Enolase 1 Antibodies
We offer Enolase 1 Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Sandwich ELISA, Simple Western, Western Blot. Each Enolase 1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our Enolase 1 Antibodies can be used in a variety of model species: Bovine, Human, Mouse, Rat, Xenopus. Use the list below to choose the Enolase 1 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our Enolase 1 polyclonal antibodies and browse our Enolase 1 monoclonal antibody catalog.
Alternate Names for Enolase 1 Antibodies
anti-Enolase 1 antibody, anti-ENO1 antibody, anti-2-phospho-D-glycerate hydro-lyase antibody, anti-Alpha enolase antibody, anti-C-myc promoter-binding protein antibody, anti-c-myc promoter-binding protein-1 antibody, anti-EC 4.2.1 antibody, anti-EC 4.2.1.11 antibody, anti-ENO1L1 antibody, anti-enolase 1, (alpha) antibody, anti-MBP-1 antibody, anti-MBPB1 antibody, anti-MPB-1 antibody, anti-MPB1c-myc promoter-binding protein 1 antibody, anti-MYC promoter-binding protein 1 antibody, anti-NNE antibody, anti-Non-neural enolase antibody, anti-Phosphopyruvate hydratase antibody, anti-Plasminogen-binding protein antibody, anti-PPHalpha enolase like 1 antibody, anti-tau-crystallin antibody
14 Results for "enolase-1" in Primary Antibodies
![Western Blot: Enolase 1 Antibody (8G8) [H00002023-M01] - Analysis of ENO1 expression in transfected 293T cell line by ENO1 monoclonal antibody (M01), clone 8G8.Lane 1: ENO1 transfected lysate(47 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Enolase-1-Antibody-8G8-Western-Blot-H00002023-M01-img0015.jpg "Western Blot: Enolase 1 Antibody (8G8) [H00002023-M01] - Analysis of ENO1 expression in transfected 293T cell line by ENO1 monoclonal antibody (M01), clone 8G8.Lane 1: ENO1 transfected lysate(47 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: Enolase 1 Antibody (8G8) [H00002023-M01] - Analysis of monoclonal antibody to ENO1 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Enolase-1-Antibody-8G8-Immunocytochemistry-Immunofluorescence-H00002023-M01-img0008.jpg)
![Immunohistochemistry-Paraffin: Enolase 1 Antibody (8G8) [H00002023-M01] - Analysis of monoclonal antibody to ENO1 on formalin-fixed paraffin-embedded human lymphoma tissue. Antibody concentration 1 ug/ml.](http://images.novusbio.com/fullsize/Enolase-1-Antibody-8G8-Immunohistochemistry-Paraffin-H00002023-M01-img0010.jpg)
![Western Blot: Enolase 1 Antibody (8G8) [H00002023-M01] - Analysis of ENO1 transfected lysate using anti-ENO1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ENO1 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/Enolase-1-Antibody-8G8-Western-Blot-H00002023-M01-img0011.jpg)
![Western Blot: Enolase 1 Antibody (8G8) [H00002023-M01] - ENO1 monoclonal antibody (M01), clone 8G8 Analysis of ENO1 expression in MCF-7.](http://images.novusbio.com/fullsize/Enolase-1-Antibody-8G8-Western-Blot-H00002023-M01-img0013.jpg)
![Western Blot: Enolase 1 Antibody (8G8) [H00002023-M01] - ENO1 monoclonal antibody (M01), clone 8G8. Analysis of ENO1 expression in human colon.](http://images.novusbio.com/fullsize/Enolase-1-Antibody-8G8-Western-Blot-H00002023-M01-img0014.jpg)
![ELISA: Enolase 1 Antibody (8G8) [H00002023-M01] - Detection limit for recombinant GST tagged ENO1 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Enolase-1-Antibody-8G8-ELISA-H00002023-M01-img0016.jpg)
![Western Blot: Enolase 1 Antibody (8G8) [H00002023-M01] - LHPP suppresses aerobic glycolysis. Protein expression levels of Enolase 1 in LHPP-overexpressing or LHPP-knockdown MGC-803 cells and control cells on western blot. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35568711/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/h00002023-m01_mouse-monoclonal-enolase-1-antibody-8g8-25520231539522.jpg)
