Novus Biologicals products are now on bio-techne.com

Suppressor of Fused Antibody

Images

 
Western Blot: Suppressor of Fused Antibody [NBP1-87384] - Analysis in human cell line HEL.
Immunocytochemistry/ Immunofluorescence: Suppressor of Fused Antibody [NBP1-87384] - Staining of human cell line U-251 MG shows localization to nucleus. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Suppressor of Fused Antibody [NBP1-87384] - Staining of human Tonsil shows moderate nuclear positivity in germinal and non-germinal center cells.
Immunohistochemistry-Paraffin: Suppressor of Fused Antibody [NBP1-87384] - Staining of human testis shows high expression.
Immunohistochemistry-Paraffin: Suppressor of Fused Antibody [NBP1-87384] - Staining of human Cerebral cortex shows strong nuclear positivity in neuronal and glial cells.
Immunohistochemistry-Paraffin: Suppressor of Fused Antibody [NBP1-87384] - Staining of human Kidney shows strong nuclear positivity in cells in tubules.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Suppressor of Fused Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: TEELHSAQQWNGQGILELLRTVPIAGGPWLITDMRRGETIFEIDPHLQERVDKGIETDGSNLSGVSAKCAWDDLSRPPEDDEDSRSICIGTQPRRLSGKDTEQIRETLRRGLEINSKPVLPPINPQRQNGLAHDRAPSRKDSLESDSSTA
Predicted Species
Mouse (97%), Rat (95%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SUFU
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Suppressor of Fused Protein (NBP1-87384PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Suppressor of Fused Antibody

  • SUFUHSUFUXLPRO1280
  • suppressor of fused homolog (Drosophila)
  • suppressor of fused homolog

Background

The Hedgehog (Hh) signaling pathway has conserved roles in development of species ranging from Drosophila to humans. Human supressor of fused HSu(Fu) acts as a negative regulator in the hedgehog signaling (SHH) pathway by down-regulating GLI1-mediated transactivation of target genes (1). HSu(fu) was found to repress activity of the zinc-finger transcription factor Gli, which mediates Hedgehog signaling in vertebrates, and to physically interact with Gli, Gli2 and Gli3 (2). The sonic hedgehog signaling pathway directs the embryonic development of diverse organisms and is disrupted in a variety of malignancies. Several of mutations encode truncated proteins that are unable to export the GLI transcription factor from nucleus to cytoplasm, resulting in the activation of SHH signaling. SUFU is a newly identified tumor-suppressor gene that predisposes individuals to medulloblastoma by modulating the SHH signaling pathway through a newly identified mechanism (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB600-600
Species: Ca, Hu, Mu
Applications: ChIP, ELISA, Flow-IC, IB, ICC/IF, IHC, IHC-P, KD, WB
NBP3-35576
Species: Hu, Mu, Rt
Applications: ELISA, WB
H00003077-M01
Species: Hu
Applications: ELISA, ICC/IF, WB
NLS2666
Species: Bt, Bv, Ca, Eq, Ha, Hu, Pm, Mu, Po, Rb, Rt
Applications: ICC/IF, IHC, IHC-P
MAB41051
Species: Hu, Mu
Applications: IHC, WB
NBP3-04509
Species: Hu, Mu, Rt
Applications: ELISA, IHC, IHC-P, WB
NBP1-92172
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
AF3635
Species: Mu
Applications: IHC, WB
NBP1-83411
Species: Hu
Applications: ICC/IF, IHC, IHC-P
AF3690
Species: Hu, Mu
Applications: ChIP, ICC, WB
AF1705
Species: Mu
Applications: IHC, WB
AF1568
Species: Mu
Applications: WB
NBP1-33581
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P
AF482
Species: Mu
Applications: IHC, WB
NBP1-85351
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB

Publications for Suppressor of Fused Antibody (NBP1-87384) (0)

There are no publications for Suppressor of Fused Antibody (NBP1-87384).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Suppressor of Fused Antibody (NBP1-87384) (0)

There are no reviews for Suppressor of Fused Antibody (NBP1-87384). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Suppressor of Fused Antibody (NBP1-87384) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Suppressor of Fused Products

Research Areas for Suppressor of Fused Antibody (NBP1-87384)

Find related products by research area.

Blogs on Suppressor of Fused

There are no specific blogs for Suppressor of Fused, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Suppressor of Fused Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol SUFU