FOXL2 Antibody

There are 2 forms of the blepharophimosis/ptosis/epicanthus inversus syndrome. In one form, called type I, eyelid abnormalities are associated with ovarian failure. In the second type, called type II, only the eyelid defects are found. Both types map to 3q23. Crisponi et al. (2001) refined the physical mapping of the BPES critical region and positionally cloned a novel, putative winged helix/forkhead transcription factor gene, FOXL2, in which they found mutations to produce truncated proteins in type I families and larger proteins in type II. Consistent with an involvement in those tissues, FOXL2 was selectively expressed in the mesenchyme of developing mouse eyelids and in adult ovarian follicles; in adult humans, it appeared predominantly in the ovary.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 5 confirmed species: Human, Mouse, Bovine, Chicken, Ferret.

We have publications tested in 11 applications: Chemotaxis, ICC/IF, IF/IHC, IHC, IHC-Fr, IHC-P, IHC-WhMt, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, WB.

Showing Publications 1 - 10 of 72. Show All 72 Publications. Collapse Publications.

Publications using NB100-1277	Applications	Species
Ting Zhao, Meina He, Zijian Zhu, Tuo Zhang, Wenying Zheng, Shaogang Qin, Meng Gao, Wenji Wang, Ziqi Chen, Jun Han, Longping Liu, Bo Zhou, Haibin Wang, Hua Zhang, Guoliang Xia, Jianbin Wang, Fengchao Wang, Chao Wang P62 promotes FSH-induced antral follicle formation by directing degradation of ubiquitinated WT1 Cellular and Molecular Life Sciences: CMLS 2024-05-20 [PMID: 38763964]
Lim J, Lee HL, Nguyen J et al. Adolescent exposure to low-dose ?9-tetrahydrocannabinol (THC) depletes the ovarian reserve in female mice Toxicological sciences : an official journal of the Society of Toxicology 2023-03-13 [PMID: 36912754] (IHC-Fr, Mouse) Details: 1:200 dilution	IHC-Fr	Mouse
Pierson Smela MD, Kramme CC, Fortuna PRJ et al. Directed differentiation of human iPSCs to functional ovarian granulosa-like cells via transcription factor overexpression eLife 2023-02-21 [PMID: 36803359] (ICC/IF, Human) Details: Dilution used in ICC/IF 1:250	ICC/IF	Human
Yu X, Wang N, Zhang Y et al. Oocyte Arrested at Metaphase II Stage Were Derived From Human Pluripotent Stem Cells in Vitro Research Square 2021-03-17 [PMID: 36735215] (ICC/IF, Human)	ICC/IF	Human
Cheng Y, Zhang Z, Zhang G et al. The Male-Biased Expression of miR-2954 Is Involved in the Male Pathway of Chicken Sex Differentiation Cells 2022-12-20 [PMID: 36611798] (IHC-Fr, Chicken)	IHC-Fr	Chicken
Zhang S, Mu L, Wang H et al. Quantitative proteomic analysis uncovers protein-expression profiles during gonadotropin-dependent folliculogenesis in mice Biology of reproduction 2022-12-08 [PMID: 36477298]
Bunce CM Reinterpreting the Organizing Principles of Sex Determination and Gonadogenesis in the Mouse Thesis 2021-01-01 (ICC/IF, Mouse)	ICC/IF	Mouse
Wang Y, Zhang J, Liang J et al. In vivo promotion of primordial follicle activation by stem cell factor treatment in mice with premature ovarian insufficiency and advanced age Molecular human reproduction 2022-12-07 [PMID: 36477300]
Lowe MG Mechanisms Regulating PGC Specification and Epigenomic Reprogramming Thesis 2022-01-01
