Novus Biologicals products are now on bio-techne.com

eIF4B Antibody (1F5) - BSA Free

Name: eIF4B Antibody (1F5) - BSA Free
Brand: Novus Biologicals
SKU: NBP2-37488
Price: 449 USD

Submit a Review

Images

(

Enlarge)

Western Blot: eIF4B Antibody (1F5) [NBP2-37488] - Western blot analysis using EIF4B mouse mAb against A549 (1), A431 (2), HepG2 (3), HEK293 (4), HeLa (5), Jurkat (6), K562 (7), NIH3T3 (8), and MCF-7 (9) cell lysate.

Immunocytochemistry/ Immunofluorescence: eIF4B Antibody (1F5) [NBP2-37488] - Immunofluorescence analysis of NIH3T3 cells using EIF4B mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been ...read more

Immunohistochemistry: eIF4B Antibody (1F5) [NBP2-37488] - Immunohistochemical analysis of paraffin-embedded colon cancer tissues using EIF4B mouse mAb with DAB staining.

Flow Cytometry: eIF4B Antibody (1F5) [NBP2-37488] - Flow cytometric analysis of HeLa cells using EIF4B mouse mAb (green) and negative control (red).

Western Blot: eIF4B Antibody (1F5) [NBP2-37488] - Western blot analysis using EIF4B mAb against human EIF4B (AA: 381-585) recombinant protein. (Expected MW is 48.3 kDa)

ELISA: eIF4B Antibody (1F5) [NBP2-37488] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Product Details

Summary

Reactivity	Hu, MuSpecies Glossary
Applications	WB, ELISA, Flow, ICC/IF, IHC, CyTOF-ready
Clone	1F5
Clonality	Monoclonal
Host	Mouse
Conjugate	Unconjugated
Format	BSA Free
Concentration	1 mg/ml

Order Details

View Available Formulations

Catalog# & Formulation	Size	Price
BSA Free NBP2-37488-0.025ml

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

eIF4B Antibody (1F5) - BSA Free Summary

Immunogen	Purified recombinant fragment of human eIF4B expressed in E. Coli.
Isotype	IgG2b
Clonality	Monoclonal
Host	Mouse
Gene	EIF4B
Purity	Protein G purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Applications/Dilutions

Dilutions	CyTOF-ready ELISA 1:10000 Flow Cytometry 1:200 - 1:400 Immunocytochemistry/ Immunofluorescence 1:200 - 1:1000 Immunohistochemistry 1:200 - 1:1000 Immunohistochemistry-Paraffin 1:200 - 1:1000 Western Blot 1:500 - 1:2000
Application Notes	This antibody is Cytof ready.
Theoretical MW	80 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer	PBS
Preservative	0.05% Sodium Azide
Concentration	1 mg/ml
Purity	Protein G purified

Alternate Names for eIF4B Antibody (1F5) - BSA Free

Eif4a2
eIF4B
EIF-4B
eukaryotic translation initiation factor 4B
PRO1843

Background

Eukaryotic initiation factor 4B (eIF4B) is a factor required for the binding of mRNA to ribosomes for the initiation of translation. eIF4B has been demonstrated to modulate the helicase activity of eIF4A, a component of the eIF4F complex

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

eIF4A1 Antibody

NBP2-24632

Immunohistochemistry-Paraffin eIF4A1 Antibody

(2 Publications)

Species: Ca, Ch, Hu, Mu, Pm, Rt

Applications: IHC, IHC-P, WB

eIF4A2 Antibody

NBP2-24529

Immunohistochemistry-Paraffin eIF4A2 Antibody

(1 Review)

(1 Publication)

Species: Ca, Ch, Hu, Mu, Pm

Applications: IHC, IHC-P, Simple Western, WB

eIF4G1 Antibody

NB100-268

(26 Publications)

Species: Hu, Po

Applications: IP, WB

eIF4E [p Ser209]
Antibody

NB100-81898

Immunocytochemistry/ Immunofluorescence eIF4E [p Ser209] Antibody

Immunohistochemistry-Paraffin eIF4E [p Ser209] Antibody

(1 Publication)

Species: Hu, Po

Applications: ICC/IF, IHC, IHC-P, WB

Ribosomal Protein
S6/RPS6 Antibody

NB100-1595

Western Blot Ribosomal Protein S6/RPS6 Antibody

Immunoprecipitation Ribosomal Protein S6/RPS6 Antibody

(1 Review)

(6 Publications)

Species: Hu, Mu

Applications: IHC, IHC-P, IP, WB

TOR/mTOR [p
Ser2448] Antibody

NB600-607

Western Blot TOR/mTOR [p Ser2448] Antibody

(2 Reviews)

(9 Publications)

Species: Hu, Rt

Applications: ELISA, IHC, IHC-P, WB

eIF2 alpha/EIF2S1
Antibody (OTI3H4)

NBP2-02669

Western Blot eIF2 alpha/EIF2S1 Antibody (OTI3H4)

Immunohistochemistry-Paraffin eIF2 alpha/EIF2S1 Antibody (OTI3H4)

Species: Ca, Hu, Pm, Mu, Pm, Rt

Applications: Flow, ICC/IF, IHC, IHC-P, WB

Tbp7 Antibody

NBP1-87799

(1 Publication)

Species: Hu, Mu, Rt

Applications: ICC/IF, IHC, IHC-P, WB

4EBP1 Antibody

NB200-157

(45 Publications)

Species: Hu

Applications: IHC, IHC-P, KO, WB

eIF4H Antibody

NBP1-83057

Immunohistochemistry-Paraffin eIF4H Antibody

Species: Hu, Mu, Rt

Applications: ICC/IF, IHC, IHC-P, WB

RSK1 [p Ser380]
Antibody (1024A) [Unc ...

