![Western Blot: CCNK Antibody [NBP1-06519] - Whole cell lysate (50 ug) from NIH 3T3, CH27, TCMK-1, BW5147.3, and CT26 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Cyclin Kantibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/CCNK-Antibody-Western-Blot-NBP1-06519-img0016.jpg "Western Blot: CCNK Antibody [NBP1-06519] - Whole cell lysate (50 ug) from NIH 3T3, CH27, TCMK-1, BW5147.3, and CT26 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Cyclin Kantibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1.0 mg/ml |
| Immunogen | The immunogen recognized by this antibody maps to a region between residue 530 and 580 of human cyclin K using the numbering given in entry NP_001092872.1 (GeneID 8812). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | CCNK |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
| Publication using NBP1-06519 | Applications | Species |
|---|---|---|
| Ekumi KM, Paculova H, Lenasi T et al. Ovarian carcinoma CDK12 mutations misregulate expression of DNA repair genes via deficient formation and function of the Cdk12/CycK complex Nucleic Acids Res. 2015-02-20 [PMID: 25712099] (WB, Human) | WB | Human |
Secondary Antibodies |
Isotype Controls |
Research Areas for CCNK Antibody (NBP1-06519)Find related products by research area.
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![Immunohistochemistry-Paraffin: CCNK Antibody [NBP1-06519] - Affinity purified rabbit anti-Cyclin K used at a dilution of 1:5,000 (0.2 ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/CCNK-Antibody-Immunohistochemistry-Paraffin-NBP1-06519-img0017.jpg "Immunohistochemistry-Paraffin: CCNK Antibody [NBP1-06519] - Affinity purified rabbit anti-Cyclin K used at a dilution of 1:5,000 (0.2 ug/ml). Detection: DAB")
![Western Blot: CCNK Antibody [NBP1-06519] - Whole cell lysate (50 ug) from HEK293T, MCF-7, Jurkat, HeLa, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Cyclin K antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/CCNK-Antibody-Western-Blot-NBP1-06519-img0015.jpg "Western Blot: CCNK Antibody [NBP1-06519] - Whole cell lysate (50 ug) from HEK293T, MCF-7, Jurkat, HeLa, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Cyclin K antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.")
![Immunoprecipitation: CCNK Antibody [NBP1-06519] - Detection of human Cyclin K by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Cyclin K antibody NBP1-06519 (lot NBP1-06519-6) used for IP at 6 ug per reaction. Cyclin K was also immunoprecipitated by a previous lot of this antibody (lot NBP1-06519-5) and another rabbit anti-Cyclin K antibody. For blotting immunoprecipitated Cyclin K, NBP1-06519 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.](http://images.novusbio.com/fullsize/CCNK-Antibody-Immunoprecipitation-NBP1-06519-img0014.jpg "Immunoprecipitation: CCNK Antibody [NBP1-06519] - Detection of human Cyclin K by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Cyclin K antibody NBP1-06519 (lot NBP1-06519-6) used for IP at 6 ug per reaction. Cyclin K was also immunoprecipitated by a previous lot of this antibody (lot NBP1-06519-5) and another rabbit anti-Cyclin K antibody. For blotting immunoprecipitated Cyclin K, NBP1-06519 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.")
![Western Blot p27/Kip1 [p Thr157] Antibody](https://images.novusbio.com/images2/p27_AF1555_Western_Blot_5119.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")