DIAPH1 Antibody - BSA Free

Images

 
Biological Strategies: Western Blot: DIAPH1 Antibody [NB600-231] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Diaphanous ...read more
Immunohistochemistry: DIAPH1 Antibody [NB600-231] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Diaphanous used at a dilution of 1:1,000 ( 1ug/ml). Detection: DAB
Genetic Strategies: Western Blot: DIAPH1 Antibody [NB600-231] - Whole cell lysate (50 ug/lane) from untreated or siRNA treated 293T cells. Antibody used at 0.1 ug/ml.
Immunohistochemistry-Paraffin: DIAPH1 Antibody [NB600-231] - FFPE section of human ovarian carcinoma. Affinity purified rabbit anti-Diaphanous1 used at a dilution of 1:100.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

DIAPH1 Antibody - BSA Free Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 1200 and the C-terminus (residue 1248) of human Diaphanous Homolog 1 using the numbering given in entry NP_005210.1 (GeneID 1729).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DIAPH1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:1000-1:5000
  • Immunohistochemistry-Paraffin 1:1000-1:5000
  • Knockdown Validated
  • Western Blot 1:5000-1:15000
Application Notes
By Western Blot this antibody recognizes a band at ~205 kDa, representing Diaphanous 1. Epitope Retrieval is not recommended.
Publications
Read Publications using NB600-231.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DIAPH1 Antibody - BSA Free

  • DIA1
  • diaphanous homolog 1 (Drosophila)
  • hDIA1
  • homolog) 1
  • LFHL1FLJ25265

Background

Diaphanous 1 is a member of the Formin homology (FH) family of cytoskeletal organizer proteins. It is required for cytokinesis and transcriptional activation of the serum response factor. It has also been suggested to contribute to localized actin polymerization and stress fiber formation, both by recruiting profiling to sites of Rho action and by cooperating with ROCK. The human Diaphanous 1 gene is associated with Premature Ovarian Failure (POF) and Nonsyndromic Deafness (DFNA1), an autosomal dominant, fully penetrant progressive hearing loss.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-58359
Species: Hu
Applications: IHC, IHC-P, WB
NBP2-25160
Species: Bv, Ca, Eq, Hu, Mu, Po, Rt
Applications: ICC/IF, IHC, WB
NBP1-85217
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-41304
Species: Hu, Po, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
H00081624-M01
Species: Hu
Applications: ELISA, ICC/IF, S-ELISA, WB
NB100-91273
Species: Hu, Mu, Pm, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP3-13785
Species: Hu
Applications: ELISA, Flow, ICC/IF, IHC-P, IP, PA, WB
H00342184-M07
Species: Hu
Applications: ELISA, ICC/IF, WB
NBP2-70411
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NBP1-31302
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, KO, WB
AF2635
Species: Hu
Applications: WB
H00010097-M01
Species: Hu
Applications: ELISA, IHC, IHC-P, S-ELISA, WB
NBP1-88461
Species: Hu
Applications: IHC, IHC-P
NB300-996
Species: Hu
Applications: Flow, ICC/IF, IHC, IHC-P, PEP-ELISA, WB
AF1444
Species: Mu
Applications: IHC, WB
NBP1-30955
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-90286
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
AF2685
Species: Hu
Applications: IHC, Simple Western, WB

Publications for DIAPH1 Antibody (NB600-231)(2)

Reviews for DIAPH1 Antibody (NB600-231) (0)

There are no reviews for DIAPH1 Antibody (NB600-231). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for DIAPH1 Antibody (NB600-231) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional DIAPH1 Products

Research Areas for DIAPH1 Antibody (NB600-231)

Find related products by research area.

Blogs on DIAPH1

There are no specific blogs for DIAPH1, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our DIAPH1 Antibody - BSA Free and receive a gift card or discount.

Bioinformatics

Gene Symbol DIAPH1
Entrez
Uniprot