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CYBB/NOX2 Antibody (NL7) [Allophycocyanin]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC
Clone
NL7
Clonality
Monoclonal
Host
Mouse
Conjugate
Allophycocyanin

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CYBB/NOX2 Antibody (NL7) [Allophycocyanin] Summary

Description
This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
Immunogen
Cytochrome B245 solubilized in octyl-beta-glucopyranoside
Epitope
Clone NL7 binds amino acids 498-506 (EKDVITGLK), a cytosolic epitope on gp91phox not accessible on intact neutrophils.
Specificity
This antibody recognises the heavy chain of cytochrome B245. It recognises the native as well as the denatured form of the protein
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CYBB
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Flow (Intracellular)
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Western Blot

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Alternate Names for CYBB/NOX2 Antibody (NL7) [Allophycocyanin]

  • CGD
  • CGD91-phox
  • CYBB
  • CYBB/NOX2
  • Cytochrome b(558) subunit beta
  • cytochrome b-245 heavy chain
  • cytochrome b-245, beta polypeptide
  • Cytochrome b558 subunit beta
  • EC 1.-.-.-
  • EC 1.6.3
  • gp91-1
  • GP91PHOX
  • gp91-phox
  • Heme-binding membrane glycoprotein gp91phox
  • NADPH oxidase 2
  • Neutrophil cytochrome b 91 kDa polypeptide
  • NOX2
  • NOX2chronic granulomatous disease
  • p22 phagocyte B-cytochrome
  • p91-PHOX
  • Superoxide-generating NADPH oxidase heavy chain subunit

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CYBB/NOX2 Antibody (NBP1-41012APC). (Showing 1 - 1 of 1 FAQ).

  1. Can this antibody be used on cells that have already been fixed?
    • Our product NBP1-41012APC can be used on fixed cells. This is our recommended protocol for ICC and for Flow (intracellular protocol)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CYBB
Entrez
Uniprot