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PAIP2 Antibody

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Immunocytochemistry/Immunofluorescence: PAIP2 Antibody [NBP2-38802] - Immunofluorescent staining of human cell line HeLa shows localization to vesicles.
Immunocytochemistry/ Immunofluorescence: PAIP2 Antibody [NBP2-38802] - Immunofluorescent staining of human cell line HeLa shows localization to vesicles.
Immunohistochemistry: PAIP2 Antibody [NBP2-38802] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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PAIP2 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: KDPSRSSTSPSIINEDVIINGHSHEDDNPFAEYM
Predicted Species
Mouse (94%), Rat (94%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PAIP2
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
PAIP2 Protein (NBP2-38802PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for PAIP2 Antibody

  • MGC72018
  • PABC1-interacting protein 2
  • PABP-interacting protein 2
  • PAIP-2
  • PAIP2APoly(A)-binding protein-interacting protein 2
  • poly(A) binding protein interacting protein 2
  • polyA-binding protein-interacting protein 2
  • polyadenylate-binding protein-interacting protein 2

Background

PAIP2, also known as Poly(A)-binding protein-interacting protein 2, acts as a repressor in the regulation of translation initiation of poly(A)-containing mRNAs. Its inhibitory activity on translation is mediated via its action on PABPC1. It displaces the interaction of PABPC1 with poly(A) RNA and competes with PAIP1 for binding to PABPC1. Its association with PABPC1 results in disruption of the cytoplasmic poly(A) RNP structure organization. Recombinant PAIP2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Bioinformatics

Gene Symbol PAIP2
Uniprot