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GFI-1 Antibody

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Western Blot: GFI-1 Antibody [NBP2-27173] - Analysis of Zinc finger protein Gfi-1 in human HepG2 cell lysate in the 1) absence and 2) presence of immunizing peptide, and 3) mouse RAW cell lysate using this antibody. ...read more
Immunohistochemistry-Paraffin: GFI-1 Antibody [NBP2-27173] - Analysis of Gfi-1 in formalin-fixed, paraffin-embedded human brain tissue using this antibody at 5 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, ChSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

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GFI-1 Antibody Summary

Immunogen
A portion of amino acid 280-330 of human Zinc finger protein Gfi-1 was used as the immunogen.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GFI1
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5ug/ml
  • Western Blot 6-8ug/ml~

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for GFI-1 Antibody

  • GFI1
  • GFI-1
  • growth factor independence-1
  • growth factor independent 1 transcription repressor
  • growth factor independent 1
  • Growth factor independent protein 1
  • Pal-1
  • SCN2
  • Zinc finger protein 163
  • zinc finger protein Gfi-1
  • ZNF163
  • ZNF163FLJ94509

Background

Gfi-1 is a nuclear zinc finger (ZF) transcriptional repressor that plays an important role in hematopoiesis and inner ear development, and has been implicated in lymphomagenesis. The Gfi1 protein expression is regulated by the ubiquitin-proteasome system. It is reported that ubiquitin ligase Triad1 interacts with the DNA-binding domain of Gfi-1. Gfi-1 represses transcription by directly binding to the consensus DNA sequence in the promoters of its target genes. Deficiency of Gfi-1 causes neutropenia and an accumulation of granulomonocytic precursor cells that is reminiscent of a myelodysplastic syndrome in mice.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol GFI1