05 [PMID: 30397031] (Immunoprecipitation, Mouse) Immunoprecipitation Mouse
Nancy R. Stallings, Melissa A. O’Neal, Jie Hu, Ege T. Kavalali, Ilya Bezprozvanny, James S. Malter Pin1 mediates A beta 42 -induced dendritic spine loss Science Signaling 3/22/2018 [PMID: 29559586]
F Kruiswijk, S C Hasenfuss, R Sivapatham, M P Baar, D Putavet, K A T Naipal et al. Targeted inhibition of metastatic melanoma through interference with Pin1-FOXM1 signaling Oncogene 4/1/2016 [PMID: 26279295]
Ghosh A, Saminathan H, Kanthasamy A, Anantharam V, Jin H, Sondarva G, Harischandra D, Qian Z, Rana A, Kanthasamy A The peptidyl-prolyl isomerase Pin1 up-regulation and proapoptotic function in dopaminergic neurons: relevance to the pathogenesis of Parkinson disease. J Biol Chem, 2013-06-10;288(30):21955-71. 2013-06-10 [PMID: 23754278] (Western Blot, Human) Western Blot Human
Nishi M, Akutsu H, Masui S A distinct role for Pin1 in the induction and maintenance of pluripotency. J. Biol. Chem., 2011-02-04;286(13):11593-603. 2011-02-04 [PMID: 21296877] (Immunoprecipitation, Human) Immunoprecipitation Human
Brenkman AB, de Keizer PL, van den Broek NJ, van der Groep P, van Diest PJ, van der Horst A, Smits AM, Burgering BM The peptidyl-isomerase Pin1 regulates p27kip1 expression through inhibition of Forkhead box O tumor suppressors. Cancer Res., 2008-09-15;68(18):7597-605. 2008-09-15 [PMID: 18794148] (ICC, IHC-P, Human) ICC, IHC-P Human
Show All 12 Publications.

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Bioinformatics

Gene Symbol PIN1
Uniprot