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Dynamin 2 Antibody

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Western Blot: Dynamin 2 Antibody [NBP2-16244] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Dynamin 2 antibody diluted at 1:5000. HRP-conjugated anti-rabbit IgG antibody ...read more
Immunocytochemistry/ Immunofluorescence: Dynamin 2 Antibody [NBP2-16244] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: Dynamin 2 Antibody [NBP2-16244] - Human breast carcinoma.
Western Blot: Dynamin 2 Antibody [NBP2-16244] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Dynamin 2 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG ...read more
Western Blot: Dynamin 2 Antibody [NBP2-16244] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Dynamin 2 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG ...read more
Western Blot: Dynamin 2 Antibody [NBP2-16244] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Dynamin 2 antibody [N1N3] (NBP2-16244) diluted at 1:5000. The ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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Dynamin 2 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Dynamin 2. The exact sequence is proprietary.
Localization
Cytoplasm; cytoskeleton; Cell junction; synapse; postsynaptic cell membrane; postsynaptic density
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DNM2
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:10000
Theoretical MW
98 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Antigen Affinity-purified

Alternate Names for Dynamin 2 Antibody

  • CMTDI1CMT2M
  • CMTDIBdynamin II
  • DI-CMTB
  • DNM2
  • DYN2
  • DYN2EC 3.6.5.5
  • Dynamin 2
  • dynamin-2
  • DYNII

Background

Dynamins represent one of the subfamilies of GTP-binding proteins. These proteins share considerable sequence similarity over the N-terminal portion of the molecule, which contains the GTPase domain. Dynamins are associated with microtubules. They have been implicated in cell processes such as endocytosis and cell motility, and in alterations of the membrane that accompany certain activities such as bone resorption by osteoclasts. Dynamins bind many proteins that bind actin and other cytoskeletal proteins. Dynamins can also self-assemble, a process that stimulates GTPase activity. Four alternatively spliced transcripts encoding different proteins have been described. Additional alternatively spliced transcripts may exist, but their full-length nature has not been determined. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Research Areas for Dynamin 2 Antibody (NBP2-16244)

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Blogs on Dynamin 2.

EEA1 Antibodies in Endosomal Transport and Signalling Studies
EEA1, or Early Endosome Antigen 1, is a phospholipid-binding protein essential for endosomal membrane trafficking and fusion, and is a useful endosomal marker. We at Novus Biologicals have an extensive EEA1 antibody catalog, with antibodies that have ...  Read full blog post.

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Bioinformatics

Gene Symbol DNM2
Uniprot