GLI-3 Antibody

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Human GLI-3 is a 190 kDa transcription factor of the C2H2-type zinc-finger protein family. GLI-3 appears to play a role in limb and brain development, probably through sonic hedgehog signaling. GLI-3 is regulated by PKA-mediated phosphorylation. Unphosphorylated full-length GLI-3 is a transcriptional activator, while phosphorylated GLI-3 is cleaved after amino acid 700 to generate an N-terminal 83 kDa transcriptional repressor. Over the region used as immunogen, human GLI-3 is 95% identical to the corresponding mouse and canine protein sequences.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 4 confirmed species: Human, Mouse, C. elegans, Transgenic Mouse.

We have publications tested in 8 applications: Bioassay, CUT&Tag, ChIP, ICC, IHC, IHC-P, Immunoprecipitation, Western Blot.

Showing Publications 1 - 10 of 102. Show All 102 Publications. Collapse Publications.

Publications using AF3690	Applications	Species
U?pie?ski, T;Niewiadomski, P; The Proteasome and Cul3-Dependent Protein Ubiquitination Is Required for Gli Protein-Mediated Activation of Gene Expression in the Hedgehog Pathway Cells 2024-09-06 [PMID: 39273066] (Western Blot, Mouse)	Western Blot	Mouse
Baran, B;Derua, R;Janssens, V;Niewiadomski, P; PP2A phosphatase regulatory subunit PPP2R3C is a new positive regulator of the hedgehog signaling pathway Cellular signalling 2024-08-20 [PMID: 39173855] (Western Blot, Human)	Western Blot	Human
Zhonghao Deng, Shengwei Rong, Lu Gan, Fuhua Wang, Liangxiao Bao, Fang Cai et al. Temporal transcriptome features identify early skeletal commitment during human epiphysis development at single-cell resolution iScience 8/18/2023 [PMID: 37554462]
Meropi Bagka, Hyeonyi Choi, Margaux Héritier, Hanna Schwaemmle, Quentin T. L. Pasquer, Simon M. G. Braun et al. Targeted protein degradation reveals BET bromodomains as the cellular target of Hedgehog pathway inhibitor-1 Nature Communications 2023-07-01 [PMID: 37393376]
P Horák, K Kreisinger, J Réda, L Ondrušová, J Balko, J Vachtenhei, P Žáková, J Vachtenhei The Hedgehog/GLI signaling pathway activates transcription of Slug (Snail2) in melanoma cells Oncology reports, 2023-03-03;49(4):. 3/3/2023 [PMID: 36866769]
JS Fleck, SMJ Jansen, D Wollny, F Zenk, M Seimiya, A Jain, R Okamoto, M Santel, Z He, JG Camp, B Treutlein Inferring and perturbing cell fate regulomes in human brain organoids Nature, 2022-10-05;0(0):. 2022-10-05 [PMID: 36198796] (Bioassay, CUT&Tag, IHC, Human)	Bioassay, CUT&Tag, IHC	Human
M Kurtovi?, N Piteša, N Bartoni?ek, P Ozreti?, V Musani, J ?onkaš, T Petri?, C King, M Sabol RNA-seq and ChIP-seq Identification of Unique and Overlapping Targets of GLI Transcription Factors in Melanoma Cell Lines Cancers, 2022-09-19;14(18):. 2022-09-19 [PMID: 36139698] (ChIP, Human)	ChIP	Human
Megan Rowton, Carlos Perez-Cervantes, Suzy Hur, Jessica Jacobs-Li, Emery Lu, Nikita Deng et al. Hedgehog signaling activates a mammalian heterochronic gene regulatory network controlling differentiation timing across lineages Developmental Cell 2022-09-14 [PMID: 36108627]
Jianjian Zhu, Rashmi Patel, Anna Trofka, Brian D. Harfe, Susan Mackem Sonic hedgehog is not a limb morphogen but acts as a trigger to specify all digits in mice Developmental Cell 2022-09-01 [PMID: 35977544]
