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CYBB/NOX2 Antibody (NL7) [PE/Cy5.5]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC
Clone
NL7
Clonality
Monoclonal
Host
Mouse
Conjugate
PE/Cy5.5

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-41012AF350
Alexa Fluor 405
NBP1-41012AF405
Alexa Fluor 488
NBP1-41012AF488
Alexa Fluor 532
NBP1-41012AF532
Alexa Fluor 594
NBP1-41012AF594
Alexa Fluor 647
NBP1-41012AF647
Alexa Fluor 700
NBP1-41012AF700
Alexa Fluor 750
NBP1-41012AF750
Allophycocyanin
NBP1-41012APC
Allophycocyanin/Cy7
NBP1-41012APCCY7
Biotin
NBP1-41012B
CoraFluor 1
NBP1-41012CL1
DyLight 350
NBP1-41012UV
DyLight 405
NBP1-41012V
DyLight 488
NBP1-41012G
DyLight 550
NBP1-41012R
DyLight 594
NBP1-41012DL594
DyLight 650
NBP1-41012C
DyLight 680
NBP1-41012FR
DyLight 755
NBP1-41012IR
FITC
NBP1-41012F
HRP
NBP1-41012H
Janelia Fluor 525
NBP1-41012JF525
Janelia Fluor 549
NBP1-41012JF549
Janelia Fluor 585
NBP1-41012JF585
Janelia Fluor 635
NBP1-41012JF635
Janelia Fluor 646
NBP1-41012JF646
Janelia Fluor 669
NBP1-41012JF669
PE
NBP1-41012PE
PE/Atto594
NBP1-41012PEATT594
PE/Cy7
NBP1-41012PECY7
PerCP
NBP1-41012PCP
Unconjugated
NBP1-41012-0.1mg
mFluor Violet 450 SE
NBP1-41012MFV450
mFluor Violet 500 SE
NBP1-41012MFV500
mFluor Violet 610 SE
NBP1-41012MFV610
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CYBB/NOX2 Antibody (NL7) [PE/Cy5.5] Summary

Immunogen
Cytochrome B245 solubilized in octyl-beta-glucopyranoside
Epitope
Clone NL7 binds amino acids 498-506 (EKDVITGLK), a cytosolic epitope on gp91phox not accessible on intact neutrophils.
Specificity
This antibody recognises the heavy chain of cytochrome B245. It recognises the native as well as the denatured form of the protein
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CYBB
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Flow (Intracellular)
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Alternate Names for CYBB/NOX2 Antibody (NL7) [PE/Cy5.5]

  • CGD
  • CGD91-phox
  • CYBB
  • CYBB/NOX2
  • Cytochrome b(558) subunit beta
  • cytochrome b-245 heavy chain
  • cytochrome b-245, beta polypeptide
  • Cytochrome b558 subunit beta
  • EC 1.-.-.-
  • EC 1.6.3
  • gp91-1
  • GP91PHOX
  • gp91-phox
  • Heme-binding membrane glycoprotein gp91phox
  • NADPH oxidase 2
  • Neutrophil cytochrome b 91 kDa polypeptide
  • NOX2
  • NOX2chronic granulomatous disease
  • p22 phagocyte B-cytochrome
  • p91-PHOX
  • Superoxide-generating NADPH oxidase heavy chain subunit

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CYBB