![Western Blot: Enolase 1 Antibody (8G8) [H00002023-M01] - LHPP suppresses aerobic glycolysis.A Relative mRNA expression levels of glycolytic genes & glutamine transporters in LHPP-overexpressing or LHPP-knockdown MGC-803 cells & control cells by qPCR. B Protein expression levels of glycolytic genes & glutamine transporters in LHPP-overexpressing or LHPP-knockdown MGC-803 cells & control cells on western blot. C IHC staining of LHPP & HIF1A in TMAs & their correlation. Scale bars = 100 μm. D Oxygen consumption rate & extracellular acidification rate of LHPP-overexpressing or LHPP-knockdown MGC-803 cells & control cells were measured using the Seahorse Bioscience XF96 analyser. E Human phosphokinase microarray assay analysis of the conditioned medium from stably transfected HGC-27 cells. A summary of the relative signal intensities of the indicated proteins is shown. G, H IHC staining of LHPP & p-GSK3b in TMAs & their correlation. Scale bars = 200 μm. F Combined LHPP & GSK3b by co-immunoprecipitation. I Protein expression levels of the Wnt pathway in LHPP-overexpressing or LHPP-knockdown MGC-803 cells & control cells on western blot. Data were analyzed using the Wilcoxon test. *P < 0.05, **P < 0.01, ***P < 0.001, KEGG Kyoto encyclopaedia of genes & genomes, GO gene ontology, qPCR quantitative polymerase chain reaction, IHC immunohistochemical, TMA tissue microarray. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35568711), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00002023-m01_mouse-monoclonal-enolase-1-antibody-8g8-310202415482282.jpg)
![Western Blot: Enolase 1 Antibody (253) [NBP2-25147] - Analysis of different cell lysates using mouse mAb to alpha-enolase, NBP2-25147, dilution 1:10,000 in green: [1] protein standard (red), [2] NIH-3T3 l, [3] C6, [4] HEK293, [5] HeLa, and [6] SH-SY5Y cells. A strong single band at 47kDa corresponds to the alpha-enolase protein.](http://images.novusbio.com/fullsize/Enolase-1-Antibody-253-Western-Blot-NBP2-25147-img0008.jpg "Western Blot: Enolase 1 Antibody (253) [NBP2-25147] - Analysis of different cell lysates using mouse mAb to alpha-enolase, NBP2-25147, dilution 1:10,000 in green: [1] protein standard (red), [2] NIH-3T3 l, [3] C6, [4] HEK293, [5] HeLa, and [6] SH-SY5Y cells. A strong single band at 47kDa corresponds to the alpha-enolase protein.")
![Immunocytochemistry/Immunofluorescence: Enolase 1 Antibody (253) [NBP2-25147] - Analysis of HeLa cells stained with mouse mAb to alpha-enolase, NBP2-25147, dilution 1:500 in green and costained with chicken pAb to HSP60, dilution 1:5,000, in red. The blue is DAPI staining of nuclear DNA. NBP2-25147 antibody reveals strong cytoplasmic staining.](http://images.novusbio.com/fullsize/Enolase-1-Antibody-253-Immunocytochemistry-Immunofluorescence-NBP2-25147-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Enolase 1 Antibody (253) [NBP2-25147] - Rat 3T3 cells stained with NBP2-25147 (green) and counterstained with chicken polyclonal antibody to Vimentin NB300-223 (red) and DNA (blue). NBP2-25147 reveals strong cytoplasmic staining, while the Vimentin antibody reveals cytoplasmic intermediate filaments.](http://images.novusbio.com/fullsize/Enolase-1-Antibody-253-Immunocytochemistry-Immunofluorescence-NBP2-25147-img0003.jpg)
![Simple Western: Enolase 1 Antibody (253) [NBP2-25147] - Simple Western lane view shows a specific band for Enolase 1 in 0.5 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Enolase-1-Antibody-253-Simple-Western-NBP2-25147-img0006.jpg)
![Western Blot: Enolase 1 Antibody [NBP1-31324] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ENO1 antibody [N3C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Enolase-1-Antibody-Western-Blot-NBP1-31324-img0012.jpg "Western Blot: Enolase 1 Antibody [NBP1-31324] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ENO1 antibody [N3C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: Enolase 1 Antibody [NBP1-31324] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ENO1 protein stained by ENO1 antibody [N3C3] diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] diluted at 1:10000. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Enolase-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31324-img0008.jpg)