Jasin Taelman, Sylwia M. Czukiewska, Ioannis Moustakas, Yolanda W. Chang, Sanne Hillenius, Talia van der Helm, Lotte E. van der Meeren, Hailiang Mei, Xueying Fan, Susana M. Chuva de Sousa Lopes Characterization of the human fetal gonad and reproductive tract by single-cell transcriptomics Developmental Cell 2024-02-26 [PMID: 38295793]
Baskin L, Cao M, Askel S et al. Ovotesticular cords and ovotesticular follicles: New markers in a model of human mixed ovotestis Differentiation; research in biological diversity 2023-11-19 [PMID: 38000942] (IHC, Human)	IHC	Human
Danti L, Lundin K, Sepponen K et al. CRISPR/Cas9-mediated activation of NR5A1 steers female human embryonic stem cell-derived bipotential gonadal-like cells towards a steroidogenic cell fate J Ovarian Res 2023-09-20 [PMID: 37726790]
Bridges K, Yao HH, Nicol B Loss of Runx1 Induces Granulosa Cell Defects and Development of Ovarian Tumors in the Mouse International journal of molecular sciences 2022-11-21 [PMID: 36430923] (ICC/IF, Mouse)	ICC/IF	Mouse
Overland M, Li Y, Derpinghaus A et al. Development of the human ovary: Fetal through pubertal ovarian morphology, folliculogenesis and expression of cellular differentiation markers Differentiation 2022-10-01 [PMID: 36347737] (IF/IHC, Human)	IF/IHC	Human
Thomson E, Tran M, Robevska G et al. Functional genomics analysis identifies loss of HNF1B function as a cause of Mayer-Rokitansky-K�ster-Hauser syndrome Human Molecular Genetics 2023-03-06 [PMID: 36282544] (Immunohistochemistry, Immunocytochemistry/ Immunofluorescence)	Immunohistochemistry, Immunocytochemistry/ Immunofluorescence
Del Valle JS, Mancini V, Laverde Garay M et al. Dynamic in vitro culture of cryopreserved-thawed human ovarian cortical tissue using a microfluidics platform does not improve early folliculogenesis Front Endocrinol (Lausanne) 2022-07-29 [PMID: 35966050]
Yu J, Xie X, Ma Y et al. Effects and potential mechanism of Ca2+/calmodulin dependent protein kinase II pathway inhibitor KN93 on the development of ovarian follicle International journal of molecular medicine 2022-10-01 [PMID: 35929517] (IHC-P, Mouse)	IHC-P	Mouse
Mizuta K, Katou Y, Nakakita B et al. Ex vivo reconstitution of fetal oocyte development in humans and cynomolgus monkeys The EMBO journal 2022-08-01 [PMID: 35912849]
Rodriguez KF, Brown PR, Amato CM et al. Somatic cell fate maintenance in mouse fetal testes via autocrine/paracrine action of AMH and activin B Nature communications 2022-07-15 [PMID: 35840551] (IHC-P, Mouse)	IHC-P	Mouse
Dai Y, Bo Y, Wang P et al. Asynchronous embryonic germ cell development leads to a heterogeneity of postnatal ovarian follicle activation and may influence the timing of puberty onset in mice BMC Biology 2022-12-01 [PMID: 35550124]
Li Y, Overland M, Derpinghaus A et al. Development of the human fetal testis: Morphology and expression of cellular differentiation markers Differentiation 2022-03-01 [PMID: 35490077] (IF/IHC, Human)	IF/IHC	Human
Du H, Guo Y, Wu X, Gong Y FOXL2 regulates the expression of the Col4a1 collagen gene in chicken granulosa cells Molecular reproduction and development 2022-02-01 [PMID: 35122350] (ICC/IF)	ICC/IF
Xu X, Mu L, Li L et al. Imaging and tracing the pattern of adult ovarian angiogenesis implies a strategy against female reproductive aging Science advances 2022-01-14 [PMID: 35020427] (IHC-P, Mouse)	IHC-P	Mouse
