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SLC1A5 Antibody

Images

 
Orthogonal Strategies: Immunohistochemistry-Paraffin: SLC1A5 Antibody [NBP1-89327] - Staining in human prostate and skeletal muscle tissues . Corresponding SLC1A5 RNA-seq data are presented for the same tissues.
Western Blot: SLC1A5 Antibody [NBP1-89327] - Analysis in human cell line NB4.
Immunocytochemistry/ Immunofluorescence: SLC1A5 Antibody [NBP1-89327] - Staining of human cell line A-431 shows localization to plasma membrane. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: SLC1A5 Antibody [NBP1-89327] - Staining in human hepatocellular carcinoma. Image from a verified customer review.
Western Blot: SLC1A5 Antibody [NBP1-89327] - Analysis in control (vector only transfected HEK293T lysate) and SLC1A5 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T ...read more
Immunohistochemistry-Paraffin: SLC1A5 Antibody [NBP1-89327] - Staining of human placenta shows strong membranous positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: SLC1A5 Antibody [NBP1-89327] - Staining of human prostate shows strong membranous positivity in glandular cells.
Immunohistochemistry-Paraffin: SLC1A5 Antibody [NBP1-89327] - Staining of human rectum shows moderate to strong membranous positivity in glandular cells.
Immunohistochemistry-Paraffin: SLC1A5 Antibody [NBP1-89327] - Staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.

Product Details

Summary
Reactivity Hu, Mu, PoSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

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SLC1A5 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: VDRTESRSTEPELIQVKSELPLDPLPVPTEEGNPLLKHYRGPAGDATVASEKESVM
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SLC1A5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
SLC1A5 Recombinant Protein Antigen (NBP1-89327PEP)
Reviewed Applications
Read 1 Review rated 5
using
NBP1-89327 in the following applications:

Publications
Read Publications using
NBP1-89327 in the following applications:

Reactivity Notes

Use in Porcine reported in scientific literature (PMID:34488623). Mouse reactivity reported in scientific literature (PMID: 31387898).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for SLC1A5 Antibody

  • AAAT
  • Alanine/serine/cysteine transporter 2
  • ASC amino acid transporter-2
  • ASCT2
  • ATB(0)
  • ATBO
  • baboon M7 virus receptor
  • M7V1
  • M7VS1
  • neutral amino acid transporter B
  • neutral amino acid transporter B(0)
  • R16
  • RD114 virus receptor
  • RD114/simian type D retrovirus receptor
  • RDR
  • RDRC
  • SLC1A5
  • Sodium-dependent neutral amino acid transporter type 2
  • solute carrier family 1 (neutral amino acid transporter), member 5
  • Solute carrier family 1 member 5

Background

SLC1A5 has a broad substrate specificity, a preference for zwitterionic amino acids, and a sodium-dependence. It accepts as substrates all neutral amino acids, including glutamine, asparagine, and branched-chain and aromatic amino acids, and excludes methylated

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SLC1A5 Antibody (NBP1-89327)(5)

We have publications tested in 3 confirmed species: Human, Mouse, Porcine.

We have publications tested in 5 applications: ICC/IF, IF/IHC, IHC-P, PLA, WB.


Filter By Application
ICC/IF
(2)
IF/IHC
(1)
IHC-P
(1)
PLA
(1)
WB
(2)
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Human
(1)
Mouse
(1)
Porcine
(1)
All Species

Review for SLC1A5 Antibody (NBP1-89327) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-89327:
Filter by Applications
IHC
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry SLC1A5 NBP1-89327
Enlarge
5
reviewed by:
Verified Customer
IHC Human 07/11/2017
View

Summary

ApplicationImmunohistochemistry
Sample TestedHepatocellular Carcinoma
SpeciesHuman

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SLC1A5 Antibody (NBP1-89327) (0)

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Recent Reviews

5
5
1
4
0
3
0
2
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1
0

Verified Customer
07/11/2017
Application: IHC
Species: Human

Bioinformatics

Gene Symbol SLC1A5
Entrez
Uniprot