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Brg1 Antibody (CL13783)

Images

 
Staining of human liver shows no positivity in hepatocytes as expected.
Orthogonal Strategies: Analysis in human testis and liver tissues using NBP3-24576 antibody. Corresponding SMARCA4 RNA-seq data are presented for the same tissues.
Staining of human endometrial cancer shows strong nuclear positivity in tumor cells.
Staining of human small intestine shows strong nuclear positivity in glandular cells.
Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
Staining of human tonsil shows moderate to strong nuclear positivity in lymphoid cells.
Staining of human ovarian cancer (high grade serous carcinoma) shows strong nuclear positivity in tumor cells.
Staining of human testis cancer (seminoma) shows strong nuclear positivity in tumor cells.
Staining of human uterine cervix shows strong nuclear positivity in squamous epithelial cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications IHC
Clone
CL13783
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

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Brg1 Antibody (CL13783) Summary

Immunogen
This antibody was designed to target the recombinant protein Brg1, with the precise immunogen sequence being withheld for proprietary reasons.
Predicted Species
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SMARCA4
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
Application Notes
For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.2, 40% glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A purified

Alternate Names for Brg1 Antibody (CL13783)

  • ATP-dependent helicase SMARCA4
  • BAF190
  • BAF190A
  • brahma protein-like 1
  • Brg1
  • BRG1-associated factor 190A
  • BRG1SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily A member 4
  • BRM/SWI2-related gene 1
  • EC 3.6.1
  • EC 3.6.4.-
  • FLJ39786
  • global transcription activator homologous sequence
  • homeotic gene regulator
  • hSNF2b
  • Mitotic growth and transcription activator
  • nuclear protein GRB1
  • Protein brahma homolog 1
  • Protein BRG-1
  • RTPS2
  • SMARCA4
  • SNF2
  • SNF2B
  • SNF2-beta
  • SNF2L4
  • SNF2LB
  • SNF2-like 4
  • subfamily a, member 4
  • sucrose nonfermenting-like 4
  • SWI/SNF related, matrix associated, actin dependent regulator of chromatin
  • SWI2
  • transcription activator BRG1

Background

BRG1 is encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Alternatively spliced transcripts have been found for this gene, but their full-length natures have not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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Array NBP3-24576

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Bioinformatics

Gene Symbol SMARCA4