Recombinant Human CLTB His Protein

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SDS-Page: CLTB Recombinant Protein [NBP1-98874]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
1 mg/ml

Order Details

Recombinant Human CLTB His Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-211 of Human CLTB

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMADDFGF FSSSESGAPE AAEEDPAAAF LAQQESEIAG IENDEGFGAP AGSHAAPAQP GPTSGAGSED MGTTVNGDVF QEANGPADGY AAIAQADRLT QEPESIRKWR EEQRKRLQEL DAASKVTEQE WREKAKKDLE EWNQRQSEQV EKNKINNRAS EEAFVKESKE ETPGTEWEKV AQLCDFNPKS SKQCKDVSRL RSVLMSLKQT PLSR

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
CLTB
Purity
>85%, by SDS-PAGE

Applications/Dilutions

Dilutions
  • SDS-Page
Theoretical MW
25.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl buffer (pH8.0), 10% glycerol, 0.2 mM PMSF, 100 mM NaCl
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>85%, by SDS-PAGE

Alternate Names for Recombinant Human CLTB His Protein

  • clathrin light chain B
  • clathrin, light chain (Lcb)
  • clathrin, light chain B
  • clathrin, light polypeptide (Lcb)
  • Lcb

Background

CLTB (Clathrin light chain B) belongs to the clathrin light chain family. Clathrin is a protein that plays a major role in the formation of coated vesicles. It forms a triskelion shape composed of three clathrin heavy chains and three light chains. When the triskelia interact they form a polyhedral lattice that surrounds the vesicle. Coat-proteins, like clathrin, are used to build small vesicles in order to safely transport molecules between cells. Clathrin-mediated endocytosis (CME) regulates many cellular physiological processes such as the internalization of growth factors and receptors, entry of pathogens, and synaptic transmission. Recombinant human CLTB protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol CLTB