![Western Blot: Clathrin Heavy Chain 1/CHC17 Antibody (X22) [NB300-613] - Analysis of 25 ug of human brain lysates.](http://images.novusbio.com/fullsize/Clathrin-Heavy-Chain-1-CHC17-Antibody-X22-Western-Blot-NB300-613-img0014.jpg "Western Blot: Clathrin Heavy Chain 1/CHC17 Antibody (X22) [NB300-613] - Analysis of 25 ug of human brain lysates.")
| Reactivity | Hu, Mu, Rt, Bv, Ha, PmSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, IP |
| Clone | X22 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 6 mg/ml |
| Immunogen | Purified human brain clathrin heavy chain. |
| Epitope | Electron microscopy and proteolysis mapping demonstrate that binding occurs towards the central hub of the triskelion, N-terminal to the light chain binding regions. |
| Localization | Cytoplasmic |
| Marker | Clathrin-coated Vesicle Marker |
| Specificity | Detects clathrin heavy chain. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CLTC |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
|
| Application Notes | WB: Detects a single approx. 180 kDa protein representing the clathrin heavy chain from bovine brain extract. IP: Triskelions with this antibody allows both the clathrin heavy chain and the associated light chain polypeptides to be examined. |
|
| Reviewed Applications |
|
|
| Publications |
|
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.05% Sodium Azide |
| Concentration | 6 mg/ml |
| Purity | Protein A purified |
![Simple Western alpha-Fetoprotein/AFP Antibody (189502) [Unconjugated]](https://images.novusbio.com/images2/16253.jpg)
![Knockout Validated alpha-Fetoprotein/AFP Antibody (189502) [Unconjugated]](https://images.novusbio.com/images2/alphaFetoprotein_MAB1368_Knockout_Validated_21503.jpg)
![Knockout Validated alpha-Fetoprotein/AFP Antibody (189502) [Unconjugated]](https://images.novusbio.com/images2/alphaFetoprotein_MAB1368_Knockout_Validated_22402.jpg)
![Simple Western Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Simple_Western_21888.jpg)
![Immunocytochemistry Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Immunocytochemistry__Immunofluorescence_17777.jpg)
![Immunohistochemistry Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Immunohistochemistry_23317.jpg)
| Images | Ratings | Applications | Species | Date | Details | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
reviewed by:
Verified Customer |
IF | Rat | 05/11/2010 |
Summary
|
Secondary Antibodies |
Isotype Controls |
Research Areas for Clathrin Heavy Chain 1/CHC17 Antibody (NB300-613)Find related products by research area.
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunocytochemistry/Immunofluorescence: Clathrin Heavy Chain 1/CHC17 Antibody (X22) [NB300-613] - Analysis of Clathrin, Heavy chain using Anti-Clathrin, Heavy chain Monoclonal Antibody (X22) shows staining in Hela Cells. Clathrin, Heavy chain staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Clathrin, Heavy chain at a dilution of 1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.](http://images.novusbio.com/fullsize/Clathrin-Heavy-Chain-1-CHC17-Antibody-X22-Immunocytochemistry-Immunofluorescence-NB300-613-img0012.jpg "Immunocytochemistry/Immunofluorescence: Clathrin Heavy Chain 1/CHC17 Antibody (X22) [NB300-613] - Analysis of Clathrin, Heavy chain using Anti-Clathrin, Heavy chain Monoclonal Antibody (X22) shows staining in Hela Cells. Clathrin, Heavy chain staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Clathrin, Heavy chain at a dilution of 1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.")
![Immunohistochemistry-Paraffin: Clathrin Heavy Chain 1/CHC17 Antibody (X22) [NB300-613] - Normal biopsies of deparaffinized Human colon tissue.](http://images.novusbio.com/fullsize/Clathrin-Heavy-Chain-1-CHC17-Antibody-X22-Immunohistochemistry-Paraffin-NB300-613-img0013.jpg "Immunohistochemistry-Paraffin: Clathrin Heavy Chain 1/CHC17 Antibody (X22) [NB300-613] - Normal biopsies of deparaffinized Human colon tissue.")
