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Dicer Antibody

Images

 
Immunohistochemistry-Paraffin: Dicer Antibody [NBP2-23891] - Analysis of human testis using Dicer antibody at 1:200.
Flow Cytometry: Dicer Antibody [NBP2-23891] - Analysis of HeLa cells transiently transfected with Dicer or control siRNA and analyzed for Dicer expression using this antibody. Purple represents isotype control staining.
ELISA: Dicer Antibody [NBP2-23891] - ELISA assay of HeLa cell lysate transiently transfected with Dicer or control siRNA and analyzed for Dicer expression using Dicer antibody followed by an anti rabbit IgG-HRP antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA, Flow, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Dicer Antibody Summary

Immunogen
Within the range of amino acids 1423-1463 of human Dicer protein were used as the immunogen for this antibody.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DICER1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:200
  • Flow (Intracellular) reported in scientific literature (PMID 16823371)
  • Flow Cytometry 1:100 - 1:1000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:200
Publications
Read Publication using NBP2-23891.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Crude whole Rabbit sera, 50% Glycerol
Preservative
0.025% Sodium Azide
Purity
Unpurified

Alternate Names for Dicer Antibody

  • DCR1
  • dicer 1, double-stranded RNA-specific endoribonuclease
  • dicer 1, ribonuclease type III
  • Dicer
  • Dicer1, Dcr-1 homolog (Drosophila)
  • EC 3.1.26
  • EC 3.1.26.-
  • EC 3.1.26.3
  • Helicase MOI
  • Helicase with RNase motif
  • helicase-moi
  • HERNADicer1, Dcr-1 homolog
  • K12H4.8-LIKE
  • KIAA0928endoribonuclease Dicer

Background

Dicer encodes a protein possessing an RNA helicase motif containing a DEXH box in its amino terminus and an RNA motif in the carboxy terminus. The encoded protein functions as a ribonuclease and is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small RNA component that represses gene expression. Two transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Dicer Antibody (NBP2-23891)(1)

We have publications tested in 1 application: Flow Cytometry Control.


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Product General Protocols

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Secondary Antibodies

 

Isotype Controls

Additional Dicer Products

Research Areas for Dicer Antibody (NBP2-23891)

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Blogs on Dicer.

Slicing and Dicing RNA with Dicer
Dicer is an RNaseIII-like enzyme capable of cleaving double-stranded RNA (dsRNA) into smaller 21-23 nt RNA fragments known as short interfering RNA (siRNAs). It targets the selective degradation of complementary RNAs in a posttranscriptional gene sile...  Read full blog post.

Ago2 antibodies and dicer-independent biogenesis of miRNA
Ago2, also called eIF2C2, antibody is one of 37 reagents targeted to the Argonaute protein family that we at Novus Biologicals have in our antibody catalogue. Argonaute proteins are encoded by genes which play an important role in regulating the contr...  Read full blog post.

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Bioinformatics

Gene Symbol DICER1
Uniprot