Guo Y, Cheng L, Li X Et al. Transcriptional regulation of CYP19A1 expression in chickens: ESR1, ESR2 and NR5A2 form a functional network General and comparative endocrinology 2021-10-27 [PMID: 34710471] (WB, IF/IHC, ICC/IF, Chicken)	WB, IF/IHC, ICC/IF	Chicken
Hammoud SS, Shen Y, LaRose H et al. Tcf21+ mesenchymal cells contribute to testis somatic cell development, homeostasis, and regeneration Nat Commun 2021-06-24 [PMID: 34162856]
Adeniran BV, Bjarkadottir BD, Appeltant R et al. Improved preservation of ovarian tissue morphology that is compatible with antigen detection using a fixative mixture of formalin and acetic acid Human reproduction (Oxford, England) 2021-05-06 [PMID: 33956944] (IF/IHC, Bovine, Human, Mouse)	IF/IHC	Bovine, Human, Mouse
Sasaki K, Oguchi A, Cheng K et al. The embryonic ontogeny of the gonadal somatic cells in mice and monkeys Cell reports 2021-05-04 [PMID: 33951437]
Ikeda Y, Tagami A, Maekawa M, Nagai A The conditional deletion of steroidogenic factor 1 (Nr5a1) in Sox9-Cre mice compromises testis differentiation Scientific reports 2021-02-24 [PMID: 33627800] (IHC-P, Mouse)	IHC-P	Mouse
Ruthig VA, Yokonishi T, Friedersdorf MB, et al. A transgenic DND1GFP fusion allele reports in vivo expression and RNA binding targets in undifferentiated mouse germ cells Biology of reproduction 2021-01-04 [PMID: 33394034]
Zhang J, Yan L, Wang Y et al. In vivo and in vitro activation of dormant primordial follicles by EGF treatment in mouse and human Clinical and translational medicine 2020-09-01 [PMID: 32997412] (IF/IHC, Human)	IF/IHC	Human
Yusuke Murase, Yukihiro Yabuta, Hiroshi Ohta, Chika Yamashiro, Tomonori Nakamura, Takuya Yamamoto, Mitinori Saitou Long-term expansion with germline potential of human primordial germ cell-like cells in vitro. The EMBO journal 2021-04-19 [PMID: 32954504]
Nicol B, Rodriguez K, Yao HH. Aberrant and constitutive expression of FOXL2 impairs ovarian development and functions in mice Biology of Reproduction 2020-10-29 [PMID: 32945847] (Immunohistochemistry-Frozen, Immunocytochemistry/ Immunofluorescence)	Immunohistochemistry-Frozen, Immunocytochemistry/ Immunofluorescence
Li YH, Chen TM, Huang BM et al. FGF9 is a downstream target of SRY and sufficient to determine male sex fate in ex vivo XX gonad culture Biol. Reprod. 2020-09-04 [PMID: 32886743] (WB)	WB
Richardson N, Gillot I, Gregoire EP et al. Sox8 and Sox9 act redundantly for ovarian-to-testicular fate reprogramming in the absence of R-spondin1 in mouse sex reversals Elife 2020-05-26 [PMID: 32450947] (ICC/IF, Mouse)	ICC/IF	Mouse
De Cian MC, Gregoire EP, Le Rolle M et al. R-spondin2 signaling is required for oocyte-driven intercellular communication and follicular growth Cell Death Differ. 2020-04-27 [PMID: 32341451] (IHC-P, IF/IHC, Mouse)	IHC-P, IF/IHC	Mouse
Yamashiro C, Sasaki K, Yokobayashi S et al. Generation of human oogonia from induced pluripotent stem cells in culture Nat Protoc 2020-04-01 [PMID: 32231324]
FOXO1 negates the cooperative action of FOXL2C134W and SMAD3 in CYP19 expression in HGrC1 cells by sequestering SMAD3. Belli, M;Secchi, C;Stupack J Endocr Soc [PMID: 31701078] (WB, Human)	WB	Human
