![Western Blot: Enolase 1 Antibody (1G7) [NBP2-59457] - The recombinant Enolase 1 protein [NBP1-30262] (50ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human [NBP2-59457] (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: ENO1 (Alpha-enolase) recombinant protein.](http://images.novusbio.com/fullsize/Enolase-1-Antibody-1G7-Western-Blot-NBP2-59457-img0006.jpg "Western Blot: Enolase 1 Antibody (1G7) [NBP2-59457] - The recombinant Enolase 1 protein [NBP1-30262] (50ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human [NBP2-59457] (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: ENO1 (Alpha-enolase) recombinant protein.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF |
| Clone | 1G7 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Recombinant human Enolase 1 (1-434aa) purified from E. coli |
| Isotype | IgG2a Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | ENO1 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.4), 10% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
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| Gene Symbol | ENO1 |
![Immunocytochemistry/Immunofluorescence: Enolase 1 Antibody (1G7) [NBP2-59457] - Analysis of ENO1, 2 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ENO1, 2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/Enolase-1-Antibody-1G7-Immunocytochemistry-Immunofluorescence-NBP2-59457-img0002.jpg "Immunocytochemistry/Immunofluorescence: Enolase 1 Antibody (1G7) [NBP2-59457] - Analysis of ENO1, 2 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ENO1, 2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).")
![Flow Cytometry: Enolase 1 Antibody (1G7) [NBP2-59457] - Analysis of ENO1, 2 in LNCap cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).](http://images.novusbio.com/fullsize/Enolase-1-Antibody-1G7-Flow-Cytometry-NBP2-59457-img0003.jpg "Flow Cytometry: Enolase 1 Antibody (1G7) [NBP2-59457] - Analysis of ENO1, 2 in LNCap cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).")
![Western Blot: Enolase 1 Antibody (1G7) [NBP2-59457] - Lane 1: PC3 cell lysates, Lane 2: MCF7 cell lysates, Lane 3: 293T cell lysates, Lane 4: HeLa cell lysates](http://images.novusbio.com/fullsize/Enolase-1-Antibody-1G7-Western-Blot-NBP2-59457-img0001.jpg "Western Blot: Enolase 1 Antibody (1G7) [NBP2-59457] - Lane 1: PC3 cell lysates, Lane 2: MCF7 cell lysates, Lane 3: 293T cell lysates, Lane 4: HeLa cell lysates")
![Western Blot: Enolase 1 Antibody (1G7) [NBP2-59457] - Analysis The recombinant protein (50ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ENO1, 2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: ENO1 (Alpha-enolase) recombinant proteinLane 2.: ENO2 (Gamma-enolase) recombinant proteinLane 3.: ENO3 (Beta-enolase) recombinant protein](http://images.novusbio.com/fullsize/Enolase-1-Antibody-1G7-Western-Blot-NBP2-59457-img0005.jpg "Western Blot: Enolase 1 Antibody (1G7) [NBP2-59457] - Analysis The recombinant protein (50ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ENO1, 2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: ENO1 (Alpha-enolase) recombinant proteinLane 2.: ENO2 (Gamma-enolase) recombinant proteinLane 3.: ENO3 (Beta-enolase) recombinant protein")
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