eIF4B Antibodies
We offer eIF4B Antibodies for use in common research applications: ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot. Each eIF4B Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our eIF4B Antibodies can be used in a variety of model species: Bovine, Chicken, Human, Mouse, Primate, Rat. Use the list below to choose the eIF4B Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our eIF4B polyclonal antibodies and browse our eIF4B monoclonal antibody catalog.
Alternate Names for eIF4B Antibodies
anti-eIF4B antibody, anti-EIF-4B antibody, anti-eukaryotic translation initiation factor 4B antibody, anti-PRO1843 antibody
13 Results for "eif4b" in Primary Antibodies
![Western Blot: eIF4B Antibody [NB100-93308] - Detection of Human eIF4B on HeLa whole cell lysate using NB100-93308. eIF4B was also immunoprecipitated by rabbit anti-eIF4B antibody NB100-93307.](http://images.novusbio.com/fullsize/eIF4B-Antibody-Western-Blot-NB100-93308-img0002.jpg "<b>Independent Antibodies Validation. </b>Western Blot: eIF4B Antibody [NB100-93308] - Detection of Human eIF4B on HeLa whole cell lysate using NB100-93308. eIF4B was also immunoprecipitated by rabbit anti-eIF4B antibody NB100-93307.")
![Western Blot: eIF4B Antibody (1F5) [NBP2-37488] - Western blot analysis using EIF4B mouse mAb against A549 (1), A431 (2), HepG2 (3), HEK293 (4), HeLa (5), Jurkat (6), K562 (7), NIH3T3 (8), and MCF-7 (9) cell lysate.](http://images.novusbio.com/fullsize/eIF4B-Antibody-1F5-Western-Blot-NBP2-37488-img0003.jpg "Western Blot: eIF4B Antibody (1F5) [NBP2-37488] - Western blot analysis using EIF4B mouse mAb against A549 (1), A431 (2), HepG2 (3), HEK293 (4), HeLa (5), Jurkat (6), K562 (7), NIH3T3 (8), and MCF-7 (9) cell lysate.")
![Immunocytochemistry/Immunofluorescence: eIF4B Antibody (1F5) [NBP2-37488] - Immunofluorescence analysis of NIH3T3 cells using EIF4B mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/eIF4B-Antibody-1F5-Immunocytochemistry-Immunofluorescence-NBP2-37488-img0004.jpg)
![Immunohistochemistry: eIF4B Antibody (1F5) [NBP2-37488] - Immunohistochemical analysis of paraffin-embedded colon cancer tissues using EIF4B mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/eIF4B-Antibody-1F5-Immunohistochemistry-NBP2-37488-img0006.jpg)
![Flow Cytometry: eIF4B Antibody (1F5) [NBP2-37488] - Flow cytometric analysis of HeLa cells using EIF4B mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/eIF4B-Antibody-1F5-Flow-Cytometry-NBP2-37488-img0005.jpg)
![Western Blot: eIF4B Antibody (1F5) [NBP2-37488] - Western blot analysis using EIF4B mAb against human EIF4B (AA: 381-585) recombinant protein. (Expected MW is 48.3 kDa)](http://images.novusbio.com/fullsize/eIF4B-Antibody-1F5-Western-Blot-NBP2-37488-img0002.jpg)
![ELISA: eIF4B Antibody (1F5) [NBP2-37488] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/eIF4B-Antibody-1F5-ELISA-NBP2-37488-img0001.jpg)
![Western Blot: eIF4B Antibody [NBP2-16306] - A. 30 ug 293T whole cell extract B. 30 ug whole cell extract of human EIF4B-transfected 293T cells 7.5 % SDS-PAGE EIF4B antibody dilution: 1:5000](http://images.novusbio.com/fullsize/eIF4B-Antibody-Western-Blot-NBP2-16306-img0005.jpg "Western Blot: eIF4B Antibody [NBP2-16306] - A. 30 ug 293T whole cell extract B. 30 ug whole cell extract of human EIF4B-transfected 293T cells 7.5 % SDS-PAGE EIF4B antibody dilution: 1:5000")
![Immunocytochemistry/Immunofluorescence: eIF4B Antibody [NBP2-16306] - Analysis of paraformaldehyde-fixed MCF-7, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/eIF4B-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16306-img0002.jpg)
![Immunohistochemistry-Paraffin: eIF4B Antibody [NBP2-16306] - Paraffin-embedded mouse kidney. EIF4B antibody [N1N2], N-term dilution: 1:500.](http://images.novusbio.com/fullsize/eIF4B-Antibody-Immunohistochemistry-Paraffin-NBP2-16306-img0006.jpg)
![Western Blot: eIF4B Antibody [NBP2-16306] - Sample (30 ug of whole cell lysate) A: NT2D1 B: IMR32 C: U87-MG D: MCF-7 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/eIF4B-Antibody-Western-Blot-NBP2-16306-img0001.jpg)
![Western Blot: eIF4B Antibody [NBP2-16306] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EIF4B antibody diluted by 1:1000.](http://images.novusbio.com/fullsize/eIF4B-Antibody-Western-Blot-NBP2-16306-img0003.jpg)
