![Western Blot: Splicing Factor 1 Antibody (2E12) [H00007536-M01] - Analysis of SF1 expression in transfected 293T cell line by SF1 monoclonal antibody (M01), clone 2E12.Lane 1: SF1 transfected lysate (Predicted MW: 59.7 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Splicing-Factor-1-Antibody-2E12-Western-Blot-H00007536-M01-img0012.jpg "Western Blot: Splicing Factor 1 Antibody (2E12) [H00007536-M01] - Analysis of SF1 expression in transfected 293T cell line by SF1 monoclonal antibody (M01), clone 2E12.Lane 1: SF1 transfected lysate (Predicted MW: 59.7 KDa).Lane 2: Non-transfected lysate.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC, IP |
| Clone | 2E12 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Description | Quality control test: Antibody Reactive Against Recombinant Protein. |
| Immunogen | SF1 (NP_004621, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MATGANATPLDFPSKKRKRSRWNQDTMEQKTVIPGMPTVIPPGLTREQERAYIVQLQIEDLTRKLRTGDLGIPPNPEDRSPSPEPIYNSEGKRLNTREFRTRKKLEEERH |
| Specificity | SF1 - splicing factor 1 |
| Isotype | IgG2a Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | SF1 |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Antibody reactivity against recombinant protein for WB. It has been used for IHC-P, IF and ELISA. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | IgG purified |
Secondary Antibodies |
Isotype Controls |
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![Immunocytochemistry/Immunofluorescence: Splicing Factor 1 Antibody (2E12) [H00007536-M01] - Analysis of monoclonal antibody to SF1 on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Splicing-Factor-1-Antibody-2E12-Immunocytochemistry-Immunofluorescence-H00007536-M01-img0005.jpg "Immunocytochemistry/Immunofluorescence: Splicing Factor 1 Antibody (2E12) [H00007536-M01] - Analysis of monoclonal antibody to SF1 on HeLa cell . Antibody concentration 10 ug/ml.")
![Immunohistochemistry-Paraffin: Splicing Factor 1 Antibody (2E12) [H00007536-M01] - Analysis of monoclonal antibody to SF1 on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/Splicing-Factor-1-Antibody-2E12-Immunohistochemistry-Paraffin-H00007536-M01-img0007.jpg "Immunohistochemistry-Paraffin: Splicing Factor 1 Antibody (2E12) [H00007536-M01] - Analysis of monoclonal antibody to SF1 on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml.")
![Western Blot: Splicing Factor 1 Antibody (2E12) [H00007536-M01] - SF1 monoclonal antibody (M01), clone 2E12. Analysis of SF1 expression in HeLa.](http://images.novusbio.com/fullsize/Splicing-Factor-1-Antibody-2E12-Western-Blot-H00007536-M01-img0010.jpg "Western Blot: Splicing Factor 1 Antibody (2E12) [H00007536-M01] - SF1 monoclonal antibody (M01), clone 2E12. Analysis of SF1 expression in HeLa.")
![Western Blot: Splicing Factor 1 Antibody (2E12) [H00007536-M01] - SF1 monoclonal antibody (M01), clone 2E12. Analysis of SF1 expression in NIH/3T3.](http://images.novusbio.com/fullsize/Splicing-Factor-1-Antibody-2E12-Western-Blot-H00007536-M01-img0011.jpg "Western Blot: Splicing Factor 1 Antibody (2E12) [H00007536-M01] - SF1 monoclonal antibody (M01), clone 2E12. Analysis of SF1 expression in NIH/3T3.")
![Western Blot: Splicing Factor 1 Antibody (2E12) [H00007536-M01] - Analysis of SF1 expression in transfected 293T cell line by SF1 monoclonal antibody (M01A), clone 2E12.Lane 1: SF1 transfected lysate (Predicted MW: 59.7 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Splicing-Factor-1-Antibody-2E12-Western-Blot-H00007536-M01-img0013.jpg "Western Blot: Splicing Factor 1 Antibody (2E12) [H00007536-M01] - Analysis of SF1 expression in transfected 293T cell line by SF1 monoclonal antibody (M01A), clone 2E12.Lane 1: SF1 transfected lysate (Predicted MW: 59.7 KDa).Lane 2: Non-transfected lysate.")
![Immunoprecipitation: Splicing Factor 1 Antibody (2E12) [H00007536-M01] - Analysis of SF1 transfected lysate using anti-SF1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with SF1 monoclonal antibody.](http://images.novusbio.com/fullsize/Splicing-Factor-1-Antibody-2E12-Immunoprecipitation-H00007536-M01-img0008.jpg "Immunoprecipitation: Splicing Factor 1 Antibody (2E12) [H00007536-M01] - Analysis of SF1 transfected lysate using anti-SF1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with SF1 monoclonal antibody.")
![ELISA: Splicing Factor 1 Antibody (2E12.) [H00007536-M01] - Splicing Factor 1 Antibody (2E12) [H00007536-M01] - Detection limit for recombinant GST tagged SF1 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Splicing-Factor-1-Antibody-2E12-Sandwich-ELISA-H00007536-M01-img0009.jpg "ELISA: Splicing Factor 1 Antibody (2E12.) [H00007536-M01] - Splicing Factor 1 Antibody (2E12) [H00007536-M01] - Detection limit for recombinant GST tagged SF1 is approximately 0.03ng/ml as a capture antibody.")
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