I need a proven anti-human Ptch1 antibody that maps C-terminus of Ptch1 protein which is thus appropriate for all isoforms of human Ptch1 protein. Can you please suggest any which can be used for Western blotting?

Name: Patched 1/PTCH Antibody (413220) [Unconjugated] - (First Extracellular Loop)
Brand: Novus Biologicals
SKU: MAB41051
Price: 449 USD

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Accepted Answer

NB100-41101 is expected to recognize all isoforms. NBP2-19705, NBP1-71662, 21130002, and NBP1-59455 should all suit your needs as well. Further details about each product can be found on their individual datasheets.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	Hu, MuSpecies Glossary
Applications	WB, IHC
Clone	413220
Clonality	Monoclonal
Host	Rat
Conjugate	Unconjugated
Concentration	LYOPH

Catalog# & Formulation	Size	Price
Carrier Free MAB41051-SP

Immunogen	E. coli-derived recombinant mouse Patched 1/PTCH Glu108-Asp422 Accession # Q61115
Specificity	Detects mouse Patched 1/PTCH (First Extracellular Loop) in direct ELISAs and Western blots. In direct ELISAs and Western blots, 15% cross‑reactivity with recombinant human Patched 2 is observed.
Source	N/A
Isotype	IgG2a
Clonality	Monoclonal
Host	Rat
Gene	PTCH1
Purity Statement	Protein A or G purified from hybridoma culture supernatant
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative	No Preservative
Concentration	LYOPH
Reconstitution Instructions	Reconstitute at 0.5 mg/mL in sterile PBS.

Patched 1/PTCH Antibody (413220) [Unconjugated] - (First Extracellular Loop)

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Publications using MAB41051	Applications	Species
Suppiah, S;Mansouri, S;Mamatjan, Y;Liu, JC;Bhunia, MM;Patil, V;Rath, P;Mehani, B;Heir, P;Bunda, S;Velez-Reyes, GL;Singh, O;Ijad, N;Pirouzmand, N;Dalcourt, T;Meng, Y;Karimi, S;Wei, Q;Nassiri, F;Pugh, TJ;Bader, GD;Aldape, KD;Largaespada, DA;Zadeh, G; Multiplatform molecular profiling uncovers two subgroups of malignant peripheral nerve sheath tumors with distinct therapeutic vulnerabilities Nature communications 2023-05-10 [PMID: 37164978] (Western Blot, Human)	Western Blot	Human
Nicole Lustgarten Guahmich, Limor Man, Jerry Wang, Laury Arazi, Eleni Kallinos, Ariana Topper-Kroog et al. Human theca arises from ovarian stroma and is comprised of three discrete subtypes Communications Biology 2023-01-04 [PMID: 36599970]
ÁJ Feliz More, A Hasanovic, A Morin, C Prunier, V Magnone, K Lebrigand, A Aouad, S Cogoluegne, J Favier, C Pasquier, I Mus-Veteau Persistent Properties of a Subpopulation of Cancer Cells Overexpressing the Hedgehog Receptor Patched Pharmaceutics, 2022-05-05;14(5):. 2022-05-05 [PMID: 35631574] (Flow Cytometry, ICC, Western Blot, Human)	Flow Cytometry, ICC, Western Blot	Human
S Tanigawa, M Fujita, C Moyama, S Ando, H Ii, Y Kojima, T Fujishita, M Aoki, H Takeuchi, T Yamanaka, Y Takahashi, N Hashimoto, S Nakata Inhibition of Gli2 suppresses tumorigenicity in glioblastoma stem cells derived from a de novo murine brain cancer model Cancer Gene Therapy, 2021-01-07;0(0):. 2021-01-07 [PMID: 33414520] (Western Blot, Mouse)	Western Blot	Mouse
Tessier S, Madhu V, Johnson ZI et al. NFAT5/TonEBP controls early acquisition of notochord phenotypic markers, collagen composition, and sonic hedgehog signaling during mouse intervertebral disc embryogenesis Dev. Biol. 7/10/2019 [PMID: 31301300]
N Sharon, J Vanderhoof, J Straubhaar, J Mueller, R Chawla, Q Zhou, EN Engquist, C Trapnell, DK Gifford, DA Melton Wnt Signaling Separates the Progenitor and Endocrine Compartments during Pancreas Development Cell Rep, 2019-05-21;27(8):2281-2291.e5. 2019-05-21 [PMID: 31116975] (IHC, Mouse)	IHC	Mouse
Raffaella Bonavita, Kathleen Vincent, Robert Pinelli, Chitra Lekha Dahia Formation of the sacrum requires down-regulation of sonic hedgehog signaling in the sacral intervertebral discs Biology Open 2018-07-24 [PMID: 29784673]
Renata E. Gordon, Li Zhang, Suraj Peri, Yin-Ming Kuo, Fang Du, Brian L. Egleston et al. Statins Synergize with Hedgehog Pathway Inhibitors for Treatment of Medulloblastoma Clinical Cancer Research 2018-03-15 [PMID: 29437795]
Vitte J, Gao F, Coppola G et al. Timing of Smarcb1 and Nf2 inactivation determines schwannoma versus rhabdoid tumor development Nat Commun 8/21/2017 [PMID: 28824165]
Lancaster MA, Gopal DJ, Kim J Defective Wnt-dependent cerebellar midline fusion in a mouse model of Joubert syndrome. Nat. Med., 2011-05-29;17(6):726-31. 2011-05-29 [PMID: 21623382] (IHC-Fr, Mouse)	IHC-Fr	Mouse

Gene Symbol	PTCH1
Uniprot	Q61115()