![Western Blot: eIF4B Antibody [NBP2-16306] - Various whole cell extracts (30 ug) were separated by 7.5 % SDS-PAGE, and blotted with EIF4B antibody diluted by 1:1000](http://images.novusbio.com/fullsize/eIF4B-Antibody-Western-Blot-NBP2-16306-img0004.jpg)
![Western Blot: eIF4B Antibody [NBP2-24475] - analysis of eIF4B in Hela cell lysate in the 1) absence, 2) presence of immunizing peptide and 3) RAW using this antibody. 25 ug/ml.](http://images.novusbio.com/fullsize/eIF4B-Antibody-Western-Blot-NBP2-24475-img0001.jpg "Western Blot: eIF4B Antibody [NBP2-24475] - analysis of eIF4B in Hela cell lysate in the 1) absence, 2) presence of immunizing peptide and 3) RAW using this antibody. 25 ug/ml.")
![Western Blot: eIF4B [p Ser406] Antibody (JE63-80) [NBP3-32763] - Western blot analysis of eIF4B on different lysates with Rabbit anti-eIF4B antibody (NBP3-32763) at 1/50,000 dilution. Lane 1: 293T cell lysate Lane 2: 293T treated with 100nM Calyculin A for 15 minutes cell lysate Lane 3: HeLa cell lysate Lane 4: HeLa treated with 100ng/mL Calyculin A for 30 minutes cell lysate Lane 5: Jurkat cell lysate Lane 6: Jurkat treated with 100nM Calyculin A for 30 minutes cell lysate Lane 7: NIH/3T3 cell lysate Lane 8: NIH/3T3 treated with 100nM Calyculin A for 30 minutes cell lysate Lane 9: PC-12 cell lysate Lane 10: PC-12 treated with 100nM Calyculin A for 30 minutes cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 69 kDa Observed band size: 80 kDa Exposure time: 21 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32763) at 1/50,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32763_rabbit-eif4b-p-ser406-mab-je63-80-288202412461610.jpg "Western Blot: eIF4B [p Ser406] Antibody (JE63-80) [NBP3-32763] - Western blot analysis of eIF4B on different lysates with Rabbit anti-eIF4B antibody (NBP3-32763) at 1/50,000 dilution. Lane 1: 293T cell lysate Lane 2: 293T treated with 100nM Calyculin A for 15 minutes cell lysate Lane 3: HeLa cell lysate Lane 4: HeLa treated with 100ng/mL Calyculin A for 30 minutes cell lysate Lane 5: Jurkat cell lysate Lane 6: Jurkat treated with 100nM Calyculin A for 30 minutes cell lysate Lane 7: NIH/3T3 cell lysate Lane 8: NIH/3T3 treated with 100nM Calyculin A for 30 minutes cell lysate Lane 9: PC-12 cell lysate Lane 10: PC-12 treated with 100nM Calyculin A for 30 minutes cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 69 kDa Observed band size: 80 kDa Exposure time: 21 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32763) at 1/50,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: eIF4B [p Ser406] Antibody (JE63-80) [NBP3-32763] - Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-eIF4B antibody (NBP3-32763) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32763) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32763_rabbit-eif4b-p-ser406-mab-je63-80-29820241411549.jpg)
![Immunocytochemistry/ Immunofluorescence: eIF4B [p Ser406] Antibody (JE63-80) [NBP3-32763] - Immunocytochemistry analysis of Jurkat cells treated with or without lambda pp labeling eIF4B with Rabbit anti-eIF4B antibody (NBP3-32763) at 1/20,000 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-eIF4B antibody (NBP3-32763) at 1/20,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32763_rabbit-eif4b-p-ser406-mab-je63-80-29202410484885.jpg)
![Flow Cytometry: eIF4B [p Ser406] Antibody (JE63-80) [NBP3-32763] - Flow cytometric analysis of Jurkat cells labeling eIF4B. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32763, 1/5,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32763_rabbit-eif4b-p-ser406-mab-je63-80-29202416453295.jpg)
![Western Blot: eIF4B Antibody [NBP2-24504] - analysis of eIF4B in K562 cell lysate in the 1) absence and2) presence of immunizing peptide, and 3) RAW cell lysate using this antibody. 005 ug/ml, 0.005 ug/ml and 1.0 ug/ml respectively.](http://images.novusbio.com/fullsize/eIF4B-Antibody-Western-Blot-NBP2-24504-img0001.jpg "Western Blot: eIF4B Antibody [NBP2-24504] - analysis of eIF4B in K562 cell lysate in the 1) absence and2) presence of immunizing peptide, and 3) RAW cell lysate using this antibody. 005 ug/ml, 0.005 ug/ml and 1.0 ug/ml respectively.")
