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CYP11A1 Antibody

Images

 
Orthogonal Strategies: Immunohistochemistry-Paraffin: CYP11A1 Antibody [NBP1-85368] - Staining in human adrenal gland and cerebral cortex tissues using anti-CYP11A1 antibody. Corresponding CYP11A1 RNA-seq data ...read more
Western Blot: CYP11A1 Antibody [NBP1-85368] - protein extract from: 1) human liver (negative), 2) human testis (positve), 3) MW marker (drawn with special ECL reagent marker), 4) human testis, 5) H295R adrenal cell ...read more
Immunocytochemistry/ Immunofluorescence: CYP11A1 Antibody [NBP1-85368] - CYP11A1 expression (Leydig cells) present in early pubertal Rhesus monkey. Secondary antibody: Alexa Fluor488 anti rabbit. Image submitted by a ...read more
Immunohistochemistry-Paraffin: CYP11A1 Antibody [NBP1-85368] - Staining of human cerebral cortex shows low expression as expected.
Immunohistochemistry-Paraffin: CYP11A1 Antibody [NBP1-85368] - Staining of human adrenal gland shows high expression.

Product Details

Summary
Reactivity Hu, RMSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

CYP11A1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: RSVLVKGCQTFLSAPREGLGRLRVPTGEGAGISTRSPRPFNEIPSPGDNGWLNLYHFWRETGTHKVHLHHVQNFQKYGPIYREKLGNVESVYVIDPEDVALLFKSEGPNPERFLIPPWVAYHQYYQRPIGVLLKKSAA
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CYP11A1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. CYP11A1 antibody validated for ICC/IF, WB from verified customer reviews.
Control Peptide
CYP11A1 Protein (NBP1-85368PEP)
Reviewed Applications
Read 4 Reviews rated 5
using
NBP1-85368 in the following applications:

Publications
Read Publications using
NBP1-85368 in the following applications:

Reactivity Notes

Rhesus monkey reactivity reported from a verified customer review.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for CYP11A1 Antibody

  • Cholesterol desmolase
  • cholesterol monooxygenase (side-chain-cleaving)
  • cholesterol side-chain cleavage enzyme, mitochondrial
  • CYPXIA1
  • Cytochrome P450 11A1
  • Cytochrome P450(scc)
  • cytochrome P450, family 11, subfamily A, polypeptide 1
  • cytochrome P450C11A1
  • EC 1.14.15
  • EC 1.14.15.6
  • steroid 20-22-lyase
  • subfamily XIA (cholesterol side chain cleavage)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ICC/IF, IHC

Publications for CYP11A1 Antibody (NBP1-85368)(3)

Reviews for CYP11A1 Antibody (NBP1-85368) (4) 54

Average Rating: 5
(Based on 4 reviews)
We have 4 reviews tested in 2 species: Human, Rhesus Macaque.

Reviews using NBP1-85368:
Filter by Applications
Immunofluorescence-Paraffin
(1)
IHC-P
(2)
WB
(1)
All Applications
Filter by Species
Human
(2)
Rhesus Macaque
(2)
All Species
Images Ratings Applications Species Date Details
Other CYP11A1 NBP1-85368
Enlarge
5
reviewed by:
Paula Aliberti
Immunofluorescence-Paraffin Rhesus Macaque 03/09/2018
View

Summary

ApplicationOther
Sample TestedEarly pubertal testis
SpeciesRhesus Macaque
LotB106749

Comments

CommentsPrimary antibody dilution 1:250.
Secondary antibody used: Alexa Fluor 488 anti rabbit
Immunohistochemistry-Paraffin CYP11A1 NBP1-85368
Enlarge
5
reviewed by:
Paula Aliberti
IHC-P Rhesus Macaque 11/24/2017
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedpubertal testis
SpeciesRhesus Macaque
LotB106749
Immunohistochemistry-Paraffin CYP11A1 NBP1-85368
Enlarge
5
reviewed by:
Paula Aliberti
IHC-P Human 10/09/2017
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedHuman testis
SpeciesHuman
LotB106749

Comments

CommentsAntibody diluted 1/250
Western Blot CYP11A1 NBP1-85368
Enlarge
5
reviewed by:
Paula Aliberti
WB Human 10/09/2017
View

Summary

ApplicationWestern Blot
Sample TestedHuman liver,Human testis,Whole cell lysate and mitochondrial extract of H295R cells (human adrenal cortical cells)
SpeciesHuman
LotB106749

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CYP11A1 Antibody (NBP1-85368) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional CYP11A1 Products

Research Areas for CYP11A1 Antibody (NBP1-85368)

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Blogs on CYP11A1

There are no specific blogs for CYP11A1, but you can read our latest blog posts.
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Recent Reviews

5
5
4
4
0
3
0
2
0
1
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Paula Aliberti
03/09/2018
Application: Immunofluorescence-Paraffin
Species: Rhesus Macaque

Paula Aliberti
11/24/2017
Application: IHC-P
Species: Rhesus Macaque

Paula Aliberti
10/09/2017
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol CYP11A1