S Schmidt, MD Luecken, D Trümbach, S Hembach, KM Niedermeie, N Wenck, K Pflügler, C Stautner, A Böttcher, H Lickert, C Ramirez-Su, R Ahmad, MJ Ziller, JC Fitzgerald, V Ruf, WDJ van de Ber, AJ Jonker, T Gasser, B Winner, J Winkler, DM Vogt Weise, F Giesert, FJ Theis, W Wurst Primary cilia and SHH signaling impairments in human and mouse models of Parkinson's disease Nature Communications, 2022-08-16;13(1):4819. 2022-08-16 [PMID: 35974013] (IHC, Human)	IHC	Human
Leah Schembs, Ariane Willems, Kerstin Hasenpusch-Theil, James D. Cooper, Katie Whiting, Karen Burr et al. The ciliary gene INPP5E confers dorsal telencephalic identity to human cortical organoids by negatively regulating Sonic hedgehog signaling Cell Reports 2022-05-17 [PMID: 35584663]
Juuri E, Tikka P, Domanskyi A et al. Ptch2 is a Potential Regulator of Mesenchymal Stem Cells Frontiers in Physiology 2022-04-28 [PMID: 35574464]
Skylar I. Dewees, Romana Vargová, Katherine R. Hardin, Rachel E. Turn, Saroja Devi, Joshua Linnert et al. Phylogenetic profiling and cellular analyses of ARL16 reveal roles in traffic of IFT140 and INPP5E Molecular Biology of the Cell 2022-04-01 [PMID: 35196065]
J Sun, J Yoon, M Lee, HK Lee, YS Hwang, IO Daar Zic5 stabilizes Gli3 via a non-transcriptional mechanism during retinal development Cell Reports, 2022-02-01;38(5):110312. 2022-02-01 [PMID: 35108539] (Immunoprecipitation, Western Blot, Human)	Immunoprecipitation, Western Blot	Human
D Sharma, AJ Mirando, A Leinroth, JT Long, CM Karner, MJ Hilton HES1 is a novel downstream modifier of the SHH-GLI3 Axis in the development of preaxial polydactyly PloS Genetics, 2021-12-20;17(12):e1009982. 2021-12-20 [PMID: 34928956] (Western Blot, Mouse, Transgenic Mouse)	Western Blot	Mouse, Transgenic Mouse
Rachel E. Turn, Yihan Hu, Skylar I. Dewees, Narra Devi, Michael P. East, Katherine R. Hardin et al. The ARF GAPs ELMOD1 and ELMOD3 act at the Golgi and cilia to regulate ciliogenesis and ciliary protein traffic Molecular Biology of the Cell 2022-02-01 [PMID: 34818063]
Anna Trofka, Bau-Lin Huang, Jianjian Zhu, William F. Heinz, Valentin Magidson, Yuki Shibata et al. Genetic basis for an evolutionary shift from ancestral preaxial to postaxial limb polarity in non-urodele vertebrates Current Biology 2021-11-01 [PMID: 34610275]
Karen E. Boschen, Eric W. Fish, Scott E. Parnell Prenatal alcohol exposure disrupts Sonic hedgehog pathway and primary cilia genes in the mouse neural tube Reproductive Toxicology 2021-10-01 [PMID: 34492310]
Yingyi Z, Jielu H, Mariana T et al. FBF1 deficiency promotes beiging and healthy expansion of white adipose tissue. Cell Rep. 2021-08-03 [PMID: 34348145]
Sun-Hee Hwang, Bandarigoda N Somatilaka, Kevin White, Saikat Mukhopadhyay Ciliary and extraciliary Gpr161 pools repress hedgehog signaling in a tissue-specific manner eLife 2021-08-04 [PMID: 34346313]
Ching-Fang Chang, Kari M. Brown, Yanfen Yang, Samantha A. Brugmann Centriolar Protein C2cd3 Is Required for Craniofacial Development Frontiers in Cell and Developmental Biology 2021-06-15 [PMID: 34211969]
Chuan Chen, Qingwen Xu, Yuxia Zhang, Brian A. Davies, Yan Huang, David J. Katzmann et al. Ciliopathy protein HYLS1 coordinates the biogenesis and signaling of primary cilia by activating the ciliary lipid kinase PIPKI gamma Science Advances 2021-06-25 [PMID: 34162535]
Truong ME, Bilekova S, Choksi SP et al. Vertebrate cells differentially interpret ciliary and extraciliary cAMP Cell 4/27/2021 [PMID: 33932338]
A Koeniger, A Brichkina, I Nee, L Dempwolff, A Hupfer, I Galperin, F Finkernage, A Nist, T Stiewe, T Adhikary, W Diederich, M Lauth Activation of Cilia-Independent Hedgehog/GLI1 Signaling as a Novel Concept for Neuroblastoma Therapy Cancers, 2021-04-15;13(8):. 2021-04-15 [PMID: 33921042] (Western Blot, Mouse)	Western Blot	Mouse
