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CXCL12/SDF-1 Antibody (79018) [Unconjugated]

Images

 
CXCL12/SDF-1 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs; positive staining) and K562 human chronic myelogenous leukemia cell line (negative staining) using Mouse Anti-Human/Mouse ...read more
CXCL12/SDF‑1 was detected in immersion fixed paraffin-embedded sections of human tonsil using 15 µg/mL Human/Mouse CXCL12/SDF‑1 Monoclonal Antibody (Catalog # MAB350) overnight at 4 °C. Tissue ...read more
CXCL12/SDF‑1 was detected in immersion fixed paraffin-embedded sections of human tonsil using Human/Mouse CXCL12/SDF‑1 Monoclonal Antibody (Catalog # MAB350) at 25 µg/mL overnight at 4 °C. Tissue ...read more
HT1080 human fibrosarcoma cell line was stained with Mouse Anti-Human/Mouse CXCL12/SDF-1 Monoclonal Antibody (Catalog # MAB350, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed ...read more
Orthogonal Strategies: CXCL12/SDF-1a mRNA was detected in formalin-fixed paraffin-embedded tissue sections of human tonsil probed with ACD RNAScope®Probe (Catalog # 422991) and stained using ACD ...read more
CXCR4 and CXCL12 expression and BM infiltration. (a) Expression analysis of CXCR4 and CXCL12 in uninfiltrated and infiltrated bone marrow specimens at the time of diagnosis by RQ-PCR. (b) Expression analysis of CXCR4 ...read more
RhoA modulates the CXCR4-CXCL12 axis in breast tumors. (a) Representative immunohistochemical (IHC) staining images of breast primary tumor sections of mice orthotopically injected with 4T1 GFP-LUC cells carrying ...read more
CXCR4 and CXCL12 expression in DLBCL. (a) Expression analysis of CXCR4 and CXCL12 in non-neoplastic control germinal center B cells (GC-B) and diffuse large B cell lymphoma cells (DLBCL) consisting of DLBCL-NGCB and ...read more
Tumor-promoting phenotype of the myeloid clusters.IHC staining demonstrating the expression of CD163, IL-6, IL-10, VEGF-A, MMP-9, SDF-1 and Bcl-xL by the myeloid cells associated with anthracosis. For each protein, ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications IHC, CyTOF-ready, Dual ISH-IHC, ELISA(Cap), Flow, ICC/IF
Clone
79018
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

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CXCL12/SDF-1 Antibody (79018) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human CXCL12/SDF‑1 alpha
Lys22-Lys89
Accession # Q6ICW0
Specificity
Detects human and mouse CXCL12/SDF-1 alpha and CXCL12/SDF-1 beta in ELISAs.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CXCL12
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Dual RNAscope ISH-IHC 5-25 ug/mL
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • ELISA Standard (Matched Pair)
  • ELISA Standard (Matched Pair)
  • Immunocytochemistry 8-15 ug/mL
  • Immunohistochemistry 8-25 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
Application Notes
ELISA Detection: Human/Mouse CXCL12/SDF-1 Biotinylated Antibody (Catalog number BAF310)
Standard: Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF-1alpha (Catalog number 350-NS)
Standard: Recombinant Mouse CXCL12/SDF-1alpha (Catalog number 460-SD) Use in ICC/IF reported in scientific literature (PMID:33435128)
Reviewed Applications
Read 1 Review rated 4
using
MAB350 in the following applications:

Publications
Read Publications using
MAB350 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCL12/SDF-1 Antibody (79018) [Unconjugated]

  • chemokine (C-X-C motif) ligand 12
  • C-X-C motif chemokine 12
  • CXCL12
  • hIRH
  • hSDF-1
  • Intercrine reduced in hepatomas
  • IRH
  • PBSF
  • PBSFSDF-1a
  • Pre-B cell growth-stimulating factor
  • SCYB12
  • SDF1
  • SDF-1
  • SDF1A
  • SDF1B
  • SDF1SDF-1b
  • stromal cell-derived factor 1
  • TLSF
  • TLSF-a
  • TLSF-b
  • TPAR1

Background

CXCL12, also known as SDF-1, is a CXC chemokine that interacts with CXCR4. SDF-1 alpha and SDF-1 beta are alternatively spliced isoforms of CXCL12. SDF-1 beta has an additional 4 amino acids at the carboxy-terminus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CXCL12/SDF-1 Antibody (MAB350)(149)

We have publications tested in 7 confirmed species: Human, Mouse, Rat, Feline, Rabbit, Transgenic Mouse, Xenograft.

We have publications tested in 17 applications: ELISA Capture, ELISA Development, ELISA Development (Capture), ELISA Development (Detection), Flow Cytometry, Functional Assay, ICC, IHC, IHC-Fr, IHC-P, Immunocytochemistry, Immunohistochemistry, In Vivo, Luminex Development, Mass Spectrometry, Neutralization, Western Blot.


