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![Western Blot: CCNK Antibody [NBP1-06519] - Whole cell lysate (50 ug) from NIH 3T3, CH27, TCMK-1, BW5147.3, and CT26 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Cyclin Kantibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/CCNK-Antibody-Western-Blot-NBP1-06519-img0016.jpg "Western Blot: CCNK Antibody [NBP1-06519] - Whole cell lysate (50 ug) from NIH 3T3, CH27, TCMK-1, BW5147.3, and CT26 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Cyclin Kantibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Immunohistochemistry-Paraffin: CCNK Antibody [NBP1-06519] - Affinity purified rabbit anti-Cyclin K used at a dilution of 1:5,000 (0.2 ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/CCNK-Antibody-Immunohistochemistry-Paraffin-NBP1-06519-img0017.jpg)
![Western Blot: CCNK Antibody [NBP1-06519] - Whole cell lysate (50 ug) from HEK293T, MCF-7, Jurkat, HeLa, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Cyclin K antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/CCNK-Antibody-Western-Blot-NBP1-06519-img0015.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: CCNK Antibody [NBP1-06519] - Detection of human Cyclin K by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Cyclin K antibody NBP1-06519 (lot NBP1-06519-6) used for IP at 6 ug per reaction. Cyclin K was also immunoprecipitated by a previous lot of this antibody (lot NBP1-06519-5) and another rabbit anti-Cyclin K antibody. For blotting immunoprecipitated Cyclin K, NBP1-06519 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.](http://images.novusbio.com/fullsize/CCNK-Antibody-Immunoprecipitation-NBP1-06519-img0014.jpg)
![Immunohistochemistry: CCNK Antibody [NBP3-35113] - Immunohistochemistry analysis of paraffin-embedded Human breast cancer using CCNK Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35113_rabbit-ccnk-pab-101220241155590.jpg "Immunohistochemistry: CCNK Antibody [NBP3-35113] - Immunohistochemistry analysis of paraffin-embedded Human breast cancer using CCNK Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.")
![Immunohistochemistry: CCNK Antibody [NBP3-35113] - Immunohistochemistry analysis of paraffin-embedded Rat brain using CCNK Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35113_rabbit-ccnk-pab-1012202411552895.jpg)
![Western Blot: CCNK Antibody [NBP3-35113] - Western blot analysis of various lysates using CCNK Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-35113_rabbit-ccnk-pab-1012202411545572.jpg)
![Immunohistochemistry: CCNK Antibody [NBP3-35113] - Immunohistochemistry analysis of paraffin-embedded Mouse spinal cord using CCNK Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35113_rabbit-ccnk-pab-101220241233852.jpg)
![Western Blot: CCNK Antibody (3B7-1B9) [H00008812-M01] - CCNK monoclonal antibody (M01), clone 3B7-1B9. Analysis of CCNK expression in PRC/PRF/5.](http://images.novusbio.com/fullsize/CCNK-Antibody-3B7-1B9-Western-Blot-H00008812-M01-img0007.jpg "Western Blot: CCNK Antibody (3B7-1B9) [H00008812-M01] - CCNK monoclonal antibody (M01), clone 3B7-1B9. Analysis of CCNK expression in PRC/PRF/5.")
![Western Blot: CCNK Antibody (3B7-1B9) [H00008812-M01] - Analysis of CCNK over-expressed 293 cell line, cotransfected with CCNK Validated Chimera RNAi ( Cat # H00008812-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CCNK monoclonal antibody (M01), clone 3B7-1B9 (Cat # H00008812-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/CCNK-Antibody-3B7-1B9-Western-Blot-H00008812-M01-img0005.jpg)
![Western Blot: CCNK Antibody (3B7-1B9) [H00008812-M01] - Analysis of CCNK expression in transfected 293T cell line by CCNK monoclonal antibody (M01), clone 3B7-1B9.Lane 1: CCNK transfected lysate(41 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/CCNK-Antibody-3B7-1B9-Western-Blot-H00008812-M01-img0008.jpg)
![Sandwich ELISA: CCNK Antibody (3B7-1B9) [H00008812-M01] - Detection limit for recombinant GST tagged CCNK is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/CCNK-Antibody-3B7-1B9-Sandwich-ELISA-H00008812-M01-img0006.jpg)
![Western Blot: CCNK Antibody (S1) [H00008812-M03] - Analysis of CCNK expression in transfected 293T cell line by CCNK monoclonal antibody (M03), clone S1. Lane 1: CCNK transfected lysatE (40.8 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/CCNK-Antibody-S1-Western-Blot-H00008812-M03-img0002.jpg "Western Blot: CCNK Antibody (S1) [H00008812-M03] - Analysis of CCNK expression in transfected 293T cell line by CCNK monoclonal antibody (M03), clone S1. Lane 1: CCNK transfected lysatE (40.8 KDa). Lane 2: Non-transfected lysate.")
![ELISA: CCNK Antibody (S1) [H00008812-M03] - Detection limit for recombinant GST tagged CCNK is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/CCNK-Antibody-S1-ELISA-H00008812-M03-img0001.jpg)