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SF2 Antibody

Images

 
Western Blot: SF2 Antibody [NBP2-20324] - Rat tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with SF2 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody ...read more
Immunocytochemistry/ Immunofluorescence: SF2 Antibody [NBP2-20324] - Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa, using SF2 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled ...read more
Immunohistochemistry-Paraffin: SF2 Antibody [NBP2-20324] - Paraffin-embedded mouse duodenum. SF2 antibody dilution: 1:500.
Western Blot: SF2 Antibody [NBP2-20324] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 12% SDS PAGE gel, diluted at 1:1000.
Western Blot: SF2 Antibody [NBP2-20324] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with SF2 antibody diluted at 1:5000.
Immunohistochemistry-Paraffin: SF2 Antibody [NBP2-20324] - Antibody detects SFRS1 protein at nucleus on BT483 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded BT483 xenograft. Antibody dilution: ...read more
Immunohistochemistry-Paraffin: SF2 Antibody [NBP2-20324] - Antibody detects SFRS1 protein at nucleus on colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. Antibody dilution: ...read more
Immunohistochemistry-Paraffin: SF2 Antibody [NBP2-20324] - Paraffin-embedded mouse skin. SF2 antibody dilution: 1:500.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ch, Pm, RMSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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SF2 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human SF2. The exact sequence is proprietary.
Localization
Cytoplasm; Nucleus speckle
Predicted Species
Porcine (100%), Bovine (100%), Rhesus Macaque (100%), Chicken (98%), Primate (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SRSF1
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:10000
Theoretical MW
28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using NBP2-20324.

Reactivity Notes

Chimpanzee (100%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for SF2 Antibody

  • Alternative-splicing factor 1
  • ASFpre-mRNA-splicing factor SF2, P33 subunit
  • MGC5228
  • serine/arginine-rich splicing factor 1
  • SF2FLJ53078
  • SF2p33
  • SFRS1splicing factor 2
  • Splicing factor, arginine/serine-rich 1ASF-1
  • SR splicing factor 1
  • SRp30a

Background

This gene encodes a member of the arginine/serine-rich splicing factor protein family, and functions in both constitutive and alternative pre-mRNA splicing. The protein binds to pre-mRNA transcripts and components of the spliceosome, and can either activate or repress splicing depending on the location of the pre-mRNA binding site. The protein's ability to activate splicing is regulated by phosphorylation and interactions with other splicing factor associated proteins. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for SF2 Antibody (NBP2-20324)(2)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SF2 Antibody (NBP2-20324) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SRSF1
Uniprot