PABP Antibody (10E10) - BSA Free

The poly(A)-binding protein (PABP), which is found complexed to the 3-prime poly(A) tail of eukaryotic mRNA, is required for poly(A) shortening and translation initiation. Grange et al. (1987) isolated a melanoma cell cDNA encoding human PABP. The predicted 633-amino acid protein contains 4 repeats of an approximately 80-amino acid unit in its N-terminal half. The authors found that this repeat region is highly conserved between human and yeast PABP and is sufficient for poly(A) binding. In vitro translation of the human PABP cDNA yielded a protein with an apparent molecular mass of 73 kD by SDS-PAGE. Northern blot analysis indicated that PABP is expressed as a 2.9-kb mRNA in human melanoma cells. Gorlach et al. (1994) noted that each of the 4 repeats of PABP is a ribonucleoprotein (RNP) consensus sequence RNA-binding domain. They determined that PABP has a pI of approximately 10.3 and is a very abundant, stable protein. Immunofluorescence studies of mammalian cells indicated that PABP is located exclusively in the cytoplasm. However, using both indirect immunofluorescence and tagging of PABP1 by fusion to the green fluorescent protein (GFP), Afonina et al. (1998) demonstrated that PABP1 shuttles between the nucleus and cytoplasm. PABP1 accumulated in the nucleus when transcription was inhibited, suggesting that active transcription is required for nuclear export of PABP1.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: IP, Immunoprecipitation, WB, Western Blot.

Showing Publications 1 - 10 of 11. Show All 11 Publications. Collapse Publications.

Publications using NB120-6125	Applications	Species
Nagel M, No beta M, Xu J et al. The kinesin motor KIF1C is a putative transporter of the exon junction complex in neuronal cells bioRxiv 2022-08-26 [PMID: 36316088] (WB, Human)	WB	Human
Shen TJ, Chen CL, Tsai TT et al. Hyperglycemia exacerbates dengue virus infection by facilitating poly(A)-binding protein-mediated viral translation JCI Insight 2022-11-08 [PMID: 36125898] (Immunoprecipitation, Western Blot)	Immunoprecipitation, Western Blot
Kim B, Park Y, Hwang HJ et al. Single polysome analysis of mRNP Biochemical and biophysical research communications 2022-06-08 [PMID: 35716598]
Genois MM, Gagné JP, Yasuhara T, et al. CARM1 regulates replication fork speed and stress response by stimulating PARP1 Molecular cell 2020-12-30 [PMID: 33412112]
Chazal PE, Daguenet E, Wendling C et al. EJC core component MLN51 interacts with eIF3 and activates translation Proc Natl Acad Sci U S A 2013-03-25 [PMID: 23530232] (WB, Human)	WB	Human
Sagliocco F, Laloo B, Cosson B et al. The ARE-associated factor AUF1 binds poly(A) in vitro in competition with PABP. Biochem J. 2006-12-01 [PMID: 16834569] (WB, Mouse)	WB	Mouse
Larralde O, Smith RW, Wilkie GS et al. Direct stimulation of translation by the multifunctional herpesvirus ICP27 protein. J Virol. 2006-02-01 [PMID: 16415034] (WB, Human)	WB	Human
Nelson PT, Hatzigeorgiou AG, Mourelatos Z. miRNP:mRNA association in polyribosomes in a human neuronal cell line. RNA. 2004-03-01 [PMID: 14970384] (WB, Human)	WB	Human
Sladic RT, Lagnado CA, Bagley CJ, Goodall GJ. Human PABP binds AU-rich RNA via RNA-binding domains 3 and 4. Eur J Biochem. 2004-01-01 [PMID: 14717712] (IP, Human)	IP	Human
Lee J, Bedford MT. PABP1 identified as an arginine methyltransferase substrate using high-density protein arrays. EMBO Rep. 2002-03-01 [PMID: 11850402] (IP, Human)	IP	Human
Grosset C, Chen CY, Xu N et al. A mechanism for translationally coupled mRNA turnover: interaction between the poly(A) tail and a c-fos RNA coding determinant via a protein complex. Cell. 2000-09-29 [PMID: 11051545] (WB, Mouse)	WB	Mouse
Show All 11 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
Enlarge	5 reviewed by: Stephanie DeWitte-Orr	WB	Human, Oncorhynchus mykiss, Rainbow trout	05/02/2017	View Summary Application Western Blot Sample Tested HFF-1 cells total lysate,RTG-2 cells total lysate Species Human, Oncorhynchus mykiss, Rainbow trout Lot A-1

Array NB120-6125

• PABP Antibodies
• PABP Lysate
• PABP Proteins