Elena A. May, Marian Kalocsay, Inès Galtier D’Auriac, Patrick S. Schuster, Steven P. Gygi, Maxence V. Nachury et al. Time-resolved proteomics profiling of the ciliary Hedgehog response Journal of Cell Biology 2021-05-03 [PMID: 33856408]
Rachel E. Turn, Joshua Linnert, Eduardo D. Gigante, Uwe Wolfrum, Tamara Caspary, Richard A. Kahn Roles for ELMOD2 and Rootletin in ciliogenesis Molecular Biology of the Cell 2021-04-15 [PMID: 33596093]
Virginie Mournetas, Emmanuelle Massouridès, Jean‐Baptiste Dupont, Etienne Kornobis, Hélène Polvèche, Margot Jarrige et al. Myogenesis modelled by human pluripotent stem cells: a multi‐omic study of Duchenne myopathy early onset Journal of Cachexia, Sarcopenia and Muscle 2021-02-14 [PMID: 33586340]
KH Elliott, X Chen, J Salomone, P Chaturvedi, PA Schultz, SK Balchand, JD Servetas, A Zuniga, R Zeller, B Gebelein, MT Weirauch, KA Peterson, SA Brugmann Gli3 utilizes Hand2 to synergistically regulate tissue-specific transcriptional networks Elife, 2020-10-02;9(0):. 2020-10-02 [PMID: 33006313] (Western Blot, Mouse)	Western Blot	Mouse
JM Dias, Z Alekseenko, A Jeggari, M Boareto, J Vollmer, M Kozhevniko, H Wang, MP Matise, A Alexeyenko, D Iber, J Ericson A Shh/Gli-driven three-node timer motif controls temporal identity and fate of neural stem cells Science Advances, 2020-09-16;6(38):. 2020-09-16 [PMID: 32938678] (ICC, Immunoprecipitation, Western Blot, Mouse)	ICC, Immunoprecipitation, Western Blot	Mouse
Kerstin Hasenpusch-Theil, Christine Laclef, Matt Colligan, Eamon Fitzgerald, Katherine Howe, Emily Carroll et al. A transient role of the ciliary gene Inpp5e in controlling direct versus indirect neurogenesis in cortical development eLife 2020-08-25 [PMID: 32840212]
Saishu Yoshida, Katsuhiko Aoki, Ken Fujiwara, Takashi Nakakura, Akira Kawamura, Kohji Yamada et al. The novel ciliogenesis regulator DYRK2 governs Hedgehog signaling during mouse embryogenesis eLife 2020-08-06 [PMID: 32758357]
Fabian Freisleben, Lena Behrmann, Vanessa Thaden, Jana Muschhammer, Carsten Bokemeyer, Walter Fiedler et al. Downregulation of GLI3 Expression Mediates Chemotherapy Resistance in Acute Myeloid Leukemia International Journal of Molecular Sciences 2020-07-18 [PMID: 32708452]
Bandarigoda Nipunika Somatilaka, Sun-Hee Hwang, Vivek Reddy Palicharla, Kevin Andrew White, Hemant Badgandi, John Michael Shelton et al. Ankmy2 Prevents Smoothened-Independent Hyperactivation of the Hedgehog Pathway via Cilia-Regulated Adenylyl Cyclase Signaling Developmental Cell 2020-09-01 [PMID: 32702291]
RU Chottekala, S Kalik, J Gresack, A Ayala, M Gao, W Wang, S Meller, A Aly, A Schaefer, P Greengard AP-1 controls the p11-dependent antidepressant response Mol. Psychiatry, 2020-05-21;0(0):. 2020-05-21 [PMID: 32439846] (IHC, C. elegans)	IHC	C. elegans
H Yan, C Chen, H Chen, H Hong, Y Huang, K Ling, J Hu, Q Wei TALPID3 and ANKRD26 selectively orchestrate FBF1 localization and cilia gating Nat Commun, 2020-05-04;11(1):2196. 2020-05-04 [PMID: 32366837] (IHC, C. elegans)	IHC	C. elegans
Y Kim, J Lee, M Seppala, MT Cobourne, SH Kim Ptch2/Gas1 and Ptch1/Boc differentially regulate Hedgehog signalling in murine primordial germ cell migration Nat Commun, 2020-04-24;11(1):1994. 2020-04-24 [PMID: 32332736] (IHC, Mouse)	IHC	Mouse
Marisa E. Hom, Alison E. Ondrus, Tomoyo Sakata‐Kato, Paul G. Rack, James K. Chen Bicyclic Imidazolium Inhibitors of Gli Transcription Factor Activity ChemMedChem 2020-06-17 [PMID: 32268014]
