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GFI-1 Antibodies

We offer GFI-1 Antibodies for use in common research applications: ELISA, Immunocytochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot. Each GFI-1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our GFI-1 Antibodies can be used in a variety of model species: Bovine, Chicken, Human, Mouse, Rat. Use the list below to choose the GFI-1 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our GFI-1 polyclonal antibodies and browse our GFI-1 monoclonal antibody catalog.

Alternate Names for GFI-1 Antibodies

anti-GFI-1 antibody, anti-GFI1 antibody, anti-growth factor independence-1 antibody, anti-growth factor independent 1 antibody, anti-growth factor independent 1 transcription repressor antibody, anti-Growth factor independent protein 1 antibody, anti-SCN2 antibody, anti-Zinc finger protein 163 antibody, anti-zinc finger protein Gfi-1 antibody, anti-ZNF163FLJ94509 antibody

7 Results for "gfi-1" in Primary Antibodies

AF3540

GFI-1 Antibody [Unconjugated]

(5 Publications)

100 μg

AF3540

THP-1 monocytic leukemia cell line was stained with Goat Anti-Human GFI-1SOX2 Affinity Purified Polyclonal Antibody (Catalog # AF3540, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by APC-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

GFI1 interacts with proteins involved in the DDR. a GFI1-Flag fusion protein was immunoprecipitated from 293T cells. Co-precipitated proteins were run on polyacrylamide gel and stained with Coomassie blue. b Peptide sequence of MRE11 with peptides identified through mass spectrometry highlighted and corresponding spectra below. c Peptide sequence of PRMT1 with peptides identified through mass spectrometry highlighted and corresponding spectra below. d Variants of the GFI1-Flag fusion protein were immunoprecipitated from 293T cells. Extracts were separated by SDS–PAGE and blotted for the indicated proteins. e Diagram of the different GFI1 fusion proteins used in d and g. f GFI1-Flag fusion protein was immunoprecipitated in 293T cells in the presence or absence of benzonase. Extracts were separated by SDS–PAGE and blotted for MRE11. g GFI1 KO Jurkat cells were electroporated with plasmids expressing GFI1 variant constructs as shown in e. GFI1 KO and parental control Jurkat cells were used as controls. After 24 h, cells were exposed to 5 Gy IR and allowed to recover for the indicated time. Cells were lysed and analyzed by alkaline Comet assay. Comet tail moment averages are shown. One of three replicate experiments is shown. Error bars represent s.d. h Endogenous GFI1 protein was immunoprecipitated in SupT1 cells. Co-precipitated proteins were separated by SDS–PAGE and blotted for MRE11 and PRMT1. i Endogenous GFI1 protein was immunoprecipitated in Jurkat cells. Co-precipitated proteins were separated by SDS–PAGE and blotted for MRE11 and PRMT1 Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-03817-5), licensed under a CC-BY license. Not internally tested by R&D Systems.

GFI1 activities are independent of DNA damage. a GFI1-Flag fusion protein was immunoprecipitated in 293T cells treated with 5 Gy IR and allowed to recover for the indicated amount of time. Extracts were separated by SDS–PAGE and blotted for the indicated proteins. b SupT1 cells were spread on glass slides 15 min and 1 h after irradiation using a Cytospin, stained for endogenous Gfi1 and gamma -H2AX and visualized for immunofluorescence by confocal microscopy. Control cells stained without primary antibody but with secondary antibodies are shown. c U2OS cells carrying a LacO array and expressing a LacR-Fok1-mCherry endonuclease were transfected with a vector expressing the GFI1-GFP fusion protein. These cells were plated on cover glass, stained for gamma -H2AX and visualized for immunofluorescence by confocal microscopy. d U2OS cells expressing a GFI1-GFP fusion protein were exposed to 405 nm UV micro-irradiation and the recruitment of the GFI1-GFP fusion protein to the site of damage was quantified by confocal microscopy. Average signal intensity is shown with error bars representing s.d. Recruitment of Ku80-mRuby2 fusion protein and GFP protein are shown as controls. Representative images of selected time points are shown on the right. Scale bar represents 10 μm Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-03817-5), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF3540-SP