![Immunohistochemistry-Paraffin: Enolase 1 Antibody [NBP1-31324] - Paraffin-embedded gastric tissue, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/Enolase-1-Antibody-Immunohistochemistry-Paraffin-NBP1-31324-img0003.jpg)
![Western Blot: Enolase 1 Antibody [NBP1-31324] - Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Enolase-1-Antibody-Western-Blot-NBP1-31324-img0004.jpg)
![Western Blot: Enolase 1 Antibody [NBP1-31324] - Sample (30 ug of whole cell lysate) A: A431 B: H1299 10% SDS PAGE, antibody diluted at 1:3000.](http://images.novusbio.com/fullsize/Enolase-1-Antibody-Western-Blot-NBP1-31324-img0005.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Enolase 1 Antibody [NBP1-31324] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ENO1 antibody [N3C3].](http://images.novusbio.com/fullsize/Enolase-1-Antibody-Western-Blot-NBP1-31324-img0007.jpg)
![Western Blot: Enolase 1 Antibody [NBP1-31324] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with ENO1 antibody [N3C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/Enolase-1-Antibody-Western-Blot-NBP1-31324-img0010.jpg)
![Western Blot: Enolase 1 Antibody [NBP1-31324] - Sample (30 ug of whole cell lysate) A: zebrafish eye 10% SDS PAGE NBP1-31324 diluted at 1:1000](http://images.novusbio.com/fullsize/nbp1-31324_rabbit-polyclonal-enolase-1-antibody-910202420134090.jpg)
![Immunohistochemistry: Enolase 1 Antibody [NBP1-31324] - Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using Enolase 1 antibody [N3C3] (NBP1-31324) at 1:300 dilution.](http://images.novusbio.com/fullsize/nbp1-31324_rabbit-polyclonal-enolase-1-antibody-91020242041252.jpg)
![Immunohistochemistry: Enolase 1 Antibody [NBP1-31324] - Enolase 1 antibody [N3C3] detects Eno1 protein on zebrafish by whole mount immunohistochemical analysis. Sample: 2 days-post-fertilization zebrafish embryo. Enolase 1 antibody [N3C3] (NBP1-31324) dilution: 1:100.](http://images.novusbio.com/fullsize/nbp1-31324_rabbit-polyclonal-enolase-1-antibody-910202420134054.jpg)
![Western Blot: Enolase 1 Antibody [NBP2-16337] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Enolase 1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Enolase-1-Antibody-Western-Blot-NBP2-16337-img0007.jpg "Western Blot: Enolase 1 Antibody [NBP2-16337] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Enolase 1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: Enolase 1 Antibody [NBP2-16337] - Immunofluorescence analysis of methanol-fixed HCT116, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Enolase-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16337-img0004.jpg)
![Immunohistochemistry-Paraffin: Enolase 1 Antibody [NBP2-16337] - Immunohistochemical analysis of paraffin-embedded U87 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Enolase-1-Antibody-Immunohistochemistry-Paraffin-NBP2-16337-img0002.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Enolase 1 Antibody [NBP2-16337] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ENO1 antibody.](http://images.novusbio.com/fullsize/Enolase-1-Antibody-Western-Blot-NBP2-16337-img0005.jpg)
![Western Blot: Enolase 1 Antibody [NBP2-16337] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Enolase 1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Enolase-1-Antibody-Western-Blot-NBP2-16337-img0006.jpg)
![Immunoprecipitation: Enolase 1 Antibody [NBP2-16337] - Sample: 1000 ug HeLa whole cell lysate/extract A. 40 ug HeLa whole cell lysate/extract B. Control with 2. 5 ug of preimmune rabbit IgG C. Immunoprecipitation of ENO1 protein by 2. 5 ug of ENO1 antibody 10% SDS-PAGE The immunoprecipitated ENO1 protein was detected by ENO1 antibody diluted at 1:1000. anti-rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/Enolase-1-Antibody-Immunoprecipitation-NBP2-16337-img0003.jpg)
![Western Blot: Enolase 1 Antibody [NBP3-03484] - Analysis of extracts of various cell lines, using Enolase 1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/Enolase-1-Antibody-Western-Blot-NBP3-03484-img0006.jpg "Western Blot: Enolase 1 Antibody [NBP3-03484] - Analysis of extracts of various cell lines, using Enolase 1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.")