Katherine Q Chen, Naoyuki Tahara, Aaron Anderson, Hiroko Kawakami, Sho Kawakami, Ryuichi Nishinakamura et al. Development of the Proximal-Anterior Skeletal Elements in the Mouse Hindlimb Is Regulated by a Transcriptional and Signaling Network Controlled by Sall4 Genetics 2020-05-01 [PMID: 32156750]
Eduardo D Gigante, Megan R Taylor, Anna A Ivanova, Richard A Kahn, Tamara Caspary ARL13B regulates Sonic hedgehog signaling from outside primary cilia eLife 2020-03-04 [PMID: 32129762]
P Liu, M Dodson, D Fang, E Chapman, DD Zhang NRF2 negatively regulates primary ciliogenesis and hedgehog signaling PLoS Biol., 2020-02-13;18(2):e3000620. 2020-02-13 [PMID: 32053600] (Western Blot, Mouse)	Western Blot	Mouse
Sandii Constable, Alyssa B. Long, Katharine A. Floyd, Stéphane Schurmans, Tamara Caspary The ciliary phosphatidylinositol phosphatase Inpp5e plays positive and negative regulatory roles in Shh signaling Development 2020-01-01 [PMID: 31964774]
MF Bastida, R Pérez-Góme, A Trofka, J Zhu, A Rada-Igles, R Sheth, HS Stadler, S Mackem, MA Ros The formation of the thumb requires direct modulation of Gli3 transcription by Hoxa13 Proc. Natl. Acad. Sci. U.S.A., 2020-01-02;0(0):. 2020-01-02 [PMID: 31896583] (Western Blot, Mouse)	Western Blot	Mouse
EZM Taroc, A Naik, JM Lin, NB Peterson, DL Keefe, E Genis, G Fuchs, R Balasubram, PE Forni Gli3 regulates vomeronasal neurogenesis, olfactory ensheathing cell formation and GnRH-1 neuronal migration J. Neurosci., 2019-11-25;0(0):. 2019-11-25 [PMID: 31767679] (IHC, Mouse)	IHC	Mouse
A Martin-Hur, R Martin-Mor, N Robledinos, R Blanco, I Palacios-B, I Lastres-Be, A Cuadrado, FR Garcia-Gon NRF2-dependent gene expression promotes ciliogenesis and Hedgehog signaling Sci Rep, 2019-09-25;9(1):13896. 2019-09-25 [PMID: 31554934] (ICC, Western Blot, Mouse)	ICC, Western Blot	Mouse
Francesca Bufalieri, Paola Infante, Flavia Bernardi, Miriam Caimano, Paolo Romania, Marta Moretti et al. ERAP1 promotes Hedgehog-dependent tumorigenesis by controlling USP47-mediated degradation of beta TrCP Nature Communications 2019-07-24 [PMID: 31341163]
Heejin Nam, Shin Jeon, Hyejin An, Jaeyoung Yoo, Hyo-Jong Lee, Soo-Kyung Lee et al. Critical roles of ARHGAP36 as a signal transduction mediator of Shh pathway in lateral motor columnar specification eLife 2019-07-15 [PMID: 31305241]
P Ma, NN Song, Y Li, Q Zhang, L Zhang, L Zhang, Q Kong, L Ma, X Yang, B Ren, C Li, X Zhao, Y Li, Y Xu, X Gao, YQ Ding, B Mao Fine-Tuning of Shh/Gli Signaling Gradient by Non-proteolytic Ubiquitination during Neural Patterning Cell Rep, 2019-07-09;28(2):541-553.e4. 2019-07-09 [PMID: 31291587] (Western Blot, Human, Mouse)	Western Blot	Human, Mouse
Yuhong Han, Bing Wang, Yong Suk Cho, Jian Zhu, Jiang Wu, Yongbin Chen et al. Phosphorylation of Ci/Gli by Fused Family Kinases Promotes Hedgehog Signaling Developmental Cell 2019-09-01 [PMID: 31279575]
Christina R Adams, Htet Htwe Htwe, Timothy Marsh, Aprilgate L Wang, Megan L Montoya, Lakshmipriya Subbaraj et al. Transcriptional control of subtype switching ensures adaptation and growth of pancreatic cancer eLife 2019-05-28 [PMID: 31134896]
Anna A. Kiseleva, Vladislav A. Korobeynikov, Anna S. Nikonova, Peishan Zhang, Petr Makhov, Alexander Y. Deneka et al. Unexpected Activities in Regulating Ciliation Contribute to Off-target Effects of Targeted Drugs Clinical Cancer Research 2019-07-01 [PMID: 30867219]
K He, X Ma, T Xu, Y Li, A Hodge, Q Zhang, J Torline, Y Huang, J Zhao, K Ling, J Hu Axoneme polyglutamylation regulated by Joubert syndrome protein ARL13B controls ciliary targeting of signaling molecules Nat Commun, 2018-08-17;9(1):3310. 2018-08-17 [PMID: 30120249] (ICC, Human)	ICC	Human
