![Western Blot: DIAPH3 Antibody (4D5) [H00081624-M01] - Analysis of DIAPH3 expression in transfected 293T cell line by DIAPH3 monoclonal antibody (M01), clone 4D5.Lane 1: DIAPH3 transfected lysate (Predicted MW: 98.1 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/DIAPH3-Antibody-4D5-Western-Blot-H00081624-M01-img0004.jpg "Western Blot: DIAPH3 Antibody (4D5) [H00081624-M01] - Analysis of DIAPH3 expression in transfected 293T cell line by DIAPH3 monoclonal antibody (M01), clone 4D5.Lane 1: DIAPH3 transfected lysate (Predicted MW: 98.1 KDa).Lane 2: Non-transfected lysate.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, ICC/IF |
| Clone | 4D5 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | DIAPH3 (NP_112194, 632 a.a. ~ 729 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PDILNFVDDLEPLDKASKVSVETLEKNLRQMGRQLQQLEKELETFPPPEDLHDKFVTKMSRFVISAKEQYETLSKLHENMEKLYQSIIGYYAIDVKKV |
| Specificity | DIAPH3 - diaphanous homolog 3 (Drosophila) (4D5) |
| Isotype | IgG2a Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | DIAPH3 |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Antibody Reactive Against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | IgG purified |
Secondary Antibodies |
Isotype Controls |
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![Immunocytochemistry/Immunofluorescence: DIAPH3 Antibody (4D5) [H00081624-M01] - Analysis of monoclonal antibody to DIAPH3 on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/DIAPH3-Antibody-4D5-Immunocytochemistry-Immunofluorescence-H00081624-M01-img0002.jpg "Immunocytochemistry/Immunofluorescence: DIAPH3 Antibody (4D5) [H00081624-M01] - Analysis of monoclonal antibody to DIAPH3 on HeLa cell . Antibody concentration 10 ug/ml.")
![Sandwich ELISA: DIAPH3 Antibody (4D5) [H00081624-M01] - Detection limit for recombinant GST tagged DIAPH3 is 1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/DIAPH3-Antibody-4D5-Sandwich-ELISA-H00081624-M01-img0003.jpg "Sandwich ELISA: DIAPH3 Antibody (4D5) [H00081624-M01] - Detection limit for recombinant GST tagged DIAPH3 is 1 ng/ml as a capture antibody.")
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