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![Western Blot: Connexin 26/GJB2 Antibody [NBP2-41304] - Western blotting analysis detects Cx26 using R(alpha)Cx26-Ct or R(alpha)Cx26-cl antibodies. (a) R(alpha)Cx26-Ct (A) and R(alpha)Cx26-cl (B) antibodies detected a specific 26kDa band on pig heart tissue lysates (lanes A and B are cropped blots obtained from the same gel). R(alpha)Cx26-cl recognized the 26kDa band on human and rat heart (H) lysates and on rat liver (L) sample (positive control) although two unspecific bands at molecular weights lower than 26kDa were detected. No bands were detected on smooth muscle (SM) tissue lysates (negative control) by R(alpha)Cx26-cl. R(alpha)GAPDH was used as protein loading control for rat tissue samples run on the same gel. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-018-32405-2), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Connexin-26-GJB2-Antibody-Western-Blot-NBP2-41304-img0005.jpg "Western Blot: Connexin 26/GJB2 Antibody [NBP2-41304] - Western blotting analysis detects Cx26 using R(alpha)Cx26-Ct or R(alpha)Cx26-cl antibodies. (a) R(alpha)Cx26-Ct (A) and R(alpha)Cx26-cl (B) antibodies detected a specific 26kDa band on pig heart tissue lysates (lanes A and B are cropped blots obtained from the same gel). R(alpha)Cx26-cl recognized the 26kDa band on human and rat heart (H) lysates and on rat liver (L) sample (positive control) although two unspecific bands at molecular weights lower than 26kDa were detected. No bands were detected on smooth muscle (SM) tissue lysates (negative control) by R(alpha)Cx26-cl. R(alpha)GAPDH was used as protein loading control for rat tissue samples run on the same gel. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-018-32405-2), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Connexin 26/GJB2 Antibody [NBP2-41304] - Cx26 distribution at level of the cardiomyocyte cytoplasm. Confocal laser scanning microscopy: representative images of rat and pig heart sections. Double immunofluorescence analysis for Cx26 (red) and Cx43 (green) show a distinct cell localization of the two Cxs. (a) Three dimensional picture of the maximum intensity projection of the raw images of longitudinal rat heart section treated with R(alpha)Cx26-cl and M(alpha)Cx43. Scale bar: 20um. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-018-32405-2), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Connexin-26-GJB2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-41304-img0002.jpg)
![Immunohistochemistry: Connexin 26/GJB2 Antibody [NBP2-41304] - Human and rat cardiomyocytes immunoreact with R(alpha)Cx26 antibodies. Representative images of immunohistochemical analysis for Cx26 by R(alpha)Cx26-Ct or R(alpha)Cx26-cl antibodies. Longitudinal (c and d) sections showing positivity in cardiomyocytes and in smooth muscle cells of vessel (arrow); Rat (d) immunoperoxidase shows brown immunoreaction in the cell cytoplasm of working cardiomyocytes and in smooth muscle cells of vessels (arrows); positivity of cardiomyocytes and smooth muscle cells has shown, at higher magnification, in longitudinal (d) and cross sections (c); Negative controls are shown in the squares. Scale bars: 50um. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-018-32405-2), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Connexin-26-GJB2-Antibody-Immunohistochemistry-NBP2-41304-img0004.jpg)
![Western Blot: Connexin 26/GJB2 Antibody [NBP2-41304] - Analysis of Connexin 26/GJB2 in human colon tissue lysate with Connexin 26/GJB2 antibody at (A) 1 and (B) 2 ug/ml.](http://images.novusbio.com/fullsize/Connexin-26-GJB2-Antibody-Western-Blot-NBP2-41304-img0001.jpg)
![Immunohistochemistry: Connexin 26/GJB2 Antibody [NBP2-41304] - Positive and negative tissue controls for Cx26. Representative images of immunohistochemical analysis for Cx26 on rat liver and skeletal muscle sections by R(alpha)Cx26-cl antibody. Cx26 positive brown punctate staining of hepatocyte plasma membrane (arrows) and cytoplasm (arrowheads) is shown in liver. By contrast, Cx26 staining is absent in skeletal muscle. Negative controls are showen in the squares. Scale bars: 50um. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-018-32405-2), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Connexin-26-GJB2-Antibody-Immunohistochemistry-NBP2-41304-img0003.jpg)
![Western Blot: Connexin 26/GJB2 Antibody [NB100-57840] - Staining of Mouse Brain lysate with antibody at 0.01 ug/mL (A), Mouse Heart (B) and Rat Heart (C) lysate with antibody at 0.5 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Connexin-26-GJB2-Antibody-Western-Blot-NB100-57840-img0002.jpg "Western Blot: Connexin 26/GJB2 Antibody [NB100-57840] - Staining of Mouse Brain lysate with antibody at 0.01 ug/mL (A), Mouse Heart (B) and Rat Heart (C) lysate with antibody at 0.5 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.")
