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Clathrin Heavy Chain 1/CHC17 Antibody (X22) [mFluor Violet 610 SE]

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Clathrin Heavy Chain 1/CHC17 Antibody (X22) [mFluor Violet 610 SE] [NB300-613MFV610] - Vial of mFluor Violet 610 conjugated antibody. mFluor Violet 610 is optimally excited at 421 nm by the Violet laser (405 nm) and has ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ha, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IP
Clone
X22
Clonality
Monoclonal
Host
Mouse
Conjugate
mFluor Violet 610 SE

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Clathrin Heavy Chain 1/CHC17 Antibody (X22) [mFluor Violet 610 SE] Summary

Immunogen
Purified human brain clathrin heavy chain.
Epitope
Electron microscopy and proteolysis mapping demonstrate that binding occurs towards the central hub of the triskelion, N-terminal to the light chain binding regions.
Localization
Cytoplasmic
Marker
Clathrin-coated Vesicle Marker
Specificity
Detects clathrin heavy chain.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CLTC
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunomicroscopy
  • Immunoprecipitation
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Hamster reactivity reported in scientific literature (PMID: 15689492). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Alternate Names for Clathrin Heavy Chain 1/CHC17 Antibody (X22) [mFluor Violet 610 SE]

  • CHC
  • CHC17
  • Clathrin Heavy Chain 1
  • Clathrin heavy chain on chromosome 17
  • clathrin, heavy chain (Hc)
  • clathrin, heavy chain
  • clathrin, heavy polypeptide (Hc)
  • clathrin, heavy polypeptide-like 2
  • CLH17
  • CLH-17
  • CLTC
  • CLTCL2
  • Hc
  • KIAA0034

Background

Clathrin is a major protein component of the cytoplasmic face of intracellular organelles, called coated vesicles and coated pits. These specialized organelles are involved in the intracellular trafficking of receptors and endocytosis of a variety of macromolecules. The basic subunit of the clathrin coat is composed of three heavy chains (192 kDa) and three light chains (LCa or LCb, 25-29 kDa) and self assembles into a polyhedral cytoskeletal element. Self assembly facilitates clathrin's central role in membrane sorting and selective transport within the cell.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Clathrin Heavy Chain 1/CHC17 Antibody (NB300-613MFV610) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CLTC