![Western Blot: eIF4B Antibody [NBP2-33284] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/eIF4B-Antibody-Western-Blot-NBP2-33284-img0005.jpg "Western Blot: eIF4B Antibody [NBP2-33284] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp")
![Immunocytochemistry/Immunofluorescence: eIF4B Antibody [NBP2-33284] - Immunofluorescent staining of human cell line MCF7 shows localization to cytosol.](http://images.novusbio.com/fullsize/eIF4B-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-33284-img0008.jpg)
![Immunohistochemistry-Paraffin: eIF4B Antibody [NBP2-33284] - Staining of human rectum shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/eIF4B-Antibody-Immunohistochemistry-Paraffin-NBP2-33284-img0007.jpg)
![Western Blot: eIF4B [p Ser 406] Antibody (SR2202) [NBP3-21605] - Western blot analysis using NBP3-21605 in (1) HeLa cell lysate; (2) HeLa cell treated with Alkaline Phosphatase lysate.](http://images.novusbio.com/fullsize/nbp3-21605_rabbit-eif4b-p-ser-406-mab-sr2202-57202317215166.jpg "Western Blot: eIF4B [p Ser 406] Antibody (SR2202) [NBP3-21605] - Western blot analysis using NBP3-21605 in (1) HeLa cell lysate; (2) HeLa cell treated with Alkaline Phosphatase lysate.")
![Western Blot: eIF4B Antibody (S07-2H2) [NBP3-19859] - Western blot detection of eIF4B in Hela, A549, HL-60, U2OS cell lysates using NBP3-19859 (1:1000 diluted). Predicted band size: 69kDa. Observed band size: 80kDa.](http://images.novusbio.com/fullsize/eIF4B-Antibody-S07-2H2-Western-Blot-NBP3-19859-img0002.jpg "Western Blot: eIF4B Antibody (S07-2H2) [NBP3-19859] - Western blot detection of eIF4B in Hela, A549, HL-60, U2OS cell lysates using NBP3-19859 (1:1000 diluted). Predicted band size: 69kDa. Observed band size: 80kDa.")
![Immunohistochemistry-Paraffin: eIF4B Antibody (S07-2H2) [NBP3-19859] - Immunohistochemistry of eIF4B in paraffin-embedded Human lung cancer tissue using NBP3-19859 at dilution 1/50](http://images.novusbio.com/fullsize/eIF4B-Antibody-S07-2H2-Immunohistochemistry-Paraffin-NBP3-19859-img0001.jpg)
![Western Blot: eIF4B [p Ser406] Antibody (JE58-32) [NBP3-32762] - Western blot analysis of eIF4B on different lysates with Rabbit anti-eIF4B antibody (NBP3-32762) at 1/2,000 dilution. Lane 1: NIH/3T3 cell lysate Lane 2: HeLa cell lysate Lane 3: NIH/3T3 treated pp for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 69 kDa Observed band size: 80 kDa Exposure time: 45 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32762) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32762_rabbit-eif4b-p-ser406-mab-je58-32-288202412442972.jpg "Western Blot: eIF4B [p Ser406] Antibody (JE58-32) [NBP3-32762] - Western blot analysis of eIF4B on different lysates with Rabbit anti-eIF4B antibody (NBP3-32762) at 1/2,000 dilution. Lane 1: NIH/3T3 cell lysate Lane 2: HeLa cell lysate Lane 3: NIH/3T3 treated pp for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 69 kDa Observed band size: 80 kDa Exposure time: 45 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32762) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")