![Western Blot: SRp55 Antibody [NBP2-04142] - CEM-T4 and HeLa whole cell lysates. Image from verified customer review.](http://images.novusbio.com/fullsize/SRp55-Antibody-Western-Blot-NBP2-04142-img0013.jpg "Western Blot: SRp55 Antibody [NBP2-04142] - CEM-T4 and HeLa whole cell lysates. Image from verified customer review.")
| Reactivity | Hu, Mu, BvSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1.0 mg/ml |
| Immunogen | The immunogen recognized by this antibody maps to this antibody using the numbering given in entry NP_006266.2 (GeneID 6431). |
| Predicted Species | Bovine (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | SRSF6 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. SRp55 antibody validated for ICC/IF from a verified customer review. |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
| Images | Ratings | Applications | Species | Date | Details | ||||||||||
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reviewed by:
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WB | Human | 06/09/2018 |
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reviewed by:
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ICC | Human | 11/18/2017 |
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reviewed by:
Verified Customer |
WB | Human | 01/21/2016 |
Summary
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Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
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| Verified Customer 06/09/2018 |
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| Application: | WB | |
| Species: | Human |
| Verified Customer 11/18/2017 |
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| Application: | ICC | |
| Species: | Human |
| Verified Customer 01/21/2016 |
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| Application: | WB | |
| Species: | Human |
| Gene Symbol | SRSF6 |
| Uniprot |
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![Immunocytochemistry/Immunofluorescence: SRp55 Antibody [NBP2-04142] - Staining in human fibroblasts. 1X PBS/5% normal serum/0.3% Triton™ X-100. Diluted 1:200 in 1X PBS/1% BSA/0.3% Triton™ X-100, 16 hours at 4C incubation. 2 hours (25C) secondary (1.5:1000 dilution). Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/SRp55-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-04142-img0014.jpg "Immunocytochemistry/Immunofluorescence: SRp55 Antibody [NBP2-04142] - Staining in human fibroblasts. 1X PBS/5% normal serum/0.3% Triton™ X-100. Diluted 1:200 in 1X PBS/1% BSA/0.3% Triton™ X-100, 16 hours at 4C incubation. 2 hours (25C) secondary (1.5:1000 dilution). Image submitted by a verified customer review.")
![Immunohistochemistry-Paraffin: SRp55 Antibody [NBP2-04142] - Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti- SRp55 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).](http://images.novusbio.com/fullsize/SRp55-Antibody-Immunohistochemistry-NBP2-04142-img0012.jpg "Immunohistochemistry-Paraffin: SRp55 Antibody [NBP2-04142] - Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti- SRp55 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).")
![Western Blot: SRp55 Antibody [NBP2-04142] - analysis of SRp55 in human lymphocytes using anti-SRp55 antibody. Each lane loaded with 14ug of whole cell lysate with 3 different human B-cell lines. Image from verified customer review.](http://images.novusbio.com/fullsize/SRp55-Antibody-Western-Blot-NBP2-04142-img0010.jpg "Western Blot: SRp55 Antibody [NBP2-04142] - analysis of SRp55 in human lymphocytes using anti-SRp55 antibody. Each lane loaded with 14ug of whole cell lysate with 3 different human B-cell lines. Image from verified customer review.")
![Immunohistochemistry-Paraffin: SRp55 Antibody [NBP2-04142] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- SRp55 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).](http://images.novusbio.com/fullsize/SRp55-Antibody-Immunohistochemistry-NBP2-04142-img0011.jpg "Immunohistochemistry-Paraffin: SRp55 Antibody [NBP2-04142] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- SRp55 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).")
![Immunoprecipitation: SRp55 Antibody [NBP2-04142] - Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), Jurkat (J; 50 ug) and mouse NIH3T3 (M; 50 ug) cells.Antibodies: Affinity purified rabbit anti-SRp55 antibody NBP2-04142 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. SRp55 was also immunoprecipitated by rabbit anti-SRp55 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).](http://images.novusbio.com/fullsize/SRp55-Antibody-Immunoprecipitation-NBP2-04142-img0002.jpg "Immunoprecipitation: SRp55 Antibody [NBP2-04142] - Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), Jurkat (J; 50 ug) and mouse NIH3T3 (M; 50 ug) cells.Antibodies: Affinity purified rabbit anti-SRp55 antibody NBP2-04142 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. SRp55 was also immunoprecipitated by rabbit anti-SRp55 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
