![Western Blot: ETFA Antibody [NBP2-16382] - Wild-type (WT) and ETFA knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with ETFA antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ETFA-Antibody-Western-Blot-NBP2-16382-img0011.jpg "Western Blot: ETFA Antibody [NBP2-16382] - Wild-type (WT) and ETFA knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with ETFA antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ETFA. The exact sequence is proprietary. |
| Localization | Mitochondrion matrix |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | ETFA |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 35 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol |
| Preservative | 0.01% Thimerosal |
| Purity | Antigen Affinity-purified |
![Choline Kinase beta [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Choline Kinase beta [Unconjugated]")
Secondary Antibodies |
Isotype Controls |
Research Areas for ETFA Antibody (NBP2-16382)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: ETFA Antibody [NBP2-16382] - Confocal immunofluorescence analysis of methanol-fixed HeLa, using ETFA antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize/ETFA-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16382-img0005.jpg "Immunocytochemistry/Immunofluorescence: ETFA Antibody [NBP2-16382] - Confocal immunofluorescence analysis of methanol-fixed HeLa, using ETFA antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.")
![Immunohistochemistry-Paraffin: ETFA Antibody [NBP2-16382] - ETFA antibody detects ETFA protein at mitochondria on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. ETFA antibody dilution: 1:500.](http://images.novusbio.com/fullsize/ETFA-Antibody-Immunohistochemistry-Paraffin-NBP2-16382-img0010.jpg "Immunohistochemistry-Paraffin: ETFA Antibody [NBP2-16382] - ETFA antibody detects ETFA protein at mitochondria on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. ETFA antibody dilution: 1:500.")
![Western Blot: ETFA Antibody [NBP2-16382] - 30 ug PC-12 whole cell lysate/extract 10 % SDS-PAGE ETFA antibody dilution: 1:1000.](http://images.novusbio.com/fullsize/ETFA-Antibody-Western-Blot-NBP2-16382-img0001.jpg "Western Blot: ETFA Antibody [NBP2-16382] - 30 ug PC-12 whole cell lysate/extract 10 % SDS-PAGE ETFA antibody dilution: 1:1000.")
![Western Blot: ETFA Antibody [NBP2-16382] - Sample (30 ug of whole cell lysate) A: A431 B: HeLa 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/ETFA-Antibody-Western-Blot-NBP2-16382-img0002.jpg "Western Blot: ETFA Antibody [NBP2-16382] - Sample (30 ug of whole cell lysate) A: A431 B: HeLa 10% SDS PAGE gel, diluted at 1:1000.")
![Western Blot: ETFA Antibody [NBP2-16382] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ETFA antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ETFA-Antibody-Western-Blot-NBP2-16382-img0008.jpg "Western Blot: ETFA Antibody [NBP2-16382] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ETFA antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: ETFA Antibody [NBP2-16382] - Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/ETFA-Antibody-Immunohistochemistry-Paraffin-NBP2-16382-img0003.jpg "Immunohistochemistry-Paraffin: ETFA Antibody [NBP2-16382] - Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using antibody at 1:500 dilution.")
![Immunohistochemistry-Paraffin: ETFA Antibody [NBP2-16382] - Immunohistochemical analysis of paraffin-embedded Colon ca, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/ETFA-Antibody-Immunohistochemistry-Paraffin-NBP2-16382-img0004.jpg "Immunohistochemistry-Paraffin: ETFA Antibody [NBP2-16382] - Immunohistochemical analysis of paraffin-embedded Colon ca, using antibody at 1:500 dilution.")
![Immunohistochemistry-Paraffin: ETFA Antibody [NBP2-16382] - ETFA antibody detects ETFA protein at mitochondria on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. ETFA antibody dilution: 1:500.](http://images.novusbio.com/fullsize/ETFA-Antibody-Immunohistochemistry-Paraffin-NBP2-16382-img0007.jpg "Immunohistochemistry-Paraffin: ETFA Antibody [NBP2-16382] - ETFA antibody detects ETFA protein at mitochondria on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. ETFA antibody dilution: 1:500.")
![Immunohistochemistry-Paraffin: ETFA Antibody [NBP2-16382] - ETFA antibody detects ETFA protein at mitochondria on mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. ETFA antibody dilution: 1:500.](http://images.novusbio.com/fullsize/ETFA-Antibody-Immunohistochemistry-Paraffin-NBP2-16382-img0009.jpg "Immunohistochemistry-Paraffin: ETFA Antibody [NBP2-16382] - ETFA antibody detects ETFA protein at mitochondria on mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. ETFA antibody dilution: 1:500.")
![Immunoprecipitation: ETFA Antibody [NBP2-16382] - Immunoprecipitation of ETFA protein from HepG2 whole cell extracts using 5 ug of ETFA antibody. Western blot analysis was performed using ETFA antibody.](http://images.novusbio.com/fullsize/ETFA-Antibody-Immunoprecipitation-NBP2-16382-img0006.jpg "Immunoprecipitation: ETFA Antibody [NBP2-16382] - Immunoprecipitation of ETFA protein from HepG2 whole cell extracts using 5 ug of ETFA antibody. Western blot analysis was performed using ETFA antibody.")
![Immunohistochemistry CEACAM7 Antibody [Unconjugated]](https://images.novusbio.com/images2/CEACAM7_AF4478_Immunohistochemistry_20616.jpg)
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")