Human beta-NGF ELISA Kit (Chemiluminescence) Summary
Specificity |
This kit recognizes Human NGF in samples. No significant cross-reactivity or interference between Human NGF and analogues was observed. |
Standard Curve Range |
7.81-500 pg/mL |
Sensitivity |
4.69 pg/mL |
Assay Type |
Sandwich-CLIA |
Inter-Assay |
CV% < 8.85% |
Intra-Assay |
CV% < 8.85% |
Spike Recovery |
97-113% |
Sample Volume |
100 uL |
Kit Type |
ELISA Kit (Chemiluminescence) |
Gene |
NGF |
Applications/Dilutions
Packaging, Storage & Formulations
Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
Components
|
- Biotinylated Detection Ab Diluent
- Concentrated Biotinylated Detection Ab (100x)
- Concentrated HRP Conjugate (100x)
- Concentrated Wash Buffer (25x)
- HRP Conjugate Diluent
- Micro CLIA Plate
- Plate Sealer
- Product Manual
- Reference Standard
- Sample Diluent
- Substrate Reagent A
- Substrate Reagent B
|
Alternate Names for Human beta-NGF ELISA Kit (Chemiluminescence)
Background
Nerve growth factor (NGF) is a polypeptide involved in the regulation of growth and differentiation of sympathetic and some sensory neurons, and is composed by 3 types of subunits (alpha, beta and gamma) that specifically interact to form a 7S, 130 kDa molecular weight complex. The beta-NGF subunit is a secreted protein that forms a homodimer before being incorporated into the NGF complex. The human and mouse sequences are very similar and mutations in this gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5). Deregulation of its expression has been associated with allergic rhinitis.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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