Garcia-Moreno SA, Lin YT, Futtner CR, et al. CBX2 is required to stabilize the testis pathway by repressing Wnt signaling PLoS Genet. 2019-05-22 [PMID: 31116734] (ICC/IF, Mouse)	ICC/IF	Mouse
Fujisawa M, Moh-Moh-Aung A, Zeng Z et al. Ovarian stromal cells as a source of cancer-associated fibroblasts in human epithelial ovarian cancer: A histopathological study. PLoS ONE. 2018-10-10 [PMID: 30304016] (IHC-P, Human)	IHC-P	Human
Nicol B, Grimm SA, Gruzdev A et al. Genome-wide identification of FOXL2 binding and characterization of FOXL2 feminizing action in the fetal gonads. Hum. Mol. Genet. 2018-09-13 [PMID: 30212841] (Chemotaxis, Mouse)	Chemotaxis	Mouse
Liu C, Rodriguez KF, Brown PR, Yao HH. Reproductive, physiological, and molecular outcomes in female mice deficient in Dhh and Ihh Endocrinology 2018-05-16 [PMID: 29788357] (IF/IHC, Mouse)	IF/IHC	Mouse
Belli M, Iwata N, Nakamura T et al. FOXL2C134W-induced CYP19 expression via cooperation with SMAD3 in HGrC1 cells. Endocrinology 2018-02-19 [PMID: 29471425] (Mouse)		Mouse
Abe A, Ohishi Y, Miyazaki T, Ozono K et al. Microcystic pattern should be recognized as part of the morphologic spectrum of solid-pseudopapillary neoplasm of the pancreas Histopathology 2017-08-31 [PMID: 28858381] (Human)		Human
Gonen N, Quinn A, O'Neill HC et al. Normal Levels of Sox9 Expression in the Developing Mouse Testis Depend on the TES/TESCO Enhancer, but This Does Not Act Alone PLoS Genet 2017-01-01 [PMID: 28045957] (ICC/IF, Mouse)	ICC/IF	Mouse
Ikeda Y, Tagami A, Komada M, Takahashi M. Expression of Kisspeptin in Gonadotrope Precursors in the Mouse Pituitary during Embryonic and Postnatal Development and in Adulthood. Neuroendocrinology. 2016-11-21 [PMID: 27871073]
Hikabe O, Hamazaki N, Nagamatsu G et al. Reconstitution in vitro of the entire cycle of the mouse female germ line. Nature 2016-10-17 [PMID: 27750280] (IF/IHC, Mouse)	IF/IHC	Mouse
Batchvarov IS, Taylor RW, Bustamante-Marin X et al. A grafted ovarian fragment rescues host fertility after chemotherapy. Mol. Hum. Reprod. 2016-10-03 [PMID: 27698028] (ICC/IF, Mouse)	ICC/IF	Mouse
Dorner J, Martinez Rodriguez V, Ziegler R et al. GLI1(+) progenitor cells in the adrenal capsule of the adult mouse give rise to heterotopic gonadal-like tissue Mol. Cell. Endocrinol. 2016-08-29 [PMID: 27585489] (IF/IHC, ICC/IF, Mouse)	IF/IHC, ICC/IF	Mouse
Arora R, Abby E, Ross AD et al. Meiotic onset is reliant on spatial distribution but independent of germ cell number in the mouse ovary. J Cell Sci. 2016-05-19 [PMID: 27199373] (ICC/IF, Mouse)	ICC/IF	Mouse
Feng L, Wang Y, Cai H et al. ADAM10-Notch signaling governs the recruitment of ovarian pregranulosa cells and controls folliculogenesis in mice. J. Cell. Sci. 2016-04-15 [PMID: 27084580] (IHC-P, Mouse)	IHC-P	Mouse
Croce S, de Kock L, Boshari T et al. Uterine Tumor Resembling Ovarian Sex Cord Tumor (UTROSCT) Commonly Exhibits Positivity With Sex Cord Markers FOXL2 and SF-1 but Lacks FOXL2 and DICER1 Mutations. Int. J. Gynecol. Pathol. 2015-11-23 [PMID: 26598979] (IHC-P, Human)	IHC-P	Human
Barratt CL A grafted ovarian fragment rescues host fertility after chemotherapy Mol Hum Reprod. 2016-10-11 [PMID: 26590382] (ICC/IF, Mouse)	ICC/IF	Mouse
