GLI-1 Antibody - BSA Free

Gli1 is a transcription factor involved in development, patterning and the regulation of cell fate-determination. A down-stream effector of the Hedgehog (HH) signaling pathway, Gli proteins work with other transcriptional activators to up-regulate the expression of Gli-response genes. In addition to its role in embryogenesis, the aberrant activation of the HH/Gli pathway has been identified in numerous forms of cancer, implicating the Gli family of proteins as potential therapeutic targets. In the absence of a stimulus, Gli1 is anchored to the cytoplasm via Cos-2, which is then released and translocates to the nucleus upon activation of the HH pathway. In the nucleus, Gli1 binds DNA via a highly conserved C2-H2 zinc finger domain and acts exclusively as an activator increasing transcription of GLI1, PTCH1, PTCH2, HHIP1, MYCN, CCND1, CCND2, BCL2, CFLAR, FOXF1, FOXL1, PRDM1 (BLIMP1), JAG2, GREM1, and Follistatin.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 4 confirmed species: Human, Mouse, Canine, Porcine.

We have publications tested in 8 applications: ChIP, Chemotaxis, FLOW, ICC/IF, IF/IHC, IHC-P, Immunohistochemistry, WB.

Showing Publications 1 - 10 of 55. Show All 55 Publications. Collapse Publications.

Publications using NB600-600	Applications	Species
Orzechowska-Licari EJ, Bialkowska AB, Yang VW Sonic Hedgehog and WNT signaling regulate a positive feedback loop between intestinal epithelial and stromal cells to promote epithelial regeneration Cellular and molecular gastroenterology and hepatology 2023-07-20 [PMID: 37481204] (WB, Mouse) Details: 1:400 WB dilution	WB	Mouse
Vera RE, Lamberti MJ, Almada LL et al. GLI1 interaction with p300 modulates SDF1 expression in cancer-associated fibroblasts to promote pancreatic cancer cells migration The Biochemical journal 2023-02-15 [PMID: 36734208]
Dias RB. Estudo do perfil de expressao de componentes da via de sinalizacao sonic hedgehog em displasia epitelial oral [Study of the expression of components of sonic hedgehog signaling pathway in oral epithelial dysplasia profile. Thesis: http://arca.icict.fiocruz.br/handle/icict/7483]. Thesis (IHC-P, Human)	IHC-P	Human
Bo Ram Kim, Dae Yeong Kim, Na Ly Tran, Bu Gyeom Kim, Sun Il Lee, Sang Hee Kang, Byung Yook Min, Wooyoung Hur, Sang Cheul Oh Daunorubicin induces GLI1-dependent apoptosis in colorectal cancer cell lines International Journal of Oncology 2024-06-01 [PMID: 38757343]
Matko Leović, Antonija Jakovčević, Ivan Mumlek, Irena Zagorac, Maja Sabol, Dinko Leović, Marko Tarle A Pilot Immunohistochemical Study Identifies Hedgehog Pathway Expression in Sinonasal Adenocarcinoma International Journal of Molecular Sciences 2024-04-24 [PMID: 38731849]
Hong Zhou, Qun Zhang, Chenyang Liu, Jiahao Fan, Wen Huang, Nan Li, Mingxia Yang, Hong Wang, Weiping Xie, Hui Kong NLRP3 inflammasome mediates abnormal epithelial regeneration and distal lung remodeling in silica-induced lung fibrosis International Journal of Molecular Medicine 2024-03-01 [PMID: 38240085]
Koo H, Lee S, Kim WH Usability of serum hedgehog signalling proteins as biomarkers in canine mammary carcinomas BMC veterinary research 2023-11-06 [PMID: 37932728] (WB, Canine)	WB	Canine
Wang M, Huang W FOXS1 promotes prostate cancer progression through the Hedgehog/Gli1 pathway Biochemical pharmacology 2023-10-27 [PMID: 37890593]
Almada LL, Barroso K, Sen S et al. GLI1, a novel target of the ER stress regulator p97/VCP, promotes ATF6f-mediated activation of XBP1 Biochimica et biophysica acta. Gene regulatory mechanisms 2023-02-24 [PMID: 36842643] (ChIP)	ChIP
