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Recombinant Human CXCL12/SDF-1 alpha Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE, Bioactivity
Format
Carrier-Free
Concentration
LYOPH

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Recombinant Human CXCL12/SDF-1 alpha Protein Summary

Description
A single non-glycosylated polypeptide chain containing 68 amino acids corresponding to CXCL12/SDF-1 alpha Source: E. coli

Uniprot ID: P48061

Amino Acid Sequence: KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKNNNRQVC IDPKLKWIQE YLEKALNK

Details of Functionality
SDF1 alpha Protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using PHA and rHuIL-2 activated human peripheral blood T-lymphocytes is in a concentration range of 20-80 ng/ml.
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
CXCL12
Purity
> 97 % pure by SDS-PAGE and HPLC
Endotoxin Note
Less than 1 EU/ug of CXCL12/SDF-1 alpha as determined by LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
  • SDS-Page
Theoretical MW
8.0 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB pH 7.0, 130 mM NaCl.
Preservative
No Preservative
Concentration
LYOPH
Purity
> 97 % pure by SDS-PAGE and HPLC
Reconstitution Instructions
Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.

Notes

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Alternate Names for Recombinant Human CXCL12/SDF-1 alpha Protein

  • CXCL12 chemokine (C-X-C motif) ligand 12
  • CXCL12/SDF-1 alpha
  • IRH
  • PBSF
  • SCYB12
  • SDF1 alpha
  • SDF-1 alpha
  • SDF-1 alpha/CXCL12 alpha
  • SDF1
  • SDF1a
  • SDF1B
  • TLSF
  • TPAR1

Background

CXCL12 also known as SDF-1 is belonging to the CXC chemokine family. It is encoded by the CXCL12 gene. In recently study, Human CXCL12 is expressed as six isoforms that differ only in the C-terminal tail. And all SDF-1 isoforms undergo proteolytic processing of the first two N-terminal amino acids. Contrast to the canonical sequence SDF-1 beta, SDF-1 alpha is shorter by four amino acids at the C-terminal tail. On the cell surface, the receptor for this chemokine is CXCR4 and syndecan4. CXCL12 is strongly chemotactic for T-lymphocytes, monocytes, but not neutrophils. CXCL12 is a very important factor in carcinogenesis and the neovascularisation linked to tumor progression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol CXCL12
Uniprot