![Immunocytochemistry/Immunofluorescence: Enolase 1 Antibody [NBP3-03484] - Analysis of U-2 OS cells using Enolase 1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Enolase-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-03484-img0002.jpg)
![Immunohistochemistry-Paraffin: Enolase 1 Antibody [NBP3-03484] - Analysis of 200ug extracts of HeLa cells using Enolase 1 antibody . Western blot was performed from the immunoprecipitate using this antibody.](http://images.novusbio.com/fullsize/Enolase-1-Antibody-Immunohistochemistry-Paraffin-NBP3-03484-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Enolase 1 Antibody [NBP3-03484] - Analysis of NIH-3T3 cells using Enolase 1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Enolase-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-03484-img0001.jpg)
![Immunohistochemistry-Paraffin: Enolase 1 Antibody [NBP3-03484] - Paraffin-embedded human colon carcinoma using Enolase 1 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/Enolase-1-Antibody-Immunohistochemistry-Paraffin-NBP3-03484-img0003.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Enolase 1 Antibody [NBP3-03484] - Analysis of extracts from normal (control) and ENO1 knockout (KO) HeLa cells, using Enolase 1 antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/Enolase-1-Antibody-Knockout-Validated-NBP3-03484-img0005.jpg)
![Western Blot: Enolase 1 Antibody (1G7) [NBP2-59457] - The recombinant Enolase 1 protein [NBP1-30262] (50ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human [NBP2-59457] (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: ENO1 (Alpha-enolase) recombinant protein.](http://images.novusbio.com/fullsize/Enolase-1-Antibody-1G7-Western-Blot-NBP2-59457-img0006.jpg "Western Blot: Enolase 1 Antibody (1G7) [NBP2-59457] - The recombinant Enolase 1 protein [NBP1-30262] (50ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human [NBP2-59457] (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: ENO1 (Alpha-enolase) recombinant protein.")
![Immunocytochemistry/Immunofluorescence: Enolase 1 Antibody (1G7) [NBP2-59457] - Analysis of ENO1, 2 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ENO1, 2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/Enolase-1-Antibody-1G7-Immunocytochemistry-Immunofluorescence-NBP2-59457-img0002.jpg)
![Flow Cytometry: Enolase 1 Antibody (1G7) [NBP2-59457] - Analysis of ENO1, 2 in LNCap cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).](http://images.novusbio.com/fullsize/Enolase-1-Antibody-1G7-Flow-Cytometry-NBP2-59457-img0003.jpg)
![Western Blot: Enolase 1 Antibody (1G7) [NBP2-59457] - Lane 1: PC3 cell lysates, Lane 2: MCF7 cell lysates, Lane 3: 293T cell lysates, Lane 4: HeLa cell lysates](http://images.novusbio.com/fullsize/Enolase-1-Antibody-1G7-Western-Blot-NBP2-59457-img0001.jpg)
![Western Blot: Enolase 1 Antibody (1G7) [NBP2-59457] - Analysis The recombinant protein (50ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ENO1, 2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: ENO1 (Alpha-enolase) recombinant proteinLane 2.: ENO2 (Gamma-enolase) recombinant proteinLane 3.: ENO3 (Beta-enolase) recombinant protein](http://images.novusbio.com/fullsize/Enolase-1-Antibody-1G7-Western-Blot-NBP2-59457-img0005.jpg)
![Western Blot: Enolase 1 Antibody (186) [NBP2-43701] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Enolase 1 Antibody (186) diluted at 1:10000. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Enolase-1-Antibody-186-Western-Blot-NBP2-43701-img0005.jpg "Western Blot: Enolase 1 Antibody (186) [NBP2-43701] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Enolase 1 Antibody (186) diluted at 1:10000. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.")
![Western Blot: Enolase 1 Antibody (186) [NBP2-43701] - Analysis of A. 30 ug A549 whole cell lysate/extract B. 30 ug H1299 whole cell lysate/extract C. 30 ug HCT116 whole cell lysate/extract 10 % SDS-PAGE ENO1 antibody [186] dilution: 1:10000.](http://images.novusbio.com/fullsize/Enolase-1-Antibody-186-Western-Blot-NBP2-43701-img0001.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Enolase 1 Antibody (186) [NBP2-43701] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ENO1 antibody [186].](http://images.novusbio.com/fullsize/Enolase-1-Antibody-186-Western-Blot-NBP2-43701-img0003.jpg)
![Western Blot: Enolase 1 Antibody (186) [NBP2-43701] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Enolase 1 Antibody (186) diluted at 1:10000. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Enolase-1-Antibody-186-Western-Blot-NBP2-43701-img0004.jpg)
![Western Blot: Enolase 1 Antibody (2217) [NBP2-43702] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ENO1 antibody diluted at 1:10000. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Enolase-1-Antibody-2217-Western-Blot-NBP2-43702-img0004.jpg "Western Blot: Enolase 1 Antibody (2217) [NBP2-43702] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ENO1 antibody diluted at 1:10000. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.")