Liu Y, Liu S, Song Z et al. GLI1 Deficiency Impairs the Tendon-Bone Healing after Anterior Cruciate Ligament Reconstruction: In Vivo Study Using Gli1-Transgenic Mice Journal of Clinical Medicine 2023-01-28 [PMID: 36769647] (Immunohistochemistry)	Immunohistochemistry
Yamakawa D, Tsuboi J, Kasahara K et al. Cilia-Mediated Insulin/Akt and ST2/JNK Signaling Pathways Regulate the Recovery of Muscle Injury Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2022-11-14 [PMID: 36373718] (WB, Mouse) Details: Dilution used in WB 1:1000	WB	Mouse
Gonzalez-Rodriguez P, Cheray M, Keane L et al. ULK3-dependent activation of GLI1 promotes DNMT3A expression upon autophagy induction Autophagy 2022-02-28 [PMID: 35226587] (WB, ICC/IF, Human)	WB, ICC/IF	Human
Reyes NicolAs V, Allaire J, Alfonso A Et al. Altered Mucus Barrier Integrity and Increased Susceptibility to Colitis in Mice upon Loss of Telocyte Bone Morphogenetic Protein Signalling Cells 2021-10-29 [PMID: 34831177] (ICC/IF, Mouse)	ICC/IF	Mouse
Dalton GD, Oh SH, Tang L et al. Hepatocyte activity of the cholesterol sensor smoothened regulates cholesterol and bile acid homeostasis in mice iScience 2021-09-24 [PMID: 34568800] (WB, Mouse)	WB	Mouse
Olson AW, Le V, Wang J Et al. Stromal androgen and hedgehog signaling regulates stem cell niches in pubertal prostate development Development (Cambridge, England) 2021-08-24 [PMID: 34427305]
Shi Y, Liao X, Long JY Et al. Gli1+ progenitors mediate bone anabolic function of teriparatide via Hh and Igf signaling Cell reports 2021-08-17 [PMID: 34407400] (Mouse)		Mouse
Ichikawa D, Yamashita K, Okuno Y et al. Integrated diagnosis based on transcriptome analysis in suspected pediatric sarcomas NPJ genomic medicine 2021-06-15 [PMID: 34131151] (IHC-P, Human)	IHC-P	Human
Wei Y, Sun H, Gui T et al. The critical role of Hedgehog-responsive mesenchymal progenitors in meniscus development and injury repair eLife 2021-06-04 [PMID: 34085927] (IF/IHC, Porcine, Mouse, Human)	IF/IHC	Porcine, Mouse, Human
Boukhalfa A, Roccio F, Dupont N et al. The autophagy protein ATG16L1 cooperates with IFT20 and INPP5E to regulate the turnover of phosphoinositides at the primary cilium Cell reports 2021-04-27 [PMID: 33910006]
Wang W, Yan T, Guo W et al. Constitutive GLI1 expression in chondrosarcoma is regulated by major vault protein via mTOR/S6K1 signaling cascade Cell death and differentiation 2021-02-26 [PMID: 33637972] (Chemotaxis)	Chemotaxis
Tanigawa S, Fujita M, Moyama C, et al. Inhibition of Gli2 suppresses tumorigenicity in glioblastoma stem cells derived from a de novo murine brain cancer model Cancer gene therapy 2021-01-07 [PMID: 33414520]
Qian Wang, Shengnan Jia, Ding Wang, Xuyang Chen, Dhan V Kalvakolanu, Hongwu Zheng, Xiaodong Wei, Naiyan Wen, Hang Liang, Baofeng Guo, Ling Zhang A Combination of BRD4 and HDAC3 Inhibitors Synergistically Suppresses Glioma Stem Cell Growth by Blocking GLI1/IL6/STAT3 Signaling Axis. Molecular cancer therapeutics 2021-08-12 [PMID: 32999044]
Singh SK, Gordetsky JB, Bae S et al. Selective Targeting of the Hedgehog Signaling Pathway by PBM Nanoparticles in Docetaxel-Resistant Prostate Cancer Cells 2020-08-27 [PMID: 32867229] (FLOW, IF/IHC, ICC/IF, Human)	FLOW, IF/IHC, ICC/IF	Human
Bacelar Sacramento de AraUjo T, de Oliveira Siquara da Rocha L, Torres Andion Vidal M et al. GANT61 Reduces Hedgehog Molecule (GLI1) Expression and Promotes Apoptosis in Metastatic Oral Squamous Cell Carcinoma Cells International journal of molecular sciences 2020-08-24 [PMID: 32846867] (WB, ICC/IF, Human)	WB, ICC/IF	Human