Filter By Application
ELISA Capture
(2)
ELISA Development
(6)
ELISA Development (Capture)
(4)
ELISA Development (Detection)
(1)
Flow Cytometry
(6)
Functional Assay
(2)
ICC
(12)
IHC
(28)
IHC-Fr
(2)
IHC-P
(31)
Immunocytochemistry
(1)
Immunohistochemistry
(4)
In Vivo
(1)
Luminex Development
(3)
Mass Spectrometry
(1)
Neutralization
(3)
Western Blot
(2)
All Applications
Filter By Species
Human
(59)
Mouse
(32)
Rat
(4)
Feline
(2)
Rabbit
(2)
Transgenic Mouse
(2)
Xenograft
(1)
All Species
Showing Publications 1 - 10 of 149. Show All 149 Publications.
Publications using MAB350 Applications Species
Donnelly, H;Ross, E;Xiao, Y;Hermantara, R;Taqi, AF;Doherty-Boyd, WS;Cassels, J;Tsimbouri, PM;Dunn, KM;Hay, J;Cheng, A;Meek, RMD;Jain, N;West, C;Wheadon, H;Michie, AM;Peault, B;West, AG;Salmeron-Sanchez, M;Dalby, MJ; Bioengineered niches that recreate physiological extracellular matrix organisation to support long-term haematopoietic stem cells Nature communications 2024-07-10 [PMID: 38987295] (Immunocytochemistry, Human) Immunocytochemistry Human
Li, A;Yi, J;Li, X;Dong, L;Ostrow, LW;Ma, J;Zhou, J; Distinct transcriptomic profile of satellite cells contributes to preservation of neuromuscular junctions in extraocular muscles of ALS mice eLife 2024-04-25 [PMID: 38661532] (Immunohistochemistry, Mouse, Transgenic Mouse) Immunohistochemistry Mouse, Transgenic Mouse
Frenz-Wiessner, S;Fairley, SD;Buser, M;Goek, I;Salewskij, K;Jonsson, G;Illig, D;Zu Putlitz, B;Petersheim, D;Li, Y;Chen, PH;Kalauz, M;Conca, R;Sterr, M;Geuder, J;Mizoguchi, Y;Megens, RTA;Linder, MI;Kotlarz, D;Rudelius, M;Penninger, JM;Marr, C;Klein, C; Generation of complex bone marrow organoids from human induced pluripotent stem cells Nature methods 2024-02-19 [PMID: 38374263] (Immunohistochemistry, Human) Immunohistochemistry Human
Zheng, M;Kim, MH;Park, SG;Kim, WS;Oh, SH;Sung, JH; CXCL12 Neutralizing Antibody Promotes Hair Growth in Androgenic Alopecia and Alopecia Areata International journal of molecular sciences 2024-01-30 [PMID: 38338982] (Immunohistochemistry, Mouse) Immunohistochemistry Mouse
Pu, T;Wang, J;Wei, J;Zeng, A;Zhang, J;Chen, J;Yin, L;Li, J;Lin, TP;Melamed, J;Corey, E;Gao, AC;Wu, BJ; Stromal-derived MAOB promotes prostate cancer growth and progression Science advances 2024-02-09 [PMID: 38335292] (Immunohistochemistry, Human, Xenograft) Immunohistochemistry Human, Xenograft
Nguyen, JN;Mohan, EC;Pandya, G;Ali, U;Tan, C;Kofler, JK;Shapiro, L;Marrelli, SP;Chauhan, A; CD13 facilitates immune cell migration and aggravates acute injury but promotes chronic post-stroke recovery Journal of neuroinflammation 2023-10-10 [PMID: 37817190] (IHC, Human) IHC Human
Williamson, MR;Le, SP;Franzen, RL;Donlan, NA;Rosow, JL;Nicot-Cartsonis, MS;Cervantes, A;Deneen, B;Dunn, AK;Jones, TA;Drew, MR; Subventricular zone cytogenesis provides trophic support for neural repair in a mouse model of stroke Nature communications 2023-10-10 [PMID: 37816732] (IHC, Transgenic Mouse) IHC Transgenic Mouse
Chen S, Xing Z, Geng M et al. Macrophage fusion event as one prerequisite for inorganic nanoparticle-induced antitumor response Science advances 7/21/2023 [PMID: 37467339]
Ayano KUDO, Hiroki SAWAHATA, Sho YOSHIMOTO, Akinori YAMAUCHI, Ryo OSHITA, Eiichi KANAI et al. Evaluation of the influence of the C-X-C motif chemokine ligand 12 / C-X-C chemokine receptor 4 axis on canine mammary gland tumor cell migration Journal of Veterinary Medical Science 8/1/2023 [PMID: 37302847]
PK Suvas, M Setia, M Rana, A Chakrabort, S Suvas Novel characterization of CXCR4 expressing cells in uninfected and herpes simplex virus-1 infected corneas The ocular surface, 2023-02-20;28(0):99-107. 2023-02-20 [PMID: 36813133] (IHC, Mouse) IHC Mouse
Show All 149 Publications.

Review for CXCL12/SDF-1 Antibody (MAB350) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using MAB350:
Filter by Applications
IHC-Fr
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen CXCL12/SDF-1 MAB350
Enlarge
4
reviewed by:
Kirsten Hattermann-Koch
IHC-Fr Mouse 04/18/2019
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedAdult brain
SpeciesMouse
LotCOJ0518011

FAQs for CXCL12/SDF-1 Antibody (MAB350) (0)

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Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Kirsten Hattermann-Koch
04/18/2019
Application: IHC-Fr
Species: Mouse

Bioinformatics

Gene Symbol CXCL12
Uniprot