![Flow Cytometry: Connexin 26/GJB2 Antibody [NB100-57840] - Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/Connexin-26-GJB2-Antibody-Flow-Cytometry-NB100-57840-img0003.jpg)
![Western Blot: Connexin 26/GJB2 Antibody [NB100-57840] - Staining of Human Colon (A), Heart (B), Prostate (C), Skin (D) and negative control Adipose (E) lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Connexin-26-GJB2-Antibody-Western-Blot-NB100-57840-img0001.jpg)
![ELISA: Human Connexin 26/GJB2 ELISA Kit (Colorimetric) [NBP2-79811] - Samples were spiked with high concentrations of Human Connexin 26/GJB2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-79811_human-connexin-26-gjb2-elisa-kit-colorimetric-132202416133648.jpg "ELISA: Human Connexin 26/GJB2 ELISA Kit (Colorimetric) [NBP2-79811] - Samples were spiked with high concentrations of Human Connexin 26/GJB2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human Connexin 26/GJB2 ELISA Kit (Colorimetric) [NBP2-79811] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-79811_human-connexin-26-gjb2-elisa-kit-colorimetric-432024924094.jpg)
![Western Blot: Connexin 26/GJB2 Antibody [NBP2-88789] - WB Suggested Anti-GJB2 Antibody Titration: 1.25ug/ml. ELISA Titer: 1:62500. Positive Control: Human Lung](http://images.novusbio.com/fullsize/Connexin-26-GJB2-Antibody-Western-Blot-NBP2-88789-img0001.jpg "Western Blot: Connexin 26/GJB2 Antibody [NBP2-88789] - WB Suggested Anti-GJB2 Antibody Titration: 1.25ug/ml. ELISA Titer: 1:62500. Positive Control: Human Lung")
![Western Blot: Connexin 26/GJB2 Antibody [NBP2-88789] - WB Suggested Anti-GJB2 Antibody. Positive Control: Lane 1: 4ug mCx26 elution fraction 6 Lane 2: 4ug mCx26 elution fraction 7Lane 3: 4ug mCx26 elution fraction 6 + other Cx26 antibody Lane 4: 4ug mCx26 elution fraction 7 + other Cx26 antibody. Primary A](http://images.novusbio.com/fullsize/Connexin-26-GJB2-Antibody-Western-Blot-NBP2-88789-img0002.jpg)
![ELISA: Rat Connexin 26/GJB2 ELISA Kit (Colorimetric) [NBP2-79812] - Samples were spiked with high concentrations of Rat Connexin 26/GJB2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-79812_rat-connexin-26-gjb2-elisa-kit-colorimetric-132202416235792.jpg "ELISA: Rat Connexin 26/GJB2 ELISA Kit (Colorimetric) [NBP2-79812] - Samples were spiked with high concentrations of Rat Connexin 26/GJB2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat Connexin 26/GJB2 ELISA Kit (Colorimetric) [NBP2-79812] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-79812_rat-connexin-26-gjb2-elisa-kit-colorimetric-43202495091.jpg)
![Western Blot: Connexin 26/GJB2 Antibody [NBP3-10835] - Western blot analysis of Connexin 26/GJB2 in Mouse Spleen lysates. Antibody dilution at 1ug/ml](http://images.novusbio.com/fullsize/Connexin-26-GJB2-Antibody-Western-Blot-NBP3-10835-img0001.jpg "Western Blot: Connexin 26/GJB2 Antibody [NBP3-10835] - Western blot analysis of Connexin 26/GJB2 in Mouse Spleen lysates. Antibody dilution at 1ug/ml")
![Western Blot: Connexin 26/GJB2 Overexpression Lysate (Adult Normal) [NBP2-04260] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Connexin 26/GJB2.](http://images.novusbio.com/fullsize/Connexin-26-GJB2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-04260-img0001.jpg "Western Blot: Connexin 26/GJB2 Overexpression Lysate (Adult Normal) [NBP2-04260] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Connexin 26/GJB2.")
![Sandwich ELISA: Connexin 26/GJB2 Antibody (1C6) [H00002706-M01] - Detection limit for recombinant GST tagged GJB2 is 3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Connexin-26-GJB2-Antibody-1C6-Sandwich-ELISA-H00002706-M01-img0001.jpg "Sandwich ELISA: Connexin 26/GJB2 Antibody (1C6) [H00002706-M01] - Detection limit for recombinant GST tagged GJB2 is 3 ng/ml as a capture antibody.")