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NBP2-27173

GFI-1 Antibody

0.1 mg

NBP2-27173

Western Blot: GFI-1 Antibody [NBP2-27173] - Analysis of Zinc finger protein Gfi-1 in human HepG2 cell lysate in the 1) absence and 2) presence of immunizing peptide, and 3) mouse RAW cell lysate using this antibody. goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Immunohistochemistry-Paraffin: GFI-1 Antibody [NBP2-27173] - Analysis of Gfi-1 in formalin-fixed, paraffin-embedded human brain tissue using this antibody at 5 ug/ml.

Reactivity:

Hu, Mu, Rt, Bv, Ch

Species Glossary

Reactivity: Hu, Mu, Rt, Bv, Ch

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-6676A

Alt Catalog# IMG-6676A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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NBP2-45833

GFI-1 Antibody (OTI6E8)

0.1 ml

NBP2-45833

Western Blot: GFI-1 Antibody (6E8) [NBP2-45833] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GFI-1.

Immunohistochemistry: GFI-1 Antibody (6E8) [NBP2-45833] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Immunohistochemistry: GFI-1 Antibody (6E8) [NBP2-45833] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Immunohistochemistry: GFI-1 Antibody (6E8) [NBP2-45833] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Immunohistochemistry: GFI-1 Antibody (6E8) [NBP2-45833] - Analysis of Human Kidney tissue.. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Immunohistochemistry: GFI-1 Antibody (6E8) [NBP2-45833] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-70827AF350
Alexa Fluor 405	NBP2-70827AF405
Alexa Fluor 488	NBP2-70827AF488
Alexa Fluor 532	NBP2-70827AF532
Alexa Fluor 594	NBP2-70827AF594
Alexa Fluor 647	NBP2-70827AF647
Alexa Fluor 700	NBP2-70827AF700
Alexa Fluor 750	NBP2-70827AF750
Allophycocyanin	NBP2-70827APC
Biotin	NBP2-70827B
DyLight 350	NBP2-70827UV
DyLight 405	NBP2-70827V
DyLight 488	NBP2-70827G
DyLight 550	NBP2-70827R
DyLight 594	NBP2-70827DL594
DyLight 650	NBP2-70827C
DyLight 680	NBP2-70827FR
DyLight 755	NBP2-70827IR
FITC	NBP2-70827F
HRP	NBP2-70827H
Janelia Fluor 549	NBP2-70827JF549
Janelia Fluor 646	NBP2-70827JF646
PE	NBP2-70827PE
PerCP	NBP2-70827PCP

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-70827

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NBP2-82227

GFI-1 Antibody

0.1ml

NBP2-82227

Western Blot: GFI-1 Antibody [NBP2-82227] - WB Suggested Anti-GFI1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: HepG2 cell lysate

Western Blot: GFI-1 Antibody [NBP2-82227] - Host: Mouse. Target Name: GFI1. Sample Tissue: Mouse Liver. Antibody Dilution: 1ug/ml

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP2-82230

GFI-1 Antibody

0.1ml

NBP2-82230

Western Blot: GFI-1 Antibody [NBP2-82230] - Host: Rabbit. Target Name: GFI1. Sample Tissue: Human LN18 Whole Cell lysates. Antibody Dilution: 1ug/ml

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP2-82228

GFI-1 Antibody

0.1ml

NBP2-82228

Western Blot: GFI-1 Antibody [NBP2-82228] - WB Suggested Anti-GFI1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: Human Lung

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP2-82229

GFI-1 Antibody

0.1ml

NBP2-82229

Western Blot: GFI-1 Antibody [NBP2-82229] - Host: Rabbit. Target Name: GFI1. Sample Type: Jurkat Whole Cell lysates. Antibody Dilution: 1.0ug/ml

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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