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Human CD133 ELISA Kit (Colorimetric)

Name: Human CD133 ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP3-38977

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ELISA: Human CD133 ELISA Kit (Colorimetric) [NBP3-38977] - Standard Curve Reference

Product Details

Summary

Reactivity	HuSpecies Glossary
Applications	ELISA
Suitable Sample Type	Tissue homogenates, cell lysates and other biological fluids
Standard Curve Range	0.156 - 10 ng/mL (example only; lot dependent)
Sensitivity	0.064 ng/mL (example only; lot dependent)

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Human CD133 ELISA Kit (Colorimetric) Summary

Description	Assay Length: 4.5 hours
Standard Curve Range	0.156 - 10 ng/mL (example only; lot dependent)
Sensitivity	0.064 ng/mL (example only; lot dependent)
Assay Type	Sandwich ELISA
Inter-Assay	%CV < 12 (example only; lot dependent)
Intra-Assay	%CV < 10 (example only; lot dependent)
Sample Volume	100 uL
Kit Type	ELISA Kit (Colorimetric)
Gene	PROM1

Applications/Dilutions

Dilutions

ELISA

Packaging, Storage & Formulations

Storage

Storage of components varies. See protocol for specific instructions.

Kit Components

Components

Detection Reagent A
Detection Reagent B
Diluent Buffer
Instruction manual
Plate sealer for 96 wells
Pre-coated 96T strip plate
Standard
Stop Solution
TMB Substrate
Wash Buffer (30 x concentrate)

Alternate Names for Human CD133 ELISA Kit (Colorimetric)

AC133 Stargardt disease 4 (autosomal dominant)
AC133
CD133 retinal 2
CD133
PROM1
prominin (mouse)-like 1
Prominin 1
PROML1

Background

Prominin 1 (CD133) is expressed on primitive hematopoietic stem and progenitor cells, retinoblastoma, hemangioblasts, and neural stem cells as well as on developing epithelium. The CD133 positive fraction of human bone marrow, cord blood and peripheral blood have been shown to efficiently engraft in xenotransplantation models, and have been shown to contain the majority of the granulocyte/macrophage precursors, NOD/SCID repopulating cells and CD34 + dendritic cell precursors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Human CD133 ELISA
Kit (Colorimetric)

NBP3-38977

Species: Hu

Applications: ELISA

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for CD133 ELISA Kit (NBP3-38977) (0)

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Additional CD133 Products

Research Areas for CD133 ELISA Kit (NBP3-38977)

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Blogs on CD133.

Check out the latest blog posts on CD133.

Stemness is responsible for onset and metastasis of colorectal cancer
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CD133
Also known as PROM1 and AC133, this gene is located on chromosome 4p15 and encodes CD133, a 120kDa pentaspan transmembrane glycoprotein (5-TM) and presents multiple spliced variants. Prominin-1 (CD133) was the first protein identified as "Prominin"; o... Read full blog post.

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CD31/PECAM-1 was detected in immersion fixed frozen sections of mouse embryo (E13.5) using Goat Anti-Human/Mouse/Rat CD31/PECAM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; NL001) and counterstained with DAPI (blue). Specific staining was localized to developing endothelium. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

CD31/PECAM-1 Antibody [Unconjugated]
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Bioinformatics

Gene Symbol

PROM1

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Antibodies Secondary Antibodies Isotype Controls Proteins and Peptides ELISA Kits Sample Size New Products	About Us Careers Novus Guarantee Contact Us Our Team	Technical Support FAQs Help Finding Antibodies Protocols

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Human CD133 ELISA Kit (Colorimetric)

Species Glossary

Images

Product Details

Order Details

Human CD133 ELISA Kit (Colorimetric) Summary

Applications/Dilutions

Packaging, Storage & Formulations

Kit Components

Alternate Names for Human CD133 ELISA Kit (Colorimetric)

Background

Limitations

Customers Who Viewed This Item Also Viewed...

Publications for CD133 ELISA Kit (NBP3-38977) (0)

Reviews for CD133 ELISA Kit (NBP3-38977) (0)

Product General Protocols

FAQs for CD133 ELISA Kit (NBP3-38977) (0)

Additional CD133 Products

Research Areas for CD133 ELISA Kit (NBP3-38977)

Blogs on CD133.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Review this Product

Bioinformatics