Kerstin Hasenpusch-Theil, Stephen West, Alexandra Kelman, Zrinko Kozic, Sophie Horrocks, Andrew P. McMahon et al. Gli3 controls the onset of cortical neurogenesis by regulating the radial glial cell cycle through Cdk6 expression Development 2018-08-20 [PMID: 30093555]
SR Hong, CL Wang, YS Huang, YC Chang, YC Chang, GV Pusapati, CY Lin, N Hsu, HC Cheng, YC Chiang, WE Huang, NC Shaner, R Rohatgi, T Inoue, YC Lin Spatiotemporal manipulation of ciliary glutamylation reveals its roles in intraciliary trafficking and Hedgehog signaling Nat Commun, 2018-04-30;9(1):1732. 2018-04-30 [PMID: 29712905] (ICC, Mouse)	ICC	Mouse
P Infante, R Faedda, F Bernardi, F Bufalieri, L Lospinoso, R Alfonsi, D Mazzà, M Siler, S Coni, A Po, M Petroni, E Ferretti, M Mori, E De Smaele, G Canettieri, C Capalbo, M Maroder, I Screpanti, M Kool, SM Pfister, D Guardavacc, A Gulino, L Di Marcotu Itch/?-arrestin2-dependent non-proteolytic ubiquitylation of SuFu controls Hedgehog signalling and medulloblastoma tumorigenesis Nat Commun, 2018-03-07;9(1):976. 3/7/2018 [PMID: 29515120]
T Rosengren, LJ Larsen, LB Pedersen, ST Christense, LB Møller TSC1 and TSC2 regulate cilia length and canonical Hedgehog signaling via different mechanisms Cell. Mol. Life Sci., 2018-02-02;0(0):. 2018-02-02 [PMID: 29396625] (Western Blot, Mouse)	Western Blot	Mouse
A Struchtrup, A Wiegering, B Stork, U Rüther, C Gerhardt The ciliary protein RPGRIP1L governs autophagy independently of its proteasome-regulating function at the ciliary base in mouse embryonic fibroblasts Autophagy, 2018-02-21;0(0):1-47. 2018-02-21 [PMID: 29372668] (Western Blot, Mouse)	Western Blot	Mouse
LK Veistinen, T Mustonen, MR Hasan, M Takatalo, Y Kobayashi, DA Kesper, A Vortkamp, DP Rice Regulation of Calvarial Osteogenesis by Concomitant De-repression of GLI3 and Activation of IHH Targets Front Physiol, 2017-12-19;8(0):1036. 2017-12-19 [PMID: 29311969] (IHC, Western Blot, Mouse)	IHC, Western Blot	Mouse
Ganesh V. Pusapati, Jennifer H. Kong, Bhaven B. Patel, Arunkumar Krishnan, Andreas Sagner, Maia Kinnebrew et al. CRISPR Screens Uncover Genes that Regulate Target Cell Sensitivity to the Morphogen Sonic Hedgehog Developmental Cell 2018-01-08 [PMID: 29290584]
Yeon‐Joo Kim, Daniel PS Osborn, Ji‐Young Lee, Masatake Araki, Kimi Araki, Timothy Mohun et al. WDR11‐mediated Hedgehog signalling defects underlie a new ciliopathy related to Kallmann syndrome EMBO reports 2018-02-01 [PMID: 29263200]
VJ Guen, TE Chavarria, C Kröger, X Ye, RA Weinberg, JA Lees EMT programs promote basal mammary stem cell and tumor-initiating cell stemness by inducing primary ciliogenesis and Hedgehog signaling Proc. Natl. Acad. Sci. U.S.A., 2017-11-20;0(0):. 2017-11-20 [PMID: 29158396] (IHC-P, Mouse)	IHC-P	Mouse
D Hasegawa, H Ochiai-Shi, S Onodera, T Nakamura, A Saito, T Onda, K Watanabe, K Nishimura, M Ohtaka, M Nakanishi, K Kosaki, A Yamaguchi, T Shibahara, T Azuma Gorlin syndrome-derived induced pluripotent stem cells are hypersensitive to hedgehog-mediated osteogenic induction PLoS ONE, 2017-10-31;12(10):e0186879. 2017-10-31 [PMID: 29088246] (Western Blot, Human)	Western Blot	Human
Naoki Nakagawa, Jingjun Li, Keiko Yabuno-Nakagawa, Tae-Yeon Eom, Martis Cowles, Tavien Mapp et al. APC sets the Wnt tone necessary for cerebral cortical progenitor development Genes & Development 2017-08-15 [PMID: 28916710]
J. Aaron Crapster, Louanne Hudgins, James K. Chen, Natalia Gomez-Ospina A novel missense variant in the GLI3 zinc finger domain in a family with digital anomalies American Journal of Medical Genetics Part A 2017-12-01 [PMID: 28884880]