![Flow Cytometry: Clathrin Heavy Chain 1/CHC17 Antibody (X22) [NB300-613] - Analysis of Alexa Fluor (R) 488 conjugate of NB300-613. A cell surface stain was performed on HeLa cells with Clathrin Heavy Chain 1 antibody NB300-613AF488 (blue) along with a matched isotype control NBP2-27287AF488 (orange). Cells were incubated in an a](http://images.novusbio.com/fullsize/Clathrin-Heavy-Chain-1-CHC17-Antibody-X22-Flow-Cytometry-NB300-613-img0015.jpg "Flow Cytometry: Clathrin Heavy Chain 1/CHC17 Antibody (X22) [NB300-613] - Analysis of Alexa Fluor (R) 488 conjugate of NB300-613. A cell surface stain was performed on HeLa cells with Clathrin Heavy Chain 1 antibody NB300-613AF488 (blue) along with a matched isotype control NBP2-27287AF488 (orange). Cells were incubated in an a")
![Immunocytochemistry/Immunofluorescence: Clathrin Heavy Chain 1/CHC17 Antibody (X22) [NB300-613] - Analysis of Clathrin, Heavy chain using Anti-Clathrin, Heavy chain Monoclonal Antibody (X22) shows staining in U251 Cells. Clathrin, Heavy chain staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Clathrin, Heavy chain at a dilution of 1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.](http://images.novusbio.com/fullsize/Clathrin-Heavy-Chain-1-CHC17-Antibody-X22-Immunocytochemistry-Immunofluorescence-NB300-613-img0010.jpg "Immunocytochemistry/Immunofluorescence: Clathrin Heavy Chain 1/CHC17 Antibody (X22) [NB300-613] - Analysis of Clathrin, Heavy chain using Anti-Clathrin, Heavy chain Monoclonal Antibody (X22) shows staining in U251 Cells. Clathrin, Heavy chain staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Clathrin, Heavy chain at a dilution of 1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.")
![Immunocytochemistry/Immunofluorescence: Clathrin Heavy Chain 1/CHC17 Antibody (X22) [NB300-613] - Analysis of Clathrin, Heavy chain using Anti-Clathrin, Heavy chain Monoclonal Antibody (X22) shows staining in NCI-H460 Cells. Clathrin, Heavy chain staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Clathrin, Heavy chain at a dilution of 1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.](http://images.novusbio.com/fullsize/Clathrin-Heavy-Chain-1-CHC17-Antibody-X22-Immunocytochemistry-Immunofluorescence-NB300-613-img0011.jpg "Immunocytochemistry/Immunofluorescence: Clathrin Heavy Chain 1/CHC17 Antibody (X22) [NB300-613] - Analysis of Clathrin, Heavy chain using Anti-Clathrin, Heavy chain Monoclonal Antibody (X22) shows staining in NCI-H460 Cells. Clathrin, Heavy chain staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Clathrin, Heavy chain at a dilution of 1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.")
![Immunohistochemistry-Paraffin: Clathrin Heavy Chain 1/CHC17 Antibody (X22) [NB300-613] - Cancer biopsies of deparaffinized Human breast carcinoma tissue.](http://images.novusbio.com/fullsize/Clathrin-Heavy-Chain-1-CHC17-Antibody-X22-Immunohistochemistry-Paraffin-NB300-613-img0007.jpg "Immunohistochemistry-Paraffin: Clathrin Heavy Chain 1/CHC17 Antibody (X22) [NB300-613] - Cancer biopsies of deparaffinized Human breast carcinoma tissue.")
![SDS-PAGE TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNF-alpha_210-TA_256.jpg)
![Bioactivity TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNFalpha_210TA_1658.jpg)
![SEC-MALS TNF-alpha [Unconjugated]](https://images.novusbio.com/images/210-ta_recombinant-human-tnf-alpha-protein-sec-mals-35202312244..jpg)
![Immunocytochemistry Insulin Antibody (182410) [Unconjugated]](https://images.novusbio.com/images2/Insulin_MAB1417_Immunocytochemistry_9376.jpg)
![Immunohistochemistry Insulin Antibody (182410) [Unconjugated]](https://images.novusbio.com/images2/mab1417_human-bovine-mouse-insulin-mab-clone-182410-immunohistochemistry-308202115145.jpg)
![N/A Complement Component C5a [Biotin]](https://images.novusbio.com/images2/DATA_Complement_Component_C5a_DY2037_ELISA_1753.jpg)
![N/A IL-10 [Biotin]](https://images.novusbio.com/images2/DATA_IL10_DY417_ELISA_2014.jpg)
![Bioactivity IL-4 [Unconjugated]](https://images.novusbio.com/images/protein/6507-ilcf_recombinant-human-il-4-cho-expressed-protein-cf-bioactivity-272020133214.jpg)
![Bioactivity IFN-gamma [Unconjugated]](https://images.novusbio.com/images2/IFNgamma_285IF_2848.jpg)
![SDS-PAGE IFN-gamma [Unconjugated]](https://images.novusbio.com/images2/IFN-gamma_285-IF_230.jpg)
![SEC-MALS IFN-gamma [Unconjugated]](https://images.novusbio.com/images/285-if_recombinant-human-ifn-gamma-protein-sec-mals-1520239228..jpg)
![Western Blot ALK/CD246 Antibody [Unconjugated]](https://images.novusbio.com/images2/ALK_AF4210_Western_Blot_12746.jpg)
![Immunohistochemistry ALK/CD246 Antibody [Unconjugated]](https://images.novusbio.com/images2/12780.jpg)
![Simple Western ALK/CD246 Antibody [Unconjugated]](https://images.novusbio.com/images/antibody/af4210_human-alk-cd246-affinity-purified-pab-simple-western-296202315837.jpg)
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")