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Flt-3/Flk-2/CD135 Antibody (12B6) - Azide and BSA Free

Images

 
Immunohistochemistry-Paraffin: Flt-3/Flk-2/CD135 Antibody (12B6) [NB110-94155] - Flt-3 antibody staining in mouse lung cancer tissue. IHC-P image submitted by a verified customer review.
Immunohistochemistry: Flt3/CD135 Antibody (12B6) [NB110-94155] - Paraffin kidney and lung sections from LPS treated mice were subjected to IHC using anti Flt3/CD135 Antibody (12B6) RM0021-12B6

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC
Clone
12B6
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

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Flt-3/Flk-2/CD135 Antibody (12B6) - Azide and BSA Free Summary

Immunogen
Mouse Flt-3/Flk-2/CD135 (extracellular domain)
Isotype
IgG2
Clonality
Monoclonal
Host
Rat
Gene
FLT3
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50 - 1:100
  • Western Blot 1:100-1:1000
Reviewed Applications
Read 1 Review rated 5
using
NB110-94155 in the following applications:

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 um filtered solution in PBS. 0.025 mg size is provided in liquid form, PBS
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein G purified
Reconstitution Instructions
Reconstitute with sterilized PBS to a final concentration of 0.2 mg/ml.

Alternate Names for Flt-3/Flk-2/CD135 Antibody (12B6) - Azide and BSA Free

  • CD135 antigen
  • CD135
  • EC 2.7.10
  • fetal liver kinase 2
  • FL cytokine receptor
  • FLK2
  • Flk-2
  • FLT3 receptor tyrosine kinase
  • Flt3
  • Flt-3
  • Fms-like tyrosine kinase 3
  • fms-related tyrosine kinase 3
  • growth factor receptor tyrosine kinase type III
  • Stem cell tyrosine kinase 1
  • STK-1
  • STK1EC 2.7.10.1
  • Tyrosine-protein kinase receptor FLT3

Background

Stem cell tyrosine kinase (STK-1) has been cloned from a CD34+ hematopoietic stem cell enriched library and identified as the human homolog of a previously identified gene of mouse origin designated either Flk-2 or Flt-3. The STK-1 cDNA encodes a protein of 993 amino acids with 85% identity to Flt-3/Flk-2. STK-1 is a member of the type III receptor tyrosine kinase family that includes Kit (steel factor receptor), Fms and PDGF. STK-1 expression in blood and marrow is restricted to CD34+ cells, a population greatly enriched for hematopoietic stem/progenitor cells. STK-1 antiserum recognizes two polypeptides in these cells. The mouse homolog of STK-1, designated Flt-3/ Flk-2, is expressed at high levels in hematopoietic cells and also in neural, gonadal, hepatic and placental tissues. It has been suggested that STK-1 and its murine homolog Flt-3/ Flk-2 may function as growth factor receptors on hematopoietic stem and/or progenitor cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Flt-3/Flk-2/CD135 Antibody (NB110-94155) (0)

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Review for Flt-3/Flk-2/CD135 Antibody (NB110-94155) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB110-94155:
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IHC-P
(1)
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Images Ratings Applications Species Date Details
  5
reviewed by:
Verified Customer
IHC-P Mouse 11/30/2021
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedLung cancer tissue
SpeciesMouse
Lot12B6

Comments

Comments4% formaldehyde fixed paraffin sections were processed in dilutions of ethanol. 1ry antibody dilution was 1:100, 2ry antibody 1:400.

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Secondary Antibodies

 

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Recent Reviews

5
5
1
4
0
3
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2
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1
0

Verified Customer
11/30/2021
Application: IHC-P
Species: Mouse

Bioinformatics

Gene Symbol FLT3
Entrez