Gonnissen A, Isebaert S, Perneel C et al. Tissue microarray analysis indicates hedgehog signaling as a potential prognostic factor in intermediate-risk prostate cancer BMC Cancer 9/6/2017 [PMID: 28877722]
J Li, Y Cui, J Xu, Q Wang, X Yang, Y Li, X Zhang, M Qiu, Z Zhang, Z Zhang Suppressor of Fused Restraint of Hedgehog Activity Level is Critical for Osteogenic Proliferation and Differentiation during Calvarial Bone Development J. Biol. Chem., 2017-08-09;0(0):. 2017-08-09 [PMID: 28794157] (Western Blot, Mouse)	Western Blot	Mouse
Y Han, Y Xiong, X Shi, J Wu, Y Zhao, J Jiang Regulation of Gli ciliary localization and Hedgehog signaling by the PY-NLS/karyopherin-?2 nuclear import system PLoS Biol., 2017-08-04;15(8):e2002063. 2017-08-04 [PMID: 28777795] (Western Blot, Mouse)	Western Blot	Mouse
Maria A. Hoelzl, Karin Heby-Henricson, Marco Gerling, José M. Dias, Raoul V. Kuiper, Cornelius Trünkle et al. Differential requirement of SUFU in tissue development discovered in a hypomorphic mouse model Developmental Biology 2017-09-01 [PMID: 28688895]
Nadia B. Hassounah, Martha Nunez, Colleen Fordyce, Denise Roe, Ray Nagle, Thomas Bunch et al. Inhibition of Ciliogenesis Promotes Hedgehog Signaling, Tumorigenesis, and Metastasis in Breast Cancer Molecular Cancer Research 2017-10-01 [PMID: 28611083]
AM Arensdorf, ME Dillard, JM Menke, MW Frank, CO Rock, SK Ogden Sonic Hedgehog Activates Phospholipase A2 to Enhance Smoothened Ciliary Translocation Cell Rep, 2017-06-06;19(10):2074-2087. 2017-06-06 [PMID: 28591579] (ICC, Western Blot, Mouse)	ICC, Western Blot	Mouse
H Cai, A Liu Spop regulates Gli3 activity and Shh signaling in dorsoventral patterning of the mouse spinal cord Dev. Biol., 2017-04-12;0(0):. 2017-04-12 [PMID: 28412462] (Western Blot, Mouse)	Western Blot	Mouse
Siew Cheng Phua, Shuhei Chiba, Masako Suzuki, Emily Su, Elle C Roberson, Ganesh V Pusapati et al. Dynamic Remodeling of Membrane Composition Drives Cell Cycle through Primary Cilia Excision Cell 2017-01-12 [PMID: 28086093]
Illegitimate translation causes unexpected gene expression from on-target out-of-frame alleles created by CRISPR-Cas9 Sci Rep, 2016-12-21;6(0):39608. 2016-12-21 [PMID: 28000783] (Western Blot, Mouse)	Western Blot	Mouse
Spop promotes skeletal development and homeostasis by positively regulating Ihh signaling Proc. Natl. Acad. Sci. U.S.A, 2016-12-05;0(0):. 2016-12-05 [PMID: 27930311] (Western Blot, Human)	Western Blot	Human
Craniofacial Ciliopathies Reveal Specific Requirements for GLI Proteins during Development of the Facial Midline PLoS Genet., 2016-11-01;12(11):e1006351. 2016-11-01 [PMID: 27802276] (Western Blot, Mouse)	Western Blot	Mouse
Ya-Ting Chang, Praneet Chaturvedi, Elizabeth N. Schock, Samantha A. Brugmann Understanding Mechanisms of GLI-Mediated Transcription during Craniofacial Development and Disease Using the Ciliopathic Mutant, talpid2 Frontiers in Physiology 2016-10-17 [PMID: 27799912]
Cholesterol activates the G-protein coupled receptor Smoothened to promote morphogenetic signaling Elife, 2016-10-05;5(0):. 2016-10-05 [PMID: 27705744] (Western Blot, Mouse)	Western Blot	Mouse
Mechanism of inhibition of the tumor suppressor Patched by Sonic Hedgehog Proc Natl Acad Sci USA, 2016-09-19;0(0):. 2016-09-19 [PMID: 27647915] (Western Blot, Mouse)	Western Blot	Mouse
Zhigang Jin, Tyler Schwend, Jia Fu, Zehua Bao, Jing Liang, Huimin Zhao et al. Members of the Rusc protein family interact with Sufu and inhibit vertebrate Hedgehog signaling Development 2016-04-22 [PMID: 27633991]
