![Western Blot: DIAPH-2 Antibody [NB600-280] - Samples: Whole cell lysate from 293T (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HeLa (H; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Diaphanous 2 antibody.](http://images.novusbio.com/fullsize/DIAPH-2-Antibody-Western-Blot-NB600-280-img0004.jpg "Western Blot: DIAPH-2 Antibody [NB600-280] - Samples: Whole cell lysate from 293T (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HeLa (H; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Diaphanous 2 antibody.")
| Reactivity | HuSpecies Glossary |
| Applications | WB |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1.0 mg/ml |
| Immunogen | The immunogen recognized by this antibody maps to a region between residues 50 and 100 of human Diaphanous Homolog 2 using the numbering given in entry NP_006720.1 (GeneID 1730). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | DIAPH2 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This antibody can be used for Western blot. *Suggested Working Dilutions: Western blot: 1:1,000-1:10,000 Immunoprecipitation: not tested Immunohistochemistry: not tested *Optimal working dilutions should be determined experimentally by the investigator. |
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| Theoretical MW | 125.6 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
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| Publication using NB600-280 | Applications | Species |
|---|---|---|
| Hu X, Guo J, Zheng L et al. The Heterochronic microRNA let-7 Inhibits Cell Motility by Regulating the Genes in the Actin Cytoskeleton Pathway in Breast Cancer. Mol Cancer Res 2013-03-01 [PMID: 23339187] (WB, Human) | WB | Human |
Secondary Antibodies |
Isotype Controls |
Research Areas for DIAPH-2 Antibody (NB600-280)Find related products by research area.
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![Western Blot: DIAPH-2 Antibody [NB600-280] - Whole cell lysate (50 ug) from HEK 293T cells. Antibody used at the indicated concentrations.](http://images.novusbio.com/fullsize/DIAPH-2-Antibody-Western-Blot-NB600-280-img0002.jpg "Western Blot: DIAPH-2 Antibody [NB600-280] - Whole cell lysate (50 ug) from HEK 293T cells. Antibody used at the indicated concentrations.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
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