Spectrum WM. Juvenile Granulosa Cell Tumors of the Testis: A Clinicopathologic Study of 70 Cases With Emphasis on Its Wide Morphologic Spectrum American Journal of Surgical Pathology. 2015-06-05 [PMID: 26076062] (IHC-P, Human)	IHC-P	Human
Teng Z, Wang C, Wang Y et al. S100A8, an Oocyte-Specific Chemokine, Directs the Migration of Ovarian Somatic Cells During Mice Primordial Follicle Assembly. J. Cell. Physiol. 2015-05-06 [PMID: 25953201] (IHC-P, Mouse) Details: FOXL2 antibody (Imgenex IMG-3228) was used for IHC-P analysis of FOXL2 protein (as pre-granulosa cell specific marker) in tissue structures / reconstructed follicular-like cell masses which were created from murine ovarian cells. The immuno assay involved - 4% PFA fixation, 5um paraffin sections, antigen retrieval via boiling in 0.01% sodium citrate buffer, use of FOXL2 primary antibody at 1:100 dilution with 4C ON incubation, detection with IF labelled secondary (Fig. 1C).	IHC-P	Mouse
Schillebeeckx M, Pihlajoki M, Gretzinger E et al. Novel markers of gonadectomy-induced adrenocortical neoplasia in the mouse and ferret. Mol. Cell. Endocrinol. 2014-10-05 [PMID: 25289806] (IHC-P, Ferret, Mouse) Details: FOXL2 antibody used for IHC-P on adrenocortical tumor tissues obtained from mouse and ferrets gonadectomy-induced adrenocortical neoplasia model - 4% PFA ON fixation, paraffin embedding, 5um sections, primary used at 1:400 dilution, donkey anti-goat biotinylated IgG secondary, avidin-biotin immunoperoxidase system - DAB detection, no primary negative controls (Staining images -Fig?4, Fig?5, Fig S1).	IHC-P	Ferret, Mouse
Kao C S, Ulbright T M et al. Gonadoblastoma: an immunohistochemical study and comparison to Sertoli cell nodule with intratubular germ cell neoplasia, with pathogenetic implications. Histopathology 2014-01-12 [PMID: 24766183] (IHC-P, Human)	IHC-P	Human
Arora R, Altman E, Tran ND et al. Novel domains of expression for orphan receptor tyrosine kinase Ror2 in the human and mouse reproductive system. Dev. Dyn. 2014-04-18 [PMID: 24753105] (IHC-WhMt, Human)	IHC-WhMt	Human
The Expression of FOXL2 in Pancreatic, Hepatobiliary, and Renal Tumors with Ovarian-Type Stroma. Westerhoff M, Tretiakova M, Hart J et al. Hum Pathol [PMID: 24746205] (IHC-P, Human)	IHC-P	Human
Umeda S, Tateno M, Miyagi E et al. Uterine tumors resembling ovarian sex cord tumors (UTROSCT) with metastasis: clinicopathological study of two cases. Int J Clin Exp Pathol 2014-04-03 [PMID: 24696722] (IHC-P, Human) Details: Ovarian sex cord tumor: Fig 3C, Table 2.	IHC-P	Human
Alekseeva, L;Laman, A;Shepelyakovskaya, A;Plekhanova, N;Ivanov, V; Inhibitory Effect of Transcription Factor YB-1 in the Development of Signal from an Innate Immunity Receptor NOD2 Russ J Bioorg Chem Jul 1 2019
Anttonen M, Pihlajoki M, Andersson N et al. FOXL2, GATA4, and SMAD3 co-operatively modulate gene expression, cell viability and apoptosis in ovarian granulosa cell tumor cells. PLoS ONE. 2014-01-13 [PMID: 24416423] (WB, IHC-P, Human) Details: WB: FOXL2-transfected COS-7 and COV434 cells, Fig 1. IHC(P): Proliferating ovarian follicles (Fig 4) and granulosa cell tumors (Tables 1,2). Notes: The specificity of the FOXL2 pAb was validated in FOXL2 transfected cells by WB (Fig1). In IHC(P), FOXL2 wa	WB, IHC-P	Human