Shinichi Hayashi, Ryutaro Akiyama, Julia Wong, Naoyuki Tahara, Hiroko Kawakami, Yasuhiko Kawakami Gata6-Dependent GLI3 Repressor Function is Essential in Anterior Limb Progenitor Cells for Proper Limb Development PLOS Genetics 2016-06-28 [PMID: 27352137]
U Emechebe, P Kumar P, JM Rozenberg, B Moore, A Firment, T Mirshahi, AM Moon T-box3 is a ciliary protein and regulates stability of the Gli3 transcription factor to control digit number Elife, 2016-04-05;5(0):. 2016-04-05 [PMID: 27046536] (IHC-P, Mouse)	IHC-P	Mouse
Sharda Prasad Yadav, Neel Kamal Sharma, Chunqiao Liu, Lijin Dong, Tiansen Li, Anand Swaroop Centrosomal protein CP110 controls maturation of the mother centriole during cilia biogenesis Development 2016-05-01 [PMID: 26965371]
Sohinee Bhattacharyya, Mark A Rainey, Priyanka Arya, Bhopal C. Mohapatra, Insha Mushtaq, Samikshan Dutta et al. Endocytic recycling protein EHD1 regulates primary cilia morphogenesis and SHH signaling during neural tube development Scientific Reports 2016-02-17 [PMID: 26884322]
Gerhardt C, Lier JM, Burmuhl S et al. The transition zone protein Rpgrip1l regulates proteasomal activity at the primary cilium J. Cell Biol. 7/6/2015 [PMID: 26150391]
Taylor SP, Dantas TJ, Duran I et al. Mutations in DYNC2LI1 disrupt cilia function and cause short rib polydactyly syndrome. Nat Commun 6/16/2015 [PMID: 26077881]
Jianying Li, Qihui Wang, Ying Cui, Xueqin Yang, Yan Li, Xiaoyun Zhang et al. Suppressor of Fused Is Required for Determining Digit Number and Identity via Gli3/Fgfs/Gremlin PLOS ONE 2015-05-22 [PMID: 26001200]
Adriana Eisner, Maria F. Pazyra-Murphy, Ershela Durresi, Pengcheng Zhou, Xuesong Zhao, Emily C. Chadwick et al. The Eya1 Phosphatase Promotes Shh Signaling during Hindbrain Development and Oncogenesis Developmental Cell 2015-04-06 [PMID: 25816987]
Makino S, Zhulyn O, Mo R, Puviindran V, Zhang X, Murata T, Fukumura R, Ishitsuka Y, Kotaki H, Matsumaru D, Ishii S, Hui C, Gondo Y T396I mutation of mouse Sufu reduces the stability and activity of Gli3 repressor. PLoS ONE, 2015-03-11;10(3):e0119455. 2015-03-11 [PMID: 25760946] (Western Blot, Mouse)	Western Blot	Mouse
Chong Y, Mann R, Zhao C, Kato M, Beachy P Bifurcating action of Smoothened in Hedgehog signaling is mediated by Dlg5. Genes Dev, 2015-02-01;29(3):262-76. 2015-02-01 [PMID: 25644602] (Western Blot, Mouse)	Western Blot	Mouse
Zhulyn O, Hui C Sufu and Kif7 in limb patterning and development. Dev Dyn, 2015-01-24;244(3):468-78. 2015-01-24 [PMID: 25581370] (Western Blot, Mouse)	Western Blot	Mouse
Chih-Wei Fan, Baozhi Chen, Irene Franco, Jianming Lu, Heping Shi, Shuguang Wei et al. The Hedgehog Pathway Effector Smoothened Exhibits Signaling Competency in the Absence of Ciliary Accumulation Chemistry & Biology 2014-12-18 [PMID: 25484239]
Hynes , Ann Mari, Giles , Rachel H, Srivastava , Shalabh, Eley , Lorraine, Whitehead , Jennifer, Danilenko , Marina, Raman , Shreya, Slaats , Gisela G, Colville , John G, Ajzenberg , Henry, Kroes , Hester Y, Thelwall , Peter E, Simmons , Nicholas, Miles , Colin G, Sayer , John A Murine Joubert syndrome reveals Hedgehog signaling defects as a potential therapeutic target for nephronophthisis. Proc Natl Acad Sci U S A, 2014-06-19;111(27):9893-8. 2014-06-19 [PMID: 24946806] (Western Blot, Mouse)	Western Blot	Mouse
Zhulyn O, Li D, Deimling S, Vakili N, Mo R, Puviindran V, Chen M, Chuang P, Hopyan S, Hui C A switch from low to high Shh activity regulates establishment of limb progenitors and signaling centers. Dev Cell, 2014-04-10;29(2):241-9. 2014-04-10 [PMID: 24726283] (Western Blot, Mouse)	Western Blot	Mouse
