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Enolase 1 Antibody (SR1812)

Images

 
Western Blot: Enolase 1 Antibody (SR1812) [NBP3-21624] - Western blot analysis using NBP3-21624 in (1)MCF-7 whole cell lysate; (2)Rat brain tissue lysate.
Immunohistochemistry: Enolase 1 Antibody (SR1812) [NBP3-21624] - Immunohistochemical analysis of paraffin-embedded human colon, using NBP3-21624.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IP
Clone
SR1812
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Enolase 1 Antibody (SR1812) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
A synthesized peptide derived from human Enolase 1 (Uniprot #: P06733 )
Specificity
Detects endogenous levels of total Enolase 1
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
ENO1
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunoprecipitation 1:50
  • Western Blot 1:500-1:2000
Theoretical MW
47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.4, 150mM NaCl, 50% glycerol.
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for Enolase 1 Antibody (SR1812)

  • 2-phospho-D-glycerate hydro-lyase
  • Alpha enolase
  • C-myc promoter-binding protein
  • c-myc promoter-binding protein-1
  • EC 4.2.1
  • EC 4.2.1.11
  • ENO1L1
  • Enolase 1
  • enolase 1, (alpha)
  • MBP-1
  • MBPB1
  • MPB-1
  • MPB1c-myc promoter-binding protein 1
  • MYC promoter-binding protein 1
  • NNE
  • Non-neural enolase
  • Phosphopyruvate hydratase
  • Plasminogen-binding protein
  • PPHalpha enolase like 1
  • tau-crystallin

Background

Enolase 1 encodes one of three enolase isoenzymes found in mammals; it encodes alpha-enolase, a homodimeric soluble enzyme, and also encodes a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter. A pseudogene has been identified that is located on the other arm of the same chromosome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Enolase 1 Antibody (NBP3-21624) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ENO1