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ETFA Antibody

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Genetic Strategies: Western Blot: ETFA Antibody [NBP2-16383] - Wild-type (WT) and ETFA knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with ETFA antibody ...read more
Immunocytochemistry/ Immunofluorescence: ETFA Antibody [NBP2-16383] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: ETFA Antibody [NBP2-16383] - ETFA antibody detects ETFA protein at mitochondria on mouse urinary bladder by immunohistochemical analysis. Sample: Paraffin-embedded mouse urinary bladder. ...read more
Western Blot: ETFA Antibody [NBP2-16383] - ETFA antibody detects ETFA protein by Western blot analysis. A. 30 ug C8D30 whole cell lysate/extract10 % SDS-PAGEETFA antibody dilution: 1:1000.
Western Blot: ETFA Antibody [NBP2-16383] - ETFA antibody detects ETFA protein by Western blot analysis. A. 30 ug PC-12 whole cell lysate/extract10 % SDS-PAGEETFA antibody dilution: 1:1000.
Western Blot: ETFA Antibody [NBP2-16383] - Sample (30 ug of whole cell lysate) A: Hep G2 10% SDS PAGE gel, diluted at 1:1000.
Western Blot: ETFA Antibody [NBP2-16383] - ETFA antibody detects ETFA protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with ETFA antibody ...read more
Western Blot: ETFA Antibody [NBP2-16383] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ETFA antibody diluted at 1:10000. ...read more
Immunohistochemistry-Paraffin: ETFA Antibody [NBP2-16383] - Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using antibody at 1:250 dilution.
Immunohistochemistry-Paraffin: ETFA Antibody [NBP2-16383] - ETFA antibody detects ETFA protein at mitochondria on mouse cervix by immunohistochemical analysis. Sample: Paraffin-embedded mouse cervix. ETFA antibody ...read more
Immunohistochemistry-Paraffin: ETFA Antibody [NBP2-16383] - ETFA antibody detects ETFA protein at mitochondria on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. ETFA antibody ...read more
Immunohistochemistry-Paraffin: ETFA Antibody [NBP2-16383] - ETFA antibody detects ETFA protein at mitochondria on mouse brain stem by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. ETFA antibody ...read more
Immunoprecipitation: ETFA Antibody [NBP2-16383] - Immunoprecipitation of ETFA protein from HepG2 whole cell extracts using 5 ug of ETFA antibody. Western blot analysis was performed using ETFA antibody.
Western Blot: ETFA Antibody [NBP2-16383] - Various tissue extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ETFA antibody (NBP2-16383) diluted at 1:500.
Immunohistochemistry: ETFA Antibody [NBP2-16383] - Immunohistochemical analysis of agarose-embedded zebrafish embryo, using ETFA antibody (NBP2-16383) at 1:100 dilution. (This image was provided courtesy of the ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IP, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

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ETFA Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human ETFA. The exact sequence is proprietary.
Localization
Mitochondrion matrix
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ETFA
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Knockout Validated
  • Western Blot 1:5000-1:20000
Theoretical MW
35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Antigen Affinity-purified

Alternate Names for ETFA Antibody

  • alpha-ETF
  • electron transfer flavoprotein alpha-subunit
  • Electron transfer flavoprotein subunit alpha, mitochondrial
  • electron-transfer-flavoprotein, alpha polypeptide
  • EMA
  • GA2
  • glutaric aciduria II
  • MADD

Background

ETFA participates in catalyzing the initial step of the mitochondrial fatty acid beta-oxidation. It shuttles electrons between primary flavoprotein dehydrogenases and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. Defects in electron-transfer-flavoprotein have been implicated in type II glutaricaciduria in which multiple acyl-CoA dehydrogenase deficiencies result in large excretion of glutaric, lactic, ethylmalonic, butyric, isobutyric, 2-methyl-butyric, and isovaleric acids. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ETFA
Uniprot