![Western Blot: Connexin 26/GJB2 Antibody [NBP2-41304] - Western blotting analysis detects Cx26 using R(alpha)Cx26-Ct or R(alpha)Cx26-cl antibodies. (a) R(alpha)Cx26-Ct (A) and R(alpha)Cx26-cl (B) antibodies detected a specific 26kDa band on pig heart tissue lysates (lanes A and B are cropped blots obtained from the same gel). R(alpha)Cx26-cl recognized the 26kDa band on human and rat heart (H) lysates and on rat liver (L) sample (positive control) although two unspecific bands at molecular weights lower than 26kDa were detected. No bands were detected on smooth muscle (SM) tissue lysates (negative control) by R(alpha)Cx26-cl. R(alpha)GAPDH was used as protein loading control for rat tissue samples run on the same gel. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-018-32405-2), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Connexin-26-GJB2-Antibody-Western-Blot-NBP2-41304-img0005.jpg "Western Blot: Connexin 26/GJB2 Antibody [NBP2-41304] - Western blotting analysis detects Cx26 using R(alpha)Cx26-Ct or R(alpha)Cx26-cl antibodies. (a) R(alpha)Cx26-Ct (A) and R(alpha)Cx26-cl (B) antibodies detected a specific 26kDa band on pig heart tissue lysates (lanes A and B are cropped blots obtained from the same gel). R(alpha)Cx26-cl recognized the 26kDa band on human and rat heart (H) lysates and on rat liver (L) sample (positive control) although two unspecific bands at molecular weights lower than 26kDa were detected. No bands were detected on smooth muscle (SM) tissue lysates (negative control) by R(alpha)Cx26-cl. R(alpha)GAPDH was used as protein loading control for rat tissue samples run on the same gel. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-018-32405-2), licensed under a CC-BY license.")
| Reactivity | Hu, Rt, Po, MuSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Antibody was raised against a 16 amino acid peptide near the center of human GJB2. The immunogen is located within amino acids 80 - 130 of GJB2. Amino Acid Squence: HVAYRRHEKKRKFIKGE |
| Predicted Species | Mouse (94%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | GJB2 |
| Purity | Peptide affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Use in IHC-P reported in scientific literature (PMID:33171391) |
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| Theoretical MW | 26 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Peptide affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Connexin 26/GJB2 Antibody (NBP2-41304)Find related products by research area.
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| Gene Symbol | GJB2 |
![Immunocytochemistry/Immunofluorescence: Connexin 26/GJB2 Antibody [NBP2-41304] - Cx26 distribution at level of the cardiomyocyte cytoplasm. Confocal laser scanning microscopy: representative images of rat and pig heart sections. Double immunofluorescence analysis for Cx26 (red) and Cx43 (green) show a distinct cell localization of the two Cxs. (a) Three dimensional picture of the maximum intensity projection of the raw images of longitudinal rat heart section treated with R(alpha)Cx26-cl and M(alpha)Cx43. Scale bar: 20um. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-018-32405-2), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Connexin-26-GJB2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-41304-img0002.jpg "Immunocytochemistry/Immunofluorescence: Connexin 26/GJB2 Antibody [NBP2-41304] - Cx26 distribution at level of the cardiomyocyte cytoplasm. Confocal laser scanning microscopy: representative images of rat and pig heart sections. Double immunofluorescence analysis for Cx26 (red) and Cx43 (green) show a distinct cell localization of the two Cxs. (a) Three dimensional picture of the maximum intensity projection of the raw images of longitudinal rat heart section treated with R(alpha)Cx26-cl and M(alpha)Cx43. Scale bar: 20um. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-018-32405-2), licensed under a CC-BY license.")
![Immunohistochemistry: Connexin 26/GJB2 Antibody [NBP2-41304] - Human and rat cardiomyocytes immunoreact with R(alpha)Cx26 antibodies. Representative images of immunohistochemical analysis for Cx26 by R(alpha)Cx26-Ct or R(alpha)Cx26-cl antibodies. Longitudinal (c and d) sections showing positivity in cardiomyocytes and in smooth muscle cells of vessel (arrow); Rat (d) immunoperoxidase shows brown immunoreaction in the cell cytoplasm of working cardiomyocytes and in smooth muscle cells of vessels (arrows); positivity of cardiomyocytes and smooth muscle cells has shown, at higher magnification, in longitudinal (d) and cross sections (c); Negative controls are shown in the squares. Scale bars: 50um. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-018-32405-2), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Connexin-26-GJB2-Antibody-Immunohistochemistry-NBP2-41304-img0004.jpg "Immunohistochemistry: Connexin 26/GJB2 Antibody [NBP2-41304] - Human and rat cardiomyocytes immunoreact with R(alpha)Cx26 antibodies. Representative images of immunohistochemical analysis for Cx26 by R(alpha)Cx26-Ct or R(alpha)Cx26-cl antibodies. Longitudinal (c and d) sections showing positivity in cardiomyocytes and in smooth muscle cells of vessel (arrow); Rat (d) immunoperoxidase shows brown immunoreaction in the cell cytoplasm of working cardiomyocytes and in smooth muscle cells of vessels (arrows); positivity of cardiomyocytes and smooth muscle cells has shown, at higher magnification, in longitudinal (d) and cross sections (c); Negative controls are shown in the squares. Scale bars: 50um. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-018-32405-2), licensed under a CC-BY license.")
![Western Blot: Connexin 26/GJB2 Antibody [NBP2-41304] - Analysis of Connexin 26/GJB2 in human colon tissue lysate with Connexin 26/GJB2 antibody at (A) 1 and (B) 2 ug/ml.](http://images.novusbio.com/fullsize/Connexin-26-GJB2-Antibody-Western-Blot-NBP2-41304-img0001.jpg "Western Blot: Connexin 26/GJB2 Antibody [NBP2-41304] - Analysis of Connexin 26/GJB2 in human colon tissue lysate with Connexin 26/GJB2 antibody at (A) 1 and (B) 2 ug/ml.")
![Immunohistochemistry: Connexin 26/GJB2 Antibody [NBP2-41304] - Positive and negative tissue controls for Cx26. Representative images of immunohistochemical analysis for Cx26 on rat liver and skeletal muscle sections by R(alpha)Cx26-cl antibody. Cx26 positive brown punctate staining of hepatocyte plasma membrane (arrows) and cytoplasm (arrowheads) is shown in liver. By contrast, Cx26 staining is absent in skeletal muscle. Negative controls are showen in the squares. Scale bars: 50um. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-018-32405-2), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Connexin-26-GJB2-Antibody-Immunohistochemistry-NBP2-41304-img0003.jpg "Immunohistochemistry: Connexin 26/GJB2 Antibody [NBP2-41304] - Positive and negative tissue controls for Cx26. Representative images of immunohistochemical analysis for Cx26 on rat liver and skeletal muscle sections by R(alpha)Cx26-cl antibody. Cx26 positive brown punctate staining of hepatocyte plasma membrane (arrows) and cytoplasm (arrowheads) is shown in liver. By contrast, Cx26 staining is absent in skeletal muscle. Negative controls are showen in the squares. Scale bars: 50um. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-018-32405-2), licensed under a CC-BY license.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")