Kommoss S, Gilks CB, Penzel R et al. A current perspective on the pathological assessment of FOXL2 in adult-type granulosa cell tumours of the ovary. Histopathology. 2014-02-01 [PMID: 24192202] (IHC-P, Human)	IHC-P	Human
Maillet D, Goulvent T, Rimokh R et al. Impact of a second opinion using expression and molecular analysis of FOXL2 for sex cord-stromal tumors. A study of the GINECO group & the TMRO network. Gynecol Oncol 2013-10-22 [PMID: 24157616] (IHC-P, Human)	IHC-P	Human
Maatouk DM, Mork L, Hinson A et al. Germ cells are not required to establish the female pathway in mouse fetal gonads PLoS One 2012-01-01 [PMID: 23091613] (IHC-Fr, ICC/IF, Mouse)	IHC-Fr, ICC/IF	Mouse
Jameson SA, Lin YT, Capel B. Testis development requires the repression of Wnt4 by Fgf signaling Dev Biol 2012-06-13 [PMID: 22705479] (ICC/IF, IF/IHC, Mouse)	ICC/IF, IF/IHC	Mouse
Mork L, Maatouk DM, McMahon JA et al. Temporal Differences in Granulosa Cell Specification in the Ovary Reflect Distinct Follicle Fates in Mice. Biology of Reproduction. 2011-10-05 [PMID: 21976597]
Al-Agha OM, Huwait HF, Chow C et al. FOXL2 is a sensitive and specific marker for sex cord-stromal tumors of the ovary. Am J Surg Pathol. 2011-04-01 [PMID: 21378549] (IHC-P) Details: IHC (p): Human ovarian cell tumors, Tables 1,2 and Figs 1-4. Note: The FOXL2/BPES IMG-3228 antibody was used at a dilution of 1:25 (20 ug/ml). FOXL2 protein expression was identified as a sensitive and specific marker for sex cord-stromal ovarian tumors (	IHC-P
Pulinilkunnil T, He H, Kong D et al. Adrenergic regulation of AMP-activated protein kinase in brown adipose tissue in vivo. J Biol Chem. 2011-03-18 [PMID: 21209093] Details: WB: GAPDH Loading Control (IMG-3073). Mouse interscapular brown adipose cells, Fig 3A.
Lamba P, Wang Y, Tran S et al. Activin A regulates porcine follicle-stimulating hormone beta-subunit transcription via cooperative actions of SMADs and FOXL2. Endocrinology. 2010-11-01 [PMID: 20810560] (WB, Mouse) Details: FOXL2/BPES (IMG-3228). WB: Immortalized murine gonadotrope (LbetaT2) cell line, Fig 2B.	WB	Mouse
Jamieson S, Butzow R, Andersson N et al. The FOXL2 C134W mutation is characteristic of adult granulosa cell tumors of the ovary. Mod Pathol. 2010-11-01 [PMID: 20693978] (Human) Details: IHC (p): Human ovarian granulosa cell tumors, Fig 1. Note: The FOXL2/BPES IMG-3228 antibody was used at a dilution of 1:400 (1.25 ug/ml). Both a diffuse and differentiated adult granulosa cell tumor had strong nuclear positivity of FOXL2. Another presuppo		Human
Blount AL, Schmidt K, Justice NJ et al. FoxL2 and Smad3 coordinately regulate follistatin gene transcription. J Biol Chem. 2009-03-20 [PMID: 19106105] (IHC-Fr, WB, Chemotaxis, Mouse) Details: Mouse gonadotrope-derived alphaT3-1, WB: Figs. 4, 5a,b,c, 6a,b, 11a,b,d; ChIP: Fig. 5b; IHC (frozen): Fig. 3a,d,g.	IHC-Fr, WB, Chemotaxis	Mouse
Crisponi L, Deiana M, Loi A et al. The putative forkhead transcription factor FOXL2 is mutated in blepharophimosis/ptosis/epicanthus inversus syndrome. Nat Genet 2001-02-01 [PMID: 11175783]
Show All 72 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
Enlarge	5 reviewed by: Ripla Arora	IF	Mouse	07/17/2015	View Summary Application Immunofluorescence Sample Tested Mouse Ovary Species Mouse

Array NB100-1277

• FOXL2 Antibodies
• FOXL2 Lysate
• FOXL2 Proteins