Liu Y, Tsai I, Morleo M, Oh E, Leitch C, Massa F, Lee B, Parker D, Finley D, Zaghloul N, Franco B, Katsanis N Ciliopathy proteins regulate paracrine signaling by modulating proteasomal degradation of mediators. J Clin Invest, 2014-04-01;124(5):2059-70. 2014-04-01 [PMID: 24691443] (Western Blot, Mouse)	Western Blot	Mouse
Kozhemyakina , Elena, Ionescu , Andreia, Lassar , Andrew B GATA6 is a crucial regulator of Shh in the limb bud. PLoS Genet, 2014-01-09;10(1):e1004072. 2014-01-09 [PMID: 24415953] (Western Blot, Mouse)	Western Blot	Mouse
Donnelly J, Engevik A, Engevik M, Schumacher M, Xiao C, Yang L, Worrell R, Zavros Y Gastritis promotes an activated bone marrow-derived mesenchymal stem cell with a phenotype reminiscent of a cancer-promoting cell. Dig Dis Sci, 2013-11-08;59(3):569-82. 11/8/2013 [PMID: 24202649]
Nachtergaele S, Whalen DM, Mydock LK et al. Structure and function of the Smoothened extracellular domain in vertebrate Hedgehog signaling. Elife. 10/29/2013 [PMID: 24171105]
Renault M, Vandierdonck S, Chapouly C, Yu Y, Qin G, Metras A, Couffinhal T, Losordo D, Yao Q, Reynaud A, Jaspard-Vinassa B, Belloc I, Desgranges C, Gadeau A Gli3 regulation of myogenesis is necessary for ischemia-induced angiogenesis. Circ Res, 2013-09-17;113(10):1148-58. 2013-09-17 [PMID: 24044950] (Western Blot, Mouse)	Western Blot	Mouse
Deschaseaux F, Gaillard J, Langonne A, Chauveau C, Naji A, Bouacida A, Rosset P, Heymann D, De Pinieux G, Rouas-Freiss N, Sensebe L Regulation and function of immunosuppressive molecule human leukocyte antigen G5 in human bone tissue. FASEB J, 2013-04-16;27(8):2977-87. 2013-04-16 [PMID: 23592762] (Western Blot, Human)	Western Blot	Human
Christoph Gerhardt, Johanna M. Lier, Stefanie Kuschel, Ulrich Rüther The Ciliary Protein Ftm Is Required for Ventricular Wall and Septal Development PLoS ONE 2013-02-28 [PMID: 23469020]
Cigna N, Farrokhi Moshai E, Brayer S, Marchal-Somme J, Wemeau-Stervinou L, Fabre A, Mal H, Leseche G, Dehoux M, Soler P, Crestani B, Mailleux A The hedgehog system machinery controls transforming growth factor-beta-dependent myofibroblastic differentiation in humans: involvement in idiopathic pulmonary fibrosis. Am J Pathol, 2012-09-29;181(6):2126-37. 2012-09-29 [PMID: 23031257] (IHC-P, Human)	IHC-P	Human
Tanimoto Y, Veistinen L, Alakurtti K Prevention of premature fusion of calvarial suture in GLI-Kruppel family member 3 (Gli3)-deficient mice by removing one allele of Runt-related transcription factor 2 (Runx2). J. Biol. Chem., 2012-04-30;287(25):21429-38. 2012-04-30 [PMID: 22547067] (Western Blot, Mouse)	Western Blot	Mouse
Humke EW, Dorn KV, Milenkovic L, Scott MP, Rohatgi R The output of Hedgehog signaling is controlled by the dynamic association between Suppressor of Fused and the Gli proteins. Genes Dev., 2010-04-01;24(7):670-82. 2010-04-01 [PMID: 20360384] (Immunoprecipitation, Mouse)	Immunoprecipitation	Mouse
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Images	Ratings	Applications	Species	Date	Details
Enlarge	5 reviewed by: Verified Customer	WB	Mouse	11/13/2015	View Summary Application Western Blot Sample Tested NIH-3T3 cells Species Mouse Lot YLD0109011
	5 reviewed by: Verified Customer	WB	Mouse	01/08/2015	View Summary Application Western Blot Sample Tested See PMID 22173325 Species Mouse
	4 reviewed by: Verified Customer	WB	Mouse	01/08/2015	View Summary Application Western Blot Sample Tested See PMID 22547067 Species Mouse

Array AF3690

• GLI-3 Antibodies
• GLI-3 Proteins