MAB79671

Simple Western RSK1 [p Ser380] Antibody (1024A) [Unconjugated]

Western Blot RSK1 [p Ser380] Antibody (1024A) [Unconjugated]

(1 Publication)

Species: Hu, Mu, Rt

Applications: ICC, Simple Western, WB

Eukaryotic
translation initiatio ...

NBP2-00702

Species: Hu, Rt

Applications: Flow, ICC/IF, IHC, IHC-P, WB

PABP Antibody
(10E10) - BSA Free

NB120-6125

Western Blot PABP Antibody (10E10) - BSA Free

(1 Review)

(11 Publications)

Species: Bv, Ca, Dr(-), Hu, Mu(-), Pm, Xp

Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IP, MiAr, WB

AKT [p Ser473]
Antibody [Unconjugate ...

AF887

Simple Western AKT [p Ser473] Antibody [Unconjugated] - Pan Specific

Intracellular Staining by Flow Cytometry AKT [p Ser473] Antibody [Unconjugated] - Pan Specific

(49 Publications)

Species: Hu, Mu, Rt

Applications: CyTOF-ready, IHC, ICFlow, Simple Western, WB

EIF1A Antibody

NBP1-85808

Species: Hu, Mu, Rt

Applications: IHC, IHC-P, WB

eEF-2 Antibody
(4E2Z5)

NBP3-16866

Immunocytochemistry/ Immunofluorescence eEF-2 Antibody (4E2Z5)

Species: Hu, Mu, Rt

Applications: ICC/IF, IHC, IHC-P, WB

PIK3CA Antibody

NBP2-19804

Immunohistochemistry-Paraffin PIK3CA Antibody

(4 Publications)

Species: Hu, Mu

Applications: ICC/IF, IHC, IHC-P, WB

p21/CIP1/CDKN1A
Antibody (WA-1)

NBP2-29463

(24 Publications)

Species: Pm, Hu, Pm, Mu

Applications: Flow, ICC/IF, IHC, IHC-P, WB

ERK2 Antibody
[Unconjugated]

AF1230

Immunohistochemistry ERK2 Antibody [Unconjugated]

Knockout Validated ERK2 Antibody [Unconjugated]

(3 Publications)

Species: Hu, Mu, Rt

Applications: IHC, KO, WB

eIF4B Antibody
(1F5) - BSA Free

NBP2-37488

Species: Hu, Mu

Applications: WB, ELISA, Flow, ICC/IF, IHC, CyTOF-ready

Publications for eIF4B Antibody (NBP2-37488) (0)

There are no publications for eIF4B Antibody (NBP2-37488).

By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for eIF4B Antibody (NBP2-37488) (0)

There are no reviews for eIF4B Antibody (NBP2-37488). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.

Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

ICC/IF Video Protocol

FAQs for eIF4B Antibody (NBP2-37488) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

Isotype Controls

Additional eIF4B Products

Research Areas for eIF4B Antibody (NBP2-37488)

Find related products by research area.

Blogs on eIF4B

There are no specific blogs for eIF4B, but you can read our latest blog posts.

Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Western blot shows detection of Mouse Anti-Human/Mouse/Rat STAT3 Monoclonal Antibody (MAB1799) using a 1:1000 dilution of HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Goat anti-Mouse IgG Secondary Antibody [HRP]
HAF007

Contact Information

Product PDFs

SDS

PDF Datasheet

Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

Volume

Mass

Concentration

Review this Product

Be the first to review our eIF4B Antibody (1F5) - BSA Free and receive a gift card or discount.

Bioinformatics

Gene Symbol

EIF4B

Popular Products	Company Information	Support	Stay Connected
Antibodies Secondary Antibodies Isotype Controls Proteins and Peptides ELISA Kits Sample Size New Products	About Us Careers Novus Guarantee Contact Us Our Team	Technical Support FAQs Help Finding Antibodies Protocols

Visit our other sites

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

eIF4B Antibody (1F5) - BSA Free

Species Glossary

Images

Product Details

Order Details

eIF4B Antibody (1F5) - BSA Free Summary

Applications/Dilutions

Reactivity Notes

Packaging, Storage & Formulations

Alternate Names for eIF4B Antibody (1F5) - BSA Free

Background

Limitations

Customers Who Viewed This Item Also Viewed...

Publications for eIF4B Antibody (NBP2-37488) (0)

Reviews for eIF4B Antibody (NBP2-37488) (0)

Product General Protocols

Video Protocols

FAQs for eIF4B Antibody (NBP2-37488) (0)

Secondary Antibodies

Isotype Controls

Additional eIF4B Products

Research Areas for eIF4B Antibody (NBP2-37488)

Blogs on eIF4B

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Review this Product

Bioinformatics