![Western Blot: Enolase 1 Antibody (2217) [NBP2-43702] - Analysis of A. 30 ug A549 whole cell lysate/extract B. 30 ug H1299 whole cell lysate/extract C. 30 ug HCT116 whole cell lysate/extract 10 % SDS-PAGE ENO1 antibody [2217] dilution: 1:10000.](http://images.novusbio.com/fullsize/Enolase-1-Antibody-2217-Western-Blot-NBP2-43702-img0001.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Enolase 1 Antibody (2217) [NBP2-43702] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ENO1 antibody [2217].](http://images.novusbio.com/fullsize/Enolase-1-Antibody-2217-Western-Blot-NBP2-43702-img0002.jpg)
![Western Blot: Enolase 1 Antibody (2217) [NBP2-43702] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with ENO1 antibody diluted at 1:10000. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Enolase-1-Antibody-2217-Western-Blot-NBP2-43702-img0003.jpg)
![Western Blot: Enolase 1 Antibody (SR1812) [NBP3-21624] - Western blot analysis using NBP3-21624 in (1)MCF-7 whole cell lysate; (2)Rat brain tissue lysate.](http://images.novusbio.com/fullsize/nbp3-21624_rabbit-enolase-1-mab-sr1812-5720231624739.jpg "Western Blot: Enolase 1 Antibody (SR1812) [NBP3-21624] - Western blot analysis using NBP3-21624 in (1)MCF-7 whole cell lysate; (2)Rat brain tissue lysate.")
![Immunohistochemistry: Enolase 1 Antibody (SR1812) [NBP3-21624] - Immunohistochemical analysis of paraffin-embedded human colon, using NBP3-21624.](http://images.novusbio.com/fullsize/nbp3-21624_rabbit-enolase-1-mab-sr1812-57202317161830.jpg)
![Western Blot: Enolase 1 Antibody [NBP2-68874] - Analysis in human cell line RT-4, human cell line U-251 MG, human plasma, human liver tissue and human tonsil tissue.](http://images.novusbio.com/fullsize/Enolase-1-Antibody-Western-Blot-NBP2-68874-img0001.jpg "Western Blot: Enolase 1 Antibody [NBP2-68874] - Analysis in human cell line RT-4, human cell line U-251 MG, human plasma, human liver tissue and human tonsil tissue.")
![Immunocytochemistry/Immunofluorescence: Enolase 1 Antibody [NBP2-68874] - Staining of human cell line A-431 shows localization to plasma membrane & cytosol.](http://images.novusbio.com/fullsize/Enolase-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-68874-img0002.jpg)
![Western Blot: Enolase 1 Antibody (9E5R2) [NBP3-33187] - Western blot analysis of various lysates using Enolase 1 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 3s.](http://images.novusbio.com/fullsize/nbp3-33187_rabbit-enolase-1-mab-9e5r2-912202415951.jpg "Western Blot: Enolase 1 Antibody (9E5R2) [NBP3-33187] - Western blot analysis of various lysates using Enolase 1 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 3s.")
![Western Blot: Enolase 1 Antibody (A6) [NBP3-27947] - Antibody: Enolase 1 at 1/1000 dilutionSample: NIH-3T3 at 30ugSecondary: HRP conjugated anti-Rabbit Fc at 1/50000 dilutionPredicted band size: 47 kDaObserved band size: 47 kDaGel concentration: 4-20%](http://images.novusbio.com/fullsize/nbp3-27947_rabbit-enolase-1-mab-a6-2711202420171797.jpg "Western Blot: Enolase 1 Antibody (A6) [NBP3-27947] - Antibody: Enolase 1 at 1/1000 dilutionSample: NIH-3T3 at 30ugSecondary: HRP conjugated anti-Rabbit Fc at 1/50000 dilutionPredicted band size: 47 kDaObserved band size: 47 kDaGel concentration: 4-20%")