Evelyn Susanto, Ana Marin Navarro, Leilei Zhou, Anders Sundström, Niek van Bree, Marina Stantic, Mohsen Moslem, Jignesh Tailor, Jonne Rietdijk, Veronica Zubillaga, Jens-Martin Hübner, Holger Weishaupt, Johanna Wolfsberger, Irina Alafuzoff, Ann Nordgren, Thierry Magnaldo, Peter Siesjö, John Inge Johnsen, Marcel Kool, Kristiina Tammimies, Anna Darabi, Fredrik J. Swartling, Anna Falk, Margareta Wilhelm Modeling SHH-driven medulloblastoma with patient iPS cell-derived neural stem cells Proceedings of the National Academy of Sciences of the United States of America 2020-08-18 [PMID: 32747535]
Lin YL, Lin YW, Nhieu J et al. Sonic Hedgehog-Gli1 Signaling and Cellular Retinoic Acid Binding Protein 1 Gene Regulation in Motor Neuron Differentiation and Diseases Int J Mol Sci 2020-06-09 [PMID: 32527063] (Chemotaxis, Mouse)	Chemotaxis	Mouse
Safgren SL, Olson RLO, Vrabel AM et al. The transcription factor GLI1 cooperates with the chromatin remodeler SMARCA2 to regulate chromatin accessibility at distal DNA regulatory elements J. Biol. Chem. 2020-05-06 [PMID: 32376693]
Oak ASW, Bocheva G, Kim TK et al. Noncalcemic Vitamin D Hydroxyderivatives Inhibit Human Oral Squamous Cell Carcinoma and Down-regulate Hedgehog and WNT/beta-Catenin Pathways Anticancer Res. 2020-05-01 [PMID: 32366390] (ICC/IF, Human)	ICC/IF	Human
Chen PY, Qin L, Li G et al. Smooth Muscle Cell Reprogramming in Aortic Aneurysms Cell Stem Cell 2020-04-02 [PMID: 32243809] (Mouse)		Mouse
Petty AJ, Li A, Wang X et al. Hedgehog signaling promotes tumor-associated macrophage polarization to suppress intratumoral CD8+ T cell recruitment J. Clin. Invest. 2019-10-22 [PMID: 31638600] (Chemotaxis, Mouse)	Chemotaxis	Mouse
Mazzone A, Gibbons SJ, Eisenman ST et al. Direct repression of anoctamin 1 ( ANO1) gene transcription by Gli proteins FASEB J. 2019-02-25 [PMID: 30802137] (Chemotaxis, Human)	Chemotaxis	Human
Wang K, Chen D, Qian Z et al. Hedgehog/Gli1 signaling pathway regulates MGMT expression and chemoresistance to temozolomide in human glioblastoma. Cancer Cell Int. 2017-12-04 [PMID: 29225516] (Chemotaxis, Human)	Chemotaxis	Human
Tanno B, Leonardi S, Babini G et al. Nanog-driven cell-reprogramming and self-renewal maintenance in Ptch1 +/- granule cell precursors after radiation injury Sci Rep. 2017-10-27 [PMID: 29079783] (WB, Mouse)	WB	Mouse
Wei R, Lv M, Li F et al. Human CAFs promote lymphangiogenesis in ovarian cancer via the Hh-VEGF-C signaling axis. Oncotarget. 2017-09-15 [PMID: 28978035] (IHC-P, Mouse)	IHC-P	Mouse
Schneider RK, Mullally A, Dugourd A et al. Gli1(+) Mesenchymal Stromal Cells Are a Key Driver of Bone Marrow Fibrosis and an Important Cellular Therapeutic Target. Cell Stem Cell. 2017-04-13 [PMID: 28457748] (Mouse)		Mouse
Xu T, Zhang H, Park SS et al. Loss of Pin1 Suppresses Hedgehog-Driven Medulloblastoma Tumorigenesis. Neoplasia. 2017-02-03 [PMID: 28167297]
Puri P Bushnell D Schaefer CM, Bates CM. Six2creFrs2a knockout mice are a novel model of renal cystogenesis. Sci Rep. 2016-11-17 [PMID: 27853247] (WB, Mouse)	WB	Mouse
Wu M, Ingram L, Tolosa EJ et al. Gli transcription factors mediate the oncogenic transformation of prostate basal cells induced by a Kras-androgen receptor axis. J. Biol. Chem. 2016-10-19 [PMID: 27760825] (Chemotaxis, Mouse)	Chemotaxis	Mouse
Vidal MT, Lourenco SV, Soares FA et al. The sonic hedgehog signaling pathway contributes to the development of salivary gland neoplasms regardless of perineural infiltration. Tumour Biol. 2016-01-21 [PMID: 26790448] (IHC-P, Human)	IHC-P	Human
Valverde LF, Pereira TA, Dias RB et al. Macrophages and endothelial cells orchestrate tumor-associated angiogenesis in oral cancer via hedgehog pathway activation. Tumour Biol. 2016-01-15 [PMID: 26768620] (IHC-P, Human)	IHC-P	Human
Comba A, Almada LL, Tolosa EJ et al. Nuclear factor of activated T cells-dependent downregulation of the transcription factor GLI1 underlies the growth inhibitory properties of arachidonic acid. J. Biol. Chem. 2015-11-24 [PMID: 26601952] (Chemotaxis, Human)	Chemotaxis	Human
Yeddula N, Xia Y, Ke E et al. Screening for tumor suppressors: Loss of ephrin receptor A2 cooperates with oncogenic KRas in promoting lung adenocarcinoma. Proc. Natl. Acad. Sci. U.S.A. 2015-11-24 [PMID: 26542681] (IHC-P, Mouse)	IHC-P	Mouse
Jackson DA, Smith TD, Amarsaikhan N et al. Modulation of the IL-6 Receptor alpha Underlies GLI2-Mediated Regulation of Ig Secretion in Waldenstrom Macroglobulinemia Cells. J Immunol 2015-09-15 [PMID: 26238488]
Jiang H, Li F, He C et al. Expression of Gli1 and Wnt2B correlates with progression and clinical outcome of pancreatic cancer. Int J Clin Exp Pathol. 2014-08-14 [PMID: 25120849] (IHC-P, Human)	IHC-P	Human
Nye MD, Almada LL, Fernandez-Barrena MG et al. The transcription factor GLI1 interacts with SMAD proteins to modulate TGFbeta-induced gene expression in a PCAF-dependent manner. J. Biol. Chem. 2014-04-16 [PMID: 24739390] (Chemotaxis, WB, Human)	Chemotaxis, WB	Human
Huang L, Walter V, Hayes DN. Hedgehog-GLI Signaling Inhibition Suppresses Tumor Growth in Squamous Lung Cancer. Clin. Cancer Res. 2014-03-05 [PMID: 24423612] (WB, Human)	WB	Human
Mazumdar T, Sandhu R, Qadan M et al. Hedgehog Signaling Regulates Telomerase Reverse Transcriptase in Human Cancer Cells. PLoS One. 2013-09-25 [PMID: 24086482] (Chemotaxis, WB, Human)	Chemotaxis, WB	Human
Regard JB, Malhotra D, Gvozdenovic-Jeremic J et al. Activation of Hedgehog signaling by loss of GNAS causes heterotopic ossification. Nat Med. 2013-09-29 [PMID: 24076664] (WB, Mouse)	WB	Mouse
Karlou M, Lu JF, Wu G et al. Hedgehog signaling inhibition by the small molecule smoothened inhibitor GDC-0449 in the bone forming prostate cancer xenograft MDA PCa 118b Prostate 2012-11-01 [PMID: 22457212] (IF/IHC, Human)	IF/IHC	Human
Mazumdar T, Devecchio J, Agyeman A et al. Blocking Hedgehog survival signaling at the level of the GLI genes induces DNA damage and extensive cell death in human colon carcinoma cells. Cancer Res. 2011-07-11 [PMID: 21747117]
Mazumdar T, DeVecchio J, Shi T et al. Hedgehog signaling drives cellular survival in human colon carcinoma cells Cancer Res 2011-02-01 [PMID: 21135115] (WB, Human)	WB	Human
Dos Santos JN. Estudo imunoistoquimico de componentes da via Sonic Hedgehog, STAT3 e MCM3 em tumores de glandula salivar. Thesis. 2015-01-01 (IHC-P, Human)	IHC-P	Human
Tzelepi V, Karlou M, Wen S et al. Expression of hedgehog pathway components in prostate carcinoma microenvironment: shifting the balance towards autocrine signalling. Histopathology;58(7):1037-1047. 2011-06-01 [PMID: 21707705] (IF/IHC, Human)	IF/IHC	Human
Breunig JJ, Sarkisian MR, Arellano JI et al. Primary cilia regulate hippocampal neurogenesis by mediating sonic hedgehog signaling. PNAS;105(35):13127-13132. 2008-01-01 [PMID: 15170664]
Niemann C et al. Indian hedgehog beta-catenin signaling: role in the sebaceous lineage of normal neoplastic mammalian epidermis. Proc. Natl. Acad. Sci. USA 100 Suppl 1:11873-80. 2003-01-01 [PMID: 12917489] (Chemotaxis, WB, Human)	Chemotaxis, WB	Human
Show All 55 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
	5 reviewed by: Verified Customer	IHC-P	Canine	06/12/2019	View Summary Application Immunohistochemistry-Paraffin Sample Tested Skin (hair follicle) Species Canine Lot C-2 Comments Comments Antibody dilution = 1:600. 3, 3'-diaminobenzidine (DAB) chromogen and counterstaining with Mayer’s hematoxylin.

Array NB600-600

• GLI-1 Antibodies
• GLI-1 ELISA Kits
• GLI-